Glycocalyx-Directed Enzymatic Hydrogels Unlocks Fibroblast Regeneration to Promote Diabetic Wound Healing biorxiv.org 2026-07-24 Chronic diabetic wounds remain a major clinical challenge because current therapies address infection or supplement growth factors without correcting
Routine FFPE sections support clinically compatible single-nucleus transcriptomics across six human cancer types biorxiv.org 2026-07-24 Tumor cellular composition, including malignant cell states, immune populations, and stromal populations, is increasingly recognized as a determinant
A dual-layer computational framework for prioritising therapeutic candidates targeting extracellular vesicle-mediated immune escape in pancreatic ductal adenocarcinoma biorxiv.org 2026-07-24 Pancreatic ductal adenocarcinoma (PDAC) is an aggressive malignancy characterised by a highly immunosuppressive tumour microenvironment and limited th
Haplotype-based insights into the genetic architecture of net blotch resistance in barley biorxiv.org 2026-07-24 Net blotch, caused by Pyrenophora teres, is a major constraint to barley production worldwide and occurs as two epidemiologically distinct forms: net
Single-kernel near-infrared spectroscopy enables haploid kernel sorting in field and sweet corn using high-oil haploid inducers across diverse donor-inducer combinations biorxiv.org 2026-07-24 Doubled haploid (DH) technology significantly shortens the breeding cycle for developing homozygous inbred lines in maize (Zea mays).
Metabolome genome-wide association study reveals hierarchical and epistatic genetic control of flavonoid metabolism in soybean biorxiv.org 2026-07-24 Metabolic phenotypes are often governed by complex genetic architectures involving both additive and non-additive effects.
Automated design of stiffness-tunable DNA origami hollowframes for self-assembling metamaterials biorxiv.org 2026-07-24 DNA origami offers a route to engineering architected metamaterials with sub-nanometer precision by linking nanoscale building blocks into micron-scal
FMRP prevents Me31B/DDX6-associated repression of long neurodevelopmental mRNAs biorxiv.org 2026-07-24 Long, dosage-sensitive mRNAs encoding neurodevelopmental regulators are selectively dependent on fragile X messenger ribonucleoprotein (FMRP) for effi
Structural basis for emetine inhibition of ribosome translocation in Toxoplasma gondii biorxiv.org 2026-07-24 Apicomplexan parasites, including Toxoplasma gondii and Plasmodium falciparum, are major human pathogens that cause toxoplasmosis and malaria, respect
Identification of the Down syndrome critical region 3 gene as a mammalian cell size regulator biorxiv.org 2026-07-24 Using a genetic screening approach based on an inducible gene-activating system and cell sorting, Down syndrome critical region 3 (DSCR3) was isolated
Spatial control of mitochondrial retrograde signaling by nuclear pore-associated contact sites biorxiv.org 2026-07-24 Mitochondria relay their functional state to the nucleus via retrograde signaling, yet whether the spatial organization of the mitochondrial network p
Impaired synthesis of both telomere strands and adaptive TERT reduction in RTEL1 ATPase-dead cells biorxiv.org 2026-07-24 Regulator of Telomere Elongation Helicase 1 (RTEL1) plays a critical role in telomere replication by disassembling DNA secondary structures such as G-
Self-supervised representations reveal the genetic architecture of human cortical folding biorxiv.org 2026-07-24 Cortical folding emerges during fetal development, is under genetic control, and remains stable throughout life, offering a lasting window into early
An Integrated Multi-omics Single Cell Atlas of the Human RPE and Choroid biorxiv.org 2026-07-24 The retinal pigment epithelium and choroid are critical for supporting the function and maintaining the homeostasis of the outer retina, and their dys
G-quadruplex folding uncouples cis-activation from collateral trans-cleavage by Cas12a biorxiv.org 2026-07-24 The CRISPR/Cas12a system is widely used in nucleic-acid diagnostics because Cas12a couples guide-directed recognition of a cis-target with indiscrimin
Machine Learning-Assisted Evolution of Broadly Functional Enzyme Libraries biorxiv.org 2026-07-24 Biocatalysis offers sustainable solutions to pressing challenges in chemical synthesis by exploiting the remarkable efficiency and selectivity of enzy
Intratumoral dose heterogeneity promotes adaptive anti-tumor immunity and predicts clinical response to radiopharmaceutical therapy biorxiv.org 2026-07-24 Radiopharmaceutical therapies (RPT) deliver non-uniform radiation dose in tumors and the impact of this on response is poorly understood.
Metabolic engineering of Escherichia coli to modulate hydrogen sulfide levels in the mammalian gut biorxiv.org 2026-07-24 Hydrogen sulfide (H2S) is a microbiota-derived metabolite in the gastrointestinal tract implicated in a number of diseases.
Network-scale disruption of RNA-protein interactions by a bioaccumulating small-molecule drug biorxiv.org 2026-07-24 Many small-molecule drugs accumulate in both host and gut microbial cells.
Network topology determines neuronal phase-of-firing codes biorxiv.org 2026-07-24 Brain rhythms organize neural activity in time.
Microbial Immunomodulatory Function is Altered in Inflammatory Bowel Disease Independent of Microbial Composition biorxiv.org 2026-07-24 Inflammatory bowel diseases (IBD) are chronic and relapsing immune-mediated conditions in which the NF-{kappa}B and STAT3 signalling pathways play cen
Chromosome-scale CRISPR screening reveals secretory pathway genes as drivers of aneuploidy-mediated antifungal tolerance biorxiv.org 2026-07-24 The gain or loss of chromosomes in eukaryotes often drives aberrant phenotypes by altering the expression levels of hundreds or thousands of genes.
Intersection of genotype and environment on the dynamics of the virome and microbiome of an estuarine cnidarian biorxiv.org 2026-07-24 Estuaries host a rich diversity of microorganisms, creating opportunities for animal-microbe interactions that shape host physiology.
How are evolutionarily young and old proteins distributed in sequence space? biorxiv.org 2026-07-24 Protein sequence space is vast due to the combinatorial diversity of 20 amino acids.
Mitochondrial introgression in North American red-backed voles is facilitated by co-introgression at nuclear-encoded mitochondrial genes biorxiv.org 2026-07-24 Mitochondrial genomes encode for the proteins and RNAs that serve as the basis for aerobic respiration and energy production.
Recombination on 'non-recombining' sex chromosomes: empirical insights from the blackspotted stickleback biorxiv.org 2026-07-24 One hallmark of sex chromosome evolution is recombination suppression driven by inversions.
Proteogenomic analysis of the differential stability of cardiac protein isoforms biorxiv.org 2026-07-24 Alternative splicing is an important regulatory layer in gene expression, but knowledge on the isoform protein molecules continue to lag their canonic
A Microgel-Based Platform for Tunable Expansion and Function of γδ T-cells biorxiv.org 2026-07-24 Current {gamma}{delta} T-cell expansion protocols often sacrifice functionality for yield and largely ignore the context of activation.
Epigenetic Regulation of Stable SARS-CoV-2 RBD-sfGFP Expression in Primary Human Splenic Fibroblasts biorxiv.org 2026-07-23 Background: Recombinant expression of the SARS-CoV-2 receptor-binding domain (RBD) is essential for vaccine development, serological diagnostics, and
The Metabarcoding Analysis Pipeline (MAP): Simple, accurate, and flexible metabarcoding biorxiv.org 2026-07-23 Current metabarcoding pipelines are inflexible with respect to study design and are poorly suited to long-read sequence data.