Genetic background diversifies mutational paths but converges on membrane depolarization for bleomycin resistance in yeast biorxiv.org 2026-08-01 Drug resistance evolution is shaped by both selective agent and genetic background in which resistant mutations arise, but most experimental evolution
Evolution of a large and diverse phospholipase gene cluster that defines the plant pathogenic genus Ceratocystis biorxiv.org 2026-08-01 Many Ceratocystis species cause cankers and unique vascular wilt diseases, often on a broad and unpredictable range of plant hosts.
Enhancing Recombinant Vector Assembly Efficiency: A Novel Methodological Approach biorxiv.org 2026-08-01 With the rapid development of modern biotechnology, DNA vectors have become fundamental tools for inserting, transferring, and expressing specific gen
Combinatorial sequence elements fine-tune mitochondrial protein import to facilitate dual localization biorxiv.org 2026-08-01 The dual targeting of mitochondrial proteins regulates a host of cellular processes, including metabolism, cofactor biosynthesis, mitophagy, and stres
An alphabet of allostery: a transferable transfer entropy contact patterns predicts allosteric-site character and conformational rewiring biorxiv.org 2026-08-01 Allostery is increasingly understood as the propagation of dynamic information through a protein, yet the computational descriptors of that flow are c
Reconstructing lineage-constrained gene-program dynamics with PhyloFM biorxiv.org 2026-08-01 Cell-fate dynamics are defined not only by where cells end, but by when fate programs emerge, diverge, and reshape population structure.
Equi-depth pooling for standardized and cost-effective Adaptive Immune Receptor Repertoire sequencing (AIRRseq) biorxiv.org 2026-08-01 Bulk B- and T-cell adaptive immune receptor (BCR/TCR) repertoire sequencing (AIRRseq) enables comprehensive analysis of adaptive immune diversity, but
DEAR-OWL: a fully browser-based hybrid resource for instant or precise differential gene expression analysis biorxiv.org 2026-08-01 Motivation: Differential gene expression analysis (DEA) via RNA sequencing (RNA-seq) is essential but remains challenging for wet-lab biologists due t
A hyperspherical deep Bayesian model for interpretable clustering and relationship prediction in microbiome multi-omics integration biorxiv.org 2026-08-01 The microbiome plays a significant role in the development and progression of many diseases, yet extracting interpretable insights from multi-omics da
Optimized Multiple Circular Sequence Alignment for Cyclic Peptide Motif Discovery biorxiv.org 2026-08-01 Head-to-tail (H2T) cyclized peptides are an increasingly important modality in drug discovery, combining high target affinity and selectivity with met
Joint modeling of multi-timepoint spatial observations for time-resolved spatial-unit-specific gene regulatory network inference biorxiv.org 2026-08-01 Background: How gene regulatory programs reorganize across space and time is central to development and disease, but current methods infer regulatory
Systematic assessment of sequencing depth requirements for Hi-C-derived metrics biorxiv.org 2026-08-01 Hi-C experiments produce genome-wide chromatin contact maps from which structural features can be quantified across multiple genomic scales, ranging f
MetaAMI: A Novel Meta-Learning Approach for Predicting In-Hospital Mortality in Acute Myocardial Infarction biorxiv.org 2026-08-01 Acute myocardial infarction (AMI) is one of the leading cardiovascular diseases worldwide and remains a major cause of mortality.
CPPLocPred: Subcellular Localization of Cell-Penetrating Peptides biorxiv.org 2026-08-01 Cell-penetrating peptides (CPPs) are widely used to deliver therapeutic cargoes into cells.
Automating 3DED data processing at eBIC biorxiv.org 2026-08-01 Three-dimensional electron diffraction (3DED) is an emerging and useful technique for solving molecular structures of small and biological macromolecu
SPINDLE: Unlocking protein dynamics from single-field NMR relaxation data using a deep learning ensemble. biorxiv.org 2026-08-01 A protein's function is derived from its three-dimensional structure and the motions of the atoms about that structure.
Toward scalable ambulatory light dosimetry: sensor-placement bias under naturalistic conditions biorxiv.org 2026-08-01 Background: Personal light dosimeters enable exposure assessment under free-living conditions, but sensors are rarely positioned near the eyes-the rel
Xanthophyll acyl esters in pepper (Capsicum annuum): Delineating the role of two xanthophyll acyltransferases CaPYP1 and CaPYP1-like via gene silencing and genetic complementation. biorxiv.org 2026-08-01 Pepper fruit accumulated xanthophyll acyl esters during ripening.
Delineating the posterior bank of the feline auditory cortex based on neurofilament proteins expressing SMI-32 biorxiv.org 2026-08-01 The feline auditory cortex is understood to consist of 13 distinct subregions with unique anatomical and functional properties.
Macroscale dynamics of EEG microstates determine the periodic and aperiodic features of the neural power spectrum biorxiv.org 2026-08-01 The global signal characteristics of scalp-recorded electroencephalography (EEG) are composed of periodic oscillatory rhythms and aperiodic broadband
An end-to-end framework for single-cell-resolution, whole-transcriptomic spatial profiling in post-mortem human brain biorxiv.org 2026-08-01 Single-cell resolution spatial transcriptomics enables transcriptome-wide molecular profiling within intact tissue architecture, providing unprecedent
cGMP signaling regulates context-dependent sensory valence in C. elegans biorxiv.org 2026-08-01 An animal's response to chemosensory cues depends on the animal's prior experience, internal state, or life stage.
Tumor-specific CD4 T cells cooperate with myeloid cells to remodel the pancreatic tumor microenvironment and enable effective immunotherapy biorxiv.org 2026-08-01 We interrogate antigen-specific CD4 T cells during immunotherapy in pancreatic ductal adenocarcinoma.
Destabilization of intratumor Tregs by CTLA-4 engagement confers anti-CTLA-4-driven immunotherapy biorxiv.org 2026-08-01 Immune checkpoint inhibitors (ICI) that are antibodies against CTLA-4 have achieved therapeutic effects on multiple types of cancers, but the mechanis
Fermentation-Derived Metabolites Shape Host Biology to Attenuate Severity of Inflammatory and Metabolic Disease biorxiv.org 2026-08-01 Fermented foods are among the few dietary interventions shown to increase gut microbiome diversity and reduce systemic inflammation in healthy adults,
Convergent genomic variation and wild-type composite RNA-seq profiling identify candidate modules associated with 3-nitropropionic acid utilization in Leclercia barmai biorxiv.org 2026-08-01 3-Nitropropionic acid (3-NPA) is a toxic nitroaliphatic compound, but the genomic basis of bacterial adaptation to 3-NPA remains incompletely resolved
A zebrafish leprosy model identifies interferon and plasminogen signaling as survival determinants in mycobacterial infection biorxiv.org 2026-08-01 Although many genes are associated with leprosy, a skin and nerve infection by Mycobacterium leprae, the function of most of these genes in infection
Multicellular Programs Associated with Right Ventricular Adaptation in Pulmonary Arterial Hypertension biorxiv.org 2026-08-01 Background: Right ventricular (RV) adaptation determines outcomes in pulmonary arterial hypertension (PAH), yet multicellular molecular programs assoc
Nitric oxide inhibits platelet adhesion to platelet-microparticles through reducing integrin αIIbβ3 activation biorxiv.org 2026-08-01 Increased platelet microparticle (PMP) levels in individuals with risk factors for cardiovascular disease correlate with clinical outcomes in these pa
An inversion-rich genome shapes admixture outcomes, generating a mosaic of lineage-specific ancestries in the European sardine biorxiv.org 2026-08-01 The role of chromosomal inversions in modulating the outcomes of admixture between divergent evolutionary lineages remains poorly understood.
Asynchronous structural rearrangements govern constriction of the human nuclear pore complex biorxiv.org 2026-08-01 The nuclear pore complex (NPC) is responsible for transport between the cytoplasm and nucleus.
How a single sperm protein recognizes highly divergent egg coat components biorxiv.org 2026-08-01 For successful fertilization, sperm must recognize and penetrate the egg coat, called vitelline envelope (VE) or, in mammals, zona pellucida (ZP).
Maintenance of phenotypic divergence in two sympatric monkeyflowers with weak reproductive isolation biorxiv.org 2026-08-01 Sympatric species often exhibit permeable reproductive barriers, yet many of them coexist as phenotypically distinct entities.
Integrative genomics of the siphonophore Physalia utriculus reveals the regulatory logic of colonial division of labour and the molecular basis of venom activity biorxiv.org 2026-08-01 How a single genome gives rise to specialised multicellular individuals that function as an integrated organism remains a fundamental question in the
Decorin-mediated Extracellular Matrix Stabilization Maintains Cartilage Integrity During Aging biorxiv.org 2026-08-01 Aging is a primary risk factor for osteoarthritis (OA), yet the mechanisms that preserve extracellular matrix integrity in long-lived connective tissu
An Open-Source, Excitation-Resolved UV-NIR Imaging Platform for Bioluminescence, Luminescence-Decay, and Chemical Discrimination Measurements biorxiv.org 2026-08-01 Multispectral imaging is a cornerstone of chemical imaging, with bioluminescence, fluorescence, and phosphorescence imaging underpin much of preclinic
Stable and robust differentiation programs are predominantly irreversible biorxiv.org 2026-08-01 Multicellular differentiation programs are almost always irreversible, with cells rarely revisiting states observed during earlier developmental stage
FITdb, an Integrated Functional Immunogenomics and Transcriptomics Database biorxiv.org 2026-08-01 Genetic screens in immune cells enable the systematic interrogation of gene function at scale, uncovering key regulators of cell functions such as tum
Carbohydrate degradation machineries in lichen fungal symbionts reveal distinct symbiotic footprints across Ascomycota biorxiv.org 2026-08-01 Lichens--the archetypal symbiosis--have long been known for their nutritional relationship, in which the photoautotrophic partners subsidize the carbo
Sound-shape correspondences in macaques reveal evolutionary roots of sound symbolism biorxiv.org 2026-08-01 Humans reliably associate certain speech-like sounds with visual shapes, most notably in the Bouba-Kiki Effect, where rounded and spiky shapes are mat
Pangenome Graph Node-Phenotype Association shows GWAS-like quality results with only few individuals biorxiv.org 2026-08-01 Purpose: We introduce GraNPA, standing for Graph Node-Phenotype Associa- tion, a method performing a GWAS-like analysis on a pangenome variation graph
Millisecond-scale, single-neuron credit assignment in a songbird biorxiv.org 2026-08-01 Learning an adaptive behavior requires identifying which actions, in which contexts, lead to particular outcomes.
Disrupted Developmental Trajectory of Ultrasonic Vocalizations in a Rat Model of Fragile X Syndrome biorxiv.org 2026-08-01 Communication deficits are a defining feature of autism spectrum disorder (ASD) and among the earliest detectable markers of atypical neurodevelopment
Distinct transcriptomic and hierarchical organization associated with brain hyperconnectivity and hypoconnectivity in autism spectrum disorder biorxiv.org 2026-08-01 Functional hyperconnectivity and hypoconnectivity in autism spectrum disorder (ASD) are typically treated as opposing expressions of a single circuit-
Cross-species neural co-culture uncovers metabolic signatures of cellular crosstalk biorxiv.org 2026-08-01 Primates are distinguished by large brains relative to body size, with humans showing the greatest expansion.
Common schizophrenia heritability concentrates in an evolutionarily young, brain-regulatory subset of fine-mapped credible-set variants biorxiv.org 2026-08-01 Common variation explains a substantial fraction of schizophrenia heritability, yet why these risk alleles persist remains unresolved.
A TmaT-AftD interaction is required for the CmpL4 mycomembrane biogenesis pathway in Corynebacterium glutamicum. biorxiv.org 2026-07-31 Bacteria in the Mycobacteriales order like mycobacteria and corynebacteria surround themselves with a multilayered cell envelope.
Cpf1(Cas12a)-based genome editing in the filamentous cyanobacterium Nostoc punctiforme biorxiv.org 2026-07-31 The filamentous cyanobacterium Nostoc punctiforme is a key model organism used to study several aspects of cyanobacterial biology, including developme
Colistin resistance-mediated lipopolysaccharide modification in Klebsiella pneumoniae modulates host inflammatory response biorxiv.org 2026-07-31 Resistance to the polymyxin antimicrobial colistin in Gram-negative bacteria is associated with a modification of the immunogenic lipid A moiety of th
Direct targeting of the WAVE-regulatory complex and the actin cytoskeleton by the Anaplasma phagocytophilum effector, AnkA biorxiv.org 2026-07-31 The obligate intracellular tick-borne pathogen Anaplasma phagocytophilum is unusual in its tropism for neutrophils, within which it survives and repli