Rich structure alphabets enable highest accuracy protein search biorxiv.org 2026-07-30 Protein structure databases have grown from thousands of experimentally determined structures to hundreds of millions of AI-predicted models, creating
Deciphering the Effect of Melittin on Murine Cervical Cancer Cells Based on Transcriptomic Investigation biorxiv.org 2026-07-30 Abstract: Melittin, a highly active natural antimicrobial peptide derived from honeybee venom, holds immense pharmacological potential against solid t
Minimally invasive monitoring of clonal evolution through integrated single cell and ctDNA analysis biorxiv.org 2026-07-30 Abstract Circulating cell-free DNA (cfDNA) offers a minimally invasive lens into temporal tumor evolution.
RNA-directed DNA methylation controls seed development and heat stress memory in barley biorxiv.org 2026-07-30 Plant specific RNA-directed DNA methylation (RdDM) mediates the DNA methylation of specific DNA sequences directed by 24-nucleotide long (nt) small in
Photosynthetic assimilate determines branch size and biomass more than branch number in Arabidopsis biorxiv.org 2026-07-30 Shoot branching is a primary determinant of plant form and crop yield, yet whether auxin or carbon supply is the proximal regulator of branching remai
Plant regulator of flower bud differentiation in in vitro plants of Cymbidium tortisepalum var. longibracteatum with TDZ as the key initiator biorxiv.org 2026-07-30 [Background] Cymbidium tortisepalum var.
Teaching adipose tissue as an organ system: addressing weight bias and enhancing anatomical understanding through AdipoAtlas biorxiv.org 2026-07-30 Adipose tissue (body fat) is increasingly recognized as a dynamic organ system essential to human health, with distinct depots that serve specialized
Exosome-Derived Proteomic Signatures Highlight Pathogenic Mechanisms in Moyamoya Disease biorxiv.org 2026-07-30 Background: The etiology and molecular mechanisms of Moyamoya disease (MMD) remain unclear.
Low effective dimensionality of the human functional connectome does not invalidate lesion network mapping biorxiv.org 2026-07-30 Van den Heuvel et al.
Circadian clock regulates intestinal epithelial cell differentiation via NOTCH/Hes1 oscillations biorxiv.org 2026-07-30 The circadian clock regulates diverse cellular processes, including intestinal epithelial cell (IEC) proliferation.
Nicotinamide N-methyltransferase couples inflammation and epigenetic remodelling to hepatic fibrosis biorxiv.org 2026-07-30 Chronic inflammation is a key driver of progression from benign steatosis to metabolic dysfunction-associated steatohepatitis (MASH) and liver fibrosi
NFATc2 potentiates DNA double-strand breaks repair by interaction with Ku80 in radiotherapy patients biorxiv.org 2026-07-30 A fraction of patients treated with radiotherapy are known to be more sensitive to ionizing radiations.
Chaperone condensates buffer the heat shock response against pleiotropic inputs biorxiv.org 2026-07-30 Stress response pathways can be specific, dedicated to one stimulus, or pleiotropic, funneling distinct perturbations into a shared program.
In vivo reconstruction of the cell lineage history of a developing mouse with DNA Typewriter, from zygote to late organogenesis biorxiv.org 2026-07-30 The complete cell lineage of C. elegans, mapped over four decades ago, was tractable because the animal is small, transparent, and lineage invariant.
The effect of DNA methylation on DNA structure and DNA-small molecule interactions biorxiv.org 2026-07-30 The methylation of cytosines at the 5th position (d5mC) is one of the most common epigenetic modifications, and alterations in methylation profile of
Resolving Immune Lineage and Cell-State Heterogeneity in Human PBMCs via Mass Spectrometry-Based Single-Cell Proteomics biorxiv.org 2026-07-30 Single-cell proteomics (SCP) currently lacks validated benchmarking standards, and cell annotation often relies on transcriptomic proxies.
Exploring the Impact of S. aureus Lipase Activity on Intra- and Extracellular Lipids biorxiv.org 2026-07-30 Previous research has shown that mammalian fatty acids (FAs) can influence antibiotic tolerance of Staphylococcus aureus, yet many of these studies ov
A Tenebrio molitor model for Talaromyces marneffei infection and the importance of host cues for dimorphic switching biorxiv.org 2026-07-30 There is a growing body of research demonstrating the utility of invertebrate models for studies of fungal pathogenesis.
Branched C7-Substituted 7-Deaza-SAH Analogues Occupy the Entire SAM-Binding Pocket of Mpox Virus VP39 and Dengue Virus NS5 Methyltransferases biorxiv.org 2026-07-30 Viral RNA-cap MTases are attractive targets for antiviral drug development.
Quantitative Ultrasound Analysis of Gas Embolic Disease in Bycaught Sea Turtles biorxiv.org 2026-07-30 Objectives Bycatch-related decompression after forced submersion can result in severe gas embolic disease in sea turtles.
Decoupling axonal regrowth and branching through Imp-dependent RNA regulation during neuronal remodeling biorxiv.org 2026-07-30 Structural remodeling of neuronal projections in response to developmental cues, injury, or disease is essential for adaptive circuit rewiring.
Time Course and Microcircuit Mechanisms of Primary Motor Cortex Dysfunction in Progressive Parkinsonism biorxiv.org 2026-07-30 Dysfunction of the primary motor cortex (M1) has long been implicated in the pathophysiology of Parkinsons disease (PD), mostly as a link by which abn
CORSICA: Reproducible Suppression of Cochlear Implant Artifacts in EEG evoked by Continuous Speech biorxiv.org 2026-07-30 Objective. Electroencephalography (EEG) is a key tool for studying auditory processing in cochlear implant (CI) users.
Perfusion quality does not necessarily predict ultrastructural preservation after hyperosmotic brain perfusion biorxiv.org 2026-07-30 Perfusion fixation is widely used in neuroscience to prepare mammalian brain tissue for histological and ultrastructural analysis.
Boldine inhibits SARM1 NADase Activity and Preserves Axonal Integrity After Nerve Injury biorxiv.org 2026-07-30 Wallerian degeneration of anucleated axonal segments is driven by SARM1, which depletes axonal NAD+, disrupting energy metabolism and triggering self-
Implementation of ddaE neuron growth mechanism in graph grammar replicates biological features biorxiv.org 2026-07-30 Dendrites develop branching patterns that are critical for their function, yet the mechanisms guiding arbor morphology remain incompletely understood,
Adult Clock Neuron Somatic Neuropeptide Release and Cytonemes Regulate Sleep biorxiv.org 2026-07-30 Drosophila s-LNv clock neurons promote nighttime sleep by releasing the neuropeptide sNPF to activate sNPF receptors (sNPF-Rs) on l-LNv clock neurons.
High precision animal stereotaxis with 3D polychromic photogrammetry biorxiv.org 2026-07-30 Stereotaxic brain surgery is a foundational neurosurgical technique used to deliver chemical, pharmacological, or genetic material to specific brain r
2,6-diaminopurine enhances Aqp4 stop codon readthrough and AQP4 perivascular localization in the brain biorxiv.org 2026-07-30 In the brain, the water channel Aquaporin 4 (AQP4) is largely restricted to astrocytes, enriched at perivascular astrocytic processes, and involved in
Age-dependent peripheral nerve and Schwann cell abnormalities in a mouse model of late-onset spinal muscular atrophy biorxiv.org 2026-07-30 Spinal muscular atrophy (SMA) is increasingly recognized as a multisystem disorder involving non-neuronal cells, yet the role of Schwann cells (SCs) i
Microbial evolution, biogeochemical functions, and environmental adaptations in a desert saline lake on the Qinghai-Tibet Plateau biorxiv.org 2026-07-30 The Eboliang Hu saline lakes in the hyper-arid Qaidam Basin is a high-altitude, weakly acidic hypersaline system with strong environmental gradients a
A recent domestication event in Penicillium biforme, independent of the emblematic cheese mold P. camemberti biorxiv.org 2026-07-30 Domestication of molds for cheese production has repeatedly shaped Penicillium fungi, most notably giving rise to the emblematic P.
The modular origin and evolutionary expansion of the enigmatic DGF-1 protein family in trypanosomatids biorxiv.org 2026-07-30 Large multigene families are a hallmark of trypanosomatid genomes and play major roles in genome plasticity and parasite adaptation.
LLM-powered Functional Gene Set Summarization with genesetGPT biorxiv.org 2026-07-30 Transcriptomics datasets generated using next-generation sequencing techniques such as single cell RNA-sequencing (scRNA-seq) and spatially-resolved t
Bordetella pertussis BctCBA Mediates Citrate-Dependent Zn2+ and Ni2+ binding biorxiv.org 2026-07-30 Bordetella pertussis, the causative agent of whooping cough, is a reemerging public health threat.
Information-theoretic Limits on Programmatic Specification of Biological Systems biorxiv.org 2026-07-30 The central problem of biology is the origin of biological organization.
Analytical methodology for unveiling the physical effect of detergents on the function of full-length membrane proteins using a single-molecule tracking system biorxiv.org 2026-07-30 G protein-coupled receptors (GPCRs) are important targets for drug discovery because they are the largest and most diverse family of membrane proteins
Kinase inhibitors can change protonation or tautomeric state upon binding biorxiv.org 2026-07-30 The binding affinity of a ligand to a protein is influenced by the protonation and tautomeric states of both partners.
Predicting tuberculosis relapse based on 28-day CFU, RS ratio, and/or drug contribution for novel regimens in the relapsing mouse model biorxiv.org 2026-07-30 Treatment shortening in tuberculosis therapy is needed, but testing all novel antibiotic combinations is unfeasible.
Genomic Profiling of Estrogen-Related Receptor Identifies Adult Adipocyte-Specific Targets in Drosophila biorxiv.org 2026-07-30 Adipose tissue is a key metabolic organ for carbohydrate and lipid metabolism.
Unraveling Wheat Aphid Diversity in Bangladesh Through Integrative Genetic and Morphological Approaches biorxiv.org 2026-07-30 Accurate species identification is fundamental for understanding insect pest diversity and developing effective management strategies.
Direction-Specific Effects of Biphasic Transcranial Magnetic Stimulation on Cortical and Cortico-spinal Excitability biorxiv.org 2026-07-30 Aims & Methods: Transcranial magnetic stimulation (TMS) is a well-established tool for inducing cortical excitation.
Reproducible transversal mouse brain sections using low-cost 3D printable resin matrix biorxiv.org 2026-07-30 Rodent brain matrices that produce coronal or sagittal brain sections for histology confer reproducibility and enable high throughput processing of ti
A horizontally acquired and recurrently expanded glycoside hydrolase subfamily across leafhoppers biorxiv.org 2026-07-30 Horizontal gene transfer from bacteria is a known source of metabolic novelty in insects, yet how these acquisitions diversify and persist over evolut
Deep Brain Stimulation Microelectrodes as a Source of Human Subcortical RNA: Validation of a Low-Input Transcriptomic Protocol biorxiv.org 2026-07-30 Background: Understanding the molecular basis of Parkinson ' s disease (PD) phenotypic heterogeneity maybe improved by in vivo access to deep brain ti
Within-host succession from an OXA-48-like to an NDM-type carbapenemase in clonal ST361 Escherichia coli recovered from sequential urinary and bloodstream infection biorxiv.org 2026-07-30 Carbapenem-resistant Escherichia coli (CREc) recovered sequentially from one patient typically retain the same carbapenemase, with escalating resistan
Expanded unbiased population-genomic summary statistics in pixy biorxiv.org 2026-07-30 Estimates of population-genetic summary statistics are often computed from variant-only VCFs, which commonly omit invariant sites (i.e.
Interpretable spatial ecological dynamics reveal ecological retention escape and amplification in pediatric leukemia biorxiv.org 2026-07-30 Spatial transcriptomics enables tumor ecosystems to be examined within their native tissue architecture, yet many computational approaches remain prim
Modular counterplays shape condensation and signaling of chimeric antigen receptor biorxiv.org 2026-07-30 Chimeric antigen receptor-engineered T (CAR T) cell therapy has achieved remarkable clinical efficacy against hematological malignancies but exhibits
Untargeted metabolic analysis reveals intraspecific and organ-specificchemodiversity in Solanum dulcamara biorxiv.org 2026-07-30 Background and Aims. The genus Solanum including the wild species S.