Prevention of mRNA vaccine-induced anaphylaxis by peripheral cyclooxygenase inhibitors in an anti-PEG hyperimmune pig model: clinical relevance for nanomedicine-induced infusion reactions biorxiv.org 2026-05-29 Anti-polyethylene glycol (PEG) hyperimmune pigs, immunized against PEG, provide a sensitive experimental model for the rare anaphylactic reactions ind
Southeastern Australian Montane Fens Harbour Distinct Microbial Communities Rich in Novel Diversity biorxiv.org 2026-05-29 Montane fens are rare and microbiologically poorly characterised wetland ecosystems in south-eastern Australia, and their microbial communities remain
Fermented Botanical Product Modulates Soil Bacterial Communities and Enhances Plant-Growth-Promoting Activity for Sustainable Agriculture biorxiv.org 2026-05-29 Although biostimulants have attracted attention for sustainable agricultural systems, their efficacy remains poorly understood.
Tripartite synergy - Metabolic crosstalk between two bacterial mutualists and a marine microalga promotes algal fitness biorxiv.org 2026-05-29 Marine microalgae form major parts of phytoplankton and are highly relevant for global CO2 fixation.
Delivery of defective interfering RNA antivirals to the lungs using hyperbranched poly(beta-amino ester) nanoparticles biorxiv.org 2026-05-29 Hyperbranched poly(beta-amino ester) (hPBAE) nanoparticles represent a promising platform for nucleic acid delivery, particularly to the lungs.
Structural basis for recognition of a gonococcal lipooligosaccharide epitope by monoclonal antibody 2C7 informs vaccine and immunotherapeutic design biorxiv.org 2026-05-29 Neisseria gonorrhoeae causes gonorrhea and poses a growing global health threat driven by antimicrobial resistance and the lack of an effective vaccin
Feeding-mode-defined microbial communities modulate prebiotic responses and alters colonic motility in early life biorxiv.org 2026-05-29 Prebiotic supplementation a well-established impact on early-life gut microbiota, yet how substrate type and baseline community structure interact to
Genome-resolved metagenomics reveals the potential for selection for antibiotic resistance due to metal pollution in soil microbial communities near a copper-nickel mine site in Botswana biorxiv.org 2026-05-29 Mismanagement and metal pollution are high in countries in the global south and often create challenging, extreme conditions for soil microbial commun
TDP1 drives MRE11-dependent and independent end resection during replication stress by TOP2 poisons biorxiv.org 2026-05-29 DNA-Topoisomerase crosslinks are lesions formed by proteins covalently bound to DNA, and these complexes directly impede transcription and replication
Quantifying circulation and antibody cross-reactivity for co-circulating flaviviruses: the case of Usutu and West Nile virus in blackbirds biorxiv.org 2026-05-29 Serological testing is important for assessing past exposure and immunity, but interpretation can be complicated by antibody cross-reactivity between
Development of a CRISPR/Cas9-mediated transformation procedure for the wheat pathogen Zymoseptoria tritici biorxiv.org 2026-05-29 Zymoseptoria tritici is the causal agent of Septoria tritici blotch (STB), one of the most destructive diseases of wheat worldwide. Although the Z.
Mutational and functional heterogeneity of homology-directed repair deficiency and clinical implications biorxiv.org 2026-05-29 Homology-directed repair deficiency (HRd) encompasses mutations in multiple genes yet is treated clinically as a single entity.
Graph-based pangenome provides insights into the adaptive evolution of Cucurbita pepo biorxiv.org 2026-05-29 Understanding how crops respond to environmental variation is crucial for biodiversity conservation and food security.
An SE(3)-equivariant Crystal Structure Prediction Framework for Prospective Identification and Development of Bioactive Nucleoside Self-Assembling Materials biorxiv.org 2026-05-29 Flexible organic small molecules can assemble into functional materials whose properties are intrinsically governed by their crystal structures, yet e
NEO-AAV: an engineered extracellular vesicle-enveloped AAV platform for activation-coupled T cell transduction and CAR-T cell generation biorxiv.org 2026-05-29 Efficient and targeted delivery of genetic cargo remains a major barrier for gene and cell therapies, particularly in primary human T cells that are i
Computational Structure Modeling, Functional Characterization, and Identification of Potential Inhibitors for the cyclic-di-GMP Mediated Biofilm Forming Membrane Protein in Vibrio cholerae biorxiv.org 2026-05-29 Cholera, caused by Vibrio cholerae, continues to pose a serious global public health challenge, with its impact worsened by rising antibiotic resistan
Solvent ordering in a fully packaged RNA bacteriophage and the mechanics of genome delivery biorxiv.org 2026-05-29 Self-assembly of an RNA virus requires a thermodynamic state stable enough to confine the highly charged genome yet primed for genome delivery.
DeepDiffusion: a Physics-Informed Neural Network for Heterogeneous Facilitated 1D Diffusion biorxiv.org 2026-05-29 Single-molecule fluorescence microscopy combined with optical tweezers has enabled the direct observation of diffusing proteins on tethered DNA.
Bright Probes, Blurred Metabolism: Navigating Fluorescent Protein Cross-Excitation in NADH FLIM biorxiv.org 2026-05-29 Fluorescence lifetime imaging microscopy (FLIM) of endogenous NAD(P)H enables the label-free assessment of cellular metabolic state.
Inhibition of hexokinase 2 undermines cartilage health and accelerates osteoarthritis biorxiv.org 2026-05-29 Objective: Enhanced glycolysis is a metabolic hallmark of chondrocytes in osteoarthritis (OA); however, the roles of the glycolytic rate-limiting enzy
Synthetic cell-cell adhesion provides benefits of proximity without diffusion-related costs biorxiv.org 2026-05-29 Multicellularity provides many advantages over unicellularity, but accessing those benefits with synthetic biology approaches is challenging due to po
The mechanosensitive channel MscS links lipoteichoic acid synthesis alterations to beta-lactam sensitivity in Staphylococcus aureus biorxiv.org 2026-05-29 Lipoteichoic acid is an essential cell envelope polymer in Staphylococcus aureus and contributes to beta-lactam resistance in methicillin-resistant S.
A 667-nucleotide sequence in the SARS-CoV-2 nsp15 coding region promotes genome encapsidation biorxiv.org 2026-05-29 Coronavirus genome encapsidation depends on cis-acting RNA elements that interact with viral structural proteins.
Phenological Plasticity without Directional Change: Tropical Hornbills Track Temperature but Maintain Reproductive Stability biorxiv.org 2026-05-29 Climate change is altering breeding phenology in birds worldwide, yet tropical systems remain understudied despite harbouring the greatest avian diver
Multispecies Mixtures: An Individual-Centered Quantitative Genetic Framework for Complex Plant Neighborhoods biorxiv.org 2026-05-29 Modern agriculture faces major sustainability challenges, including stagnating yields, dependence on fossil resources, and severe environmental impact
Decoding universal principles of codon-mediated regulation of gene expression biorxiv.org 2026-05-29 Codon usage determines gene expression levels, yet its universal principles remain elusive.
High-Dimensional Sensitivity Analysis for Genomic Studies: An Adversarial Framework for Learning Worst-Case Latent Confounders biorxiv.org 2026-05-29 High-dimensional genomics studies are frequently confounded by unmeasured biological processes that obscure disease-specific signals.
Identification of genes important for response of Pseudomonas aeruginosa biofilms to ciprofloxacin exposure biorxiv.org 2026-05-29 Pseudomonas aeruginosa is an opportunistic pathogen that can cause severe infections in immunocompromised individuals, such as patients with cystic fi
Life-history traits may buffer genetic erosion under isolation in mountain sky-island systems biorxiv.org 2026-05-29 Sky-island systems provide natural case studies for understanding how geography and Quaternary history shape genomes, phenotypes and life-history trai
Programmable DNA integration with New-to-Nature tools using Computational Protein Design biorxiv.org 2026-05-29 Programmable integration of large DNA cargo ([≥] 2 kb), without inducing double-strand breaks, remains challenging for genome editing technologies.
Bridging the gap in embryogenesis research: North Bornean orangutan-derived blastoids as models for human development doi.org
Bioethical Assessment of Research with Humanoid or Humanized Biological Entities with Uncertain Moral Status doi.org
The establishment of human embryonic stem cell lines demonstrated that chimeric embryos can self-correct doi.org
Single-cell analysis of human embryos reveals diverse patterns of aneuploidy and mosaicism biorxiv.org
A multi-omics genome-and-transcriptome single-cell atlas of human preimplantation embryogenesis reveals the cellular and molecular impact of chromosome instability biorxiv.org
Expression bias in retinoic acid responsive genes defines variations in neural differentiation of human pluripotent stem cells biorxiv.org
Organoid-based single-cell spatiotemporal gene expression landscape of human embryonic development and hematopoiesis biorxiv.org
Stem cell-derived mouse embryos develop within an extra-embryonic yolk sac to form anterior brain regions and a beating heart biorxiv.org
Human-based New Approach Methodologies in Developmental Toxicity Testing: A Step Ahead from the State-of-the-art with a Feto-placental Organ-on-a-chip Platform doi.org
Multiplex profiling of developmental enhancers with quantitative, single-cell expression reporters biorxiv.org
Morphogenesis and development of human telencephalic organoids in the absence and presence of exogenous ECM biorxiv.org
Dissecting peri-implantation development using cultured human embryos and embryo-like assembloids biorxiv.org
Measurement of adhesion and traction of cells at high yield (MATCHY) reveals an energetic ratchet driving nephron condensation biorxiv.org