Multi-compartment immune and tumor cell reprogramming by IFNa2 overcomes colon cancer immunotherapy resistance biorxiv.org 2026-07-01 Tumor cell PD-L1 represses IFN signaling to limit anti-tumor immunity, despite an IFN-responsive immunophenotype predicting colorectal cancer response
ARL15 promotes inflammatory fibroblast activation and disease severity in rheumatoid arthritis: integrated transcriptomic and collagen-induced arthritis model analyses biorxiv.org 2026-07-01 Background ADP-ribosylation factor-like protein 15 (ARL15) is a rheumatoid arthritis (RA) susceptibility gene identified through GWAS.
Viral capsid delivery of cGAMP enhances STING-dependent antitumor immune response biorxiv.org 2026-07-01 Therapies to activate the STING immune response pathway represent promising potential anticancer treatments.
CD56dimCD16dim NK cells are the dominant effector cells against HIV-infected primary T-cells biorxiv.org 2026-07-01 Despite being rare among circulating natural killer (NK) cells and expressing 10-fold less CD16 than the predominant CD56dimCD16bright population, CD5
Nitrogen use efficiency in pigs is associated with transcriptomic signatures related to amino acid metabolism, immune activity, and nutrient partitioning biorxiv.org 2026-07-01 Dietary protein restriction challenges the allocation of amino acids to growth and other physiological functions and therefore requires coordinated me
Deciphering the nucleotide driven kinetic and oligomeric dynamics in IMPDH regulation biorxiv.org 2026-07-01 Inosine monophosphate dehydrogenase (IMPDH) controls guanine nucleotide biosynthesis.
DESI-MS-Based Analysis of Drug Distribution in Human Renal Cystic Tissue Using the Chorioallantoic Membrane (CAM) as a 3D In Vivo Model biorxiv.org 2026-07-01 The chorioallantoic membrane (CAM) model represents a promising three-dimensional in vivo platform for preclinical drug testing in human tissues.
Dietary Sodium Deprivation Remodels the Serum Lipidome and Reveals Systemic Metabolic Adaptation in Rats biorxiv.org 2026-07-01 Background: Dietary sodium restriction is a common nutritional and physiological challenge that activates electrolyte-conserving endocrine pathways, b
Tracking claim changes from preprint to publication across 72,644 biomedical studies using large language models biorxiv.org 2026-07-01 Preprints now disseminate a large share of biomedical research before peer review.
Headbutting goats self-inflict traumatic brain injury biorxiv.org 2026-07-01 Traumatic brain injury (TBI) is a characteristic feature of neurodegenerative diseases such as Alzheimers disease and chronic traumatic encephalopathy
Sequential infection reprograms the immune landscape to shape future responses biorxiv.org 2026-07-01 Mouse models have been instrumental in defining immune mechanisms but often fail to capture the complexity of human immunity, limiting clinical transl
Disruption of a CCR5-like immunoglobulin gene is linked to plague susceptibility in black-footed ferrets biorxiv.org 2026-07-01 Black-footed ferrets (Mustela nigripes) are highly susceptible to sylvatic plague caused by Yersinia pestis, but the genetic basis of this vulnerabili
Mitochondrial STAT3-mediated suppression of apoptosis constrains antimycobacterial immunity biorxiv.org 2026-07-01 Maintenance of mitochondrial homeostasis is required to balance the host-pathogen interface during Mycobacterium tuberculosis (Mtb) infection.
Technical Note: Focused ultrasound-mediated blood-brain barrier opening for delivery of LNP-packaged modRNA therapy in a mouse model of Niemann-Pick Disease Type C biorxiv.org 2026-07-01 Efficient delivery of molecular therapies to the central nervous system (CNS) remains a major barrier to treating neurogenetic disorders such as Niema
Ang2 and TAT targeting of leptomeningeal disease by the intravenous and intrathecal routes: a comparative analysis biorxiv.org 2026-07-01 Leptomeningeal disease (LD), involving the metastasis of cells to the leptomeningeal membranes in the central nervous system (CNS), can be a deadly co
Solvent-free Nanoparticle Assembly Protocol (SNAP): one-pot formulation of drug loaded polyester nanoparticles and their vessel size-dependent perivascular transport biorxiv.org 2026-07-01 This work describes a new approach for rapid and reproducible formulation of drug loaded biodegradable nanoparticles based on polyester copolymers, in
Dynamically actuated reconfigurable topographical surface enables active control of implant-associated infections biorxiv.org 2026-07-01 Implant-associated infections are driven by bacterial biofilm formation and remain difficult to eradicate using conventional antibiotic-based strategi
Spores-on-a-Chip: a new sample-loading strategy for multiplexed single-cell monitoring of spore germination within a microfluidic platform biorxiv.org 2026-07-01 We demonstrate a new sample-loading strategy ('spot-loading') for Spores-on-a-Chip microfluidic sensors that enables multiplexed experimentation.
Dodecagon light-sheet fluorescence microscopy for large-volume imaging without striping artifacts biorxiv.org 2026-07-01 Light-sheet fluorescence microscopy (LSFM) has revolutionized biological imaging by enabling high spatial and temporal resolution with minimal photoda
A dermal-epidermal junction-inclusive skin model enabled by controllable hydrogel swelling biorxiv.org 2026-07-01 Tissue engineered skin models are important tools for the in vitro study of physiological and pathophysiological processes as well as the valuation of
An engineered IdeS variant with enhanced activity and performance for IgG degradation biorxiv.org 2026-07-01 IgG-degrading enzymes have emerged as innovative therapeutic agents for treating conditions driven by pathogenic antibodies.
Penumbria: Advanced 3D cell segmentation for biomedical imaging biorxiv.org 2026-07-01 Quantitative analysis of three-dimensional cellular architecture is fundamental to understanding tissue organization, disease progression, and drug re
BOSE: A Bayesian Order Statistics-Based Estimator for Recovering the Sample Mean and Standard Deviation biorxiv.org 2026-07-01 In meta-analyses of continuous outcomes, the sample mean and standard deviation (SD) are essential for synthesizing effect sizes across studies.
Complex coacervation reshapes the aggregation landscape of tau biorxiv.org 2026-07-01 Biomolecular condensates are increasingly implicated in protein aggregation, yet their contribution is often reduced to that of concentrating reactant
TGM2-mediated histone serotonylation is an epigenetic cardioprotective mechanism in HFpEF biorxiv.org 2026-07-01 Heart failure with preserved ejection fraction (HFpEF) is a heterogeneous syndrome with incompletely understood molecular mechanisms.
Climate change and socioeconomic vulnerability: The Carpathian Basin as a potential hotspot in the dissemination of Dirofilaria repens in Europe biorxiv.org 2026-07-01 Background: Dirofilaria repens is a zoonotic parasite expanding unnoticed across Europe due to climate change.
Genome architecture shapes the evolutionary origins of redundant enhancers in fly and mouse biorxiv.org 2026-07-01 Shadow enhancers are groups of DNA regulatory elements that control the same target gene and drive overlapping expression patterns.
Single molecule footprinting measures low nucleosome occupancy in mature spermatozoa of mice and men biorxiv.org 2026-07-01 Nucleosomes are fundamental units of DNA packaging and gene regulation in eukaryotes.
A conserved architectural domain shapes centromere evolution in Drosophila biorxiv.org 2026-07-01 Centromeres ensure faithful chromosome segregation despite being embedded within rapidly evolving repetitive DNA, a contradiction known as the centrom
Kinetic Lipidomics: Quantifying in vivo changes in lipid metabolism using metabolic labeling biorxiv.org 2026-07-01 Lipid metabolism reflects the dynamic balance between metabolic turnover and concentration.
Data-adaptive three-dimensional deconvolution and evaluation for volumetric fluorescence microscopy biorxiv.org 2026-07-01 Optical fluorescence microscopy enables visualization of biological structures and dynamics.
Intrathecal infusion of hypertonic fluid enables CSF Flow Enhancement (CFE) to facilitate nanoparticle delivery to the brain and spinal cord biorxiv.org 2026-07-01 Intrathecal (IT) drug delivery, i.e., the infusion of substances directly into cerebrospinal fluid (CSF) by lumbar, ventricular, or cisternal access p
A Pubic Hair Is 172 Times More Pubic Than a Scalp Hair biorxiv.org 2026-07-01 Human hair is a common contaminant in GMP-controlled manufacturing environments, and its identification is important for contamination source investig
Body-Axis Reorientation in Regenerating Hydra under Geometric Confinement biorxiv.org 2026-07-01 Defining a body axis is a central aspect of animal morphogenesis.
Evolutionary Origins of Molecular Programs Underlying Brain Circuitry biorxiv.org 2026-07-01 The vertebrate pallium harbors independently evolved structures that, nevertheless, support strikingly similar sensory and cognitive circuit architect
Pathogenic impact of ABCA4 missense variants in the structurally uncharacterized ECD1 region: implications for Stargardt disease. biorxiv.org 2026-07-01 Pathogenic mutations in the ABCA4 gene cause several inherited retinal diseases, particularly Stargardt disease (STGD1).
Evolutionary Stratification of Codon Usage Bias In Plants Arises from GC3 Composition and Translational Optimization biorxiv.org 2026-07-01 Codon usage bias is a fundamental genomic characteristic that prefers non-random preferential use of synonymous codons.
mirCCC: Repression-aware graph learning for miRNA-mediated cell-cell communication inference biorxiv.org 2026-07-01 Cell-cell communication analyses usually focus on protein ligands and receptors and therefore miss the extracellular vesicle-mediated transfer of micr
Accumulated Cytotoxicity Induced by Islet Amyloid Polypeptide Oligomers in Type 2 Diabetes biorxiv.org 2026-07-01 Type 2 diabetes is characterized by progressive aggregation of islet amyloid polypeptide (IAPP) within the islets of Langerhans, a process strongly im
A longitudinal fNIRS hypercanning data set of same generation and intergenerational relationship development and collaboration biorxiv.org 2026-07-01 Social disconnection (i.e., loneliness and social isolation) poses a severe risk to health and is particularly prevalent among older adults.
HIV Infection in Humanized Microglial Mice Disrupts Feeding Behavior and Circadian Rhythms with Cortical Neuroinflammation biorxiv.org 2026-07-01 Although effective antiretroviral therapy (ART) has substantially reduced the severity of human immunodeficiency virus (HIV)-associated neurocognitive
The lung tissue environment in Mycobacterium tuberculosis infection determines local monocyte differentiation biorxiv.org 2026-07-01 Infection by Mycobacterium tuberculosis (Mtb) is characterized by pathogen persistence in lung cells derived from blood monocytes.
LysoDCs in Peyer's patches program compartmentalized microbiota-specific Th17 responses biorxiv.org 2026-07-01 Segmented filamentous bacteria (SFB) are a canonical model of microbiota-driven Th17 immunity, but how distinct gut-associated lymphoid tissues shape
Spike-in-normalised single-cell RNA-seq reveals cell-type-specific transcriptional repression during ageing biorxiv.org 2026-06-30 Transcriptome analyses are widely used for biomarker discovery and to gain insights into normal processes and diseases.
Self-antigen disrupts cDC1 mediated antitumor responses biorxiv.org 2026-06-30 Conventional dendritic cells navigate complex tissues and sample peripheral antigens, balancing immune suppression and activation to achieve tissue ho
Diversifications of both the three domains of life and SARS-CoV-2 possibly driven by biases between amino acid biosynthetic families biorxiv.org 2026-06-30 All cellular life forms fall under the three-domain classification of life, raising a fundamental evolutionary question: why does this classification
CyStainer: A transformer-based variational autoencoder for robust marker imputation in high-parameter cytometry biorxiv.org 2026-06-30 High parameter cytometry is essential for clinical diagnostics through precise immune cell profiling, improved patient stratification, and monitoring,
Age-dependent disease tolerance to SARS-CoV-2 infection biorxiv.org 2026-06-30 Disease tolerance limits infectious disease severity through tissue damage control mechanisms that do not target pathogens directly.
Generation and MHC class II loading of an endogenous influenza epitope revealed by a T cell receptor-like antibody biorxiv.org 2026-06-30 CD4+ T cells coordinate immune responses to infections and cancers and initiate many autoimmune diseases, yet the intracellular pathways that generate
Uncoupling Drug and Microbiota Contributions to Chemotherapy-Induced Gut Toxicity biorxiv.org 2026-06-30 Cytotoxic chemotherapy remains a cornerstone of cancer treatment but is frequently limited by gastrointestinal toxicity associated with epithelial bar