Protein Surface Site Determines the Evolutionary Accessibility of Allosteric Regulation biorxiv.org 2026-07-03 Domain recombination is a major source of new allosteric regulation in both evolved and engineered proteins.
A Minimal Stochastic Model of Microbial Ecological Dynamics in a Single-Species-Single-Resource Setting biorxiv.org 2026-07-03 Microbes exhibit complex dynamic behavior as the result of a large number of biochemical processes, spatial and temporal interactions, environmental v
Supershoes or Superhumans? A controlled analysis of sex-specific road-running performance evolution in the era of advanced footwear technology biorxiv.org 2026-07-03 Advanced footwear technology (AFT) improves running economy and is widely credited with recent performance improvements in road running, with observat
Distinct RNA engagements define genome import and replication elongation in alphaviruses biorxiv.org 2026-07-03 Membrane-associated replication complexes (RCs) are a hallmark of alphaviruses, yet the mechanisms by which viral RNA is delivered into these compartm
Clade 2.3.4.4b H5N1 influenza virus and SARS-CoV-2 seroprevalence among owned and feral cats in Philadelphia and surrounding communities biorxiv.org 2026-07-03 Clade 2.3.4.4b H5NX influenza viruses have spread widely in birds since 2020.
Dysregulation of pupal diapause in hybrid progenies, introgression and species delimitation within and beyond the Old World Swallowtail (Papilio machaon Linnaeus) butterfly complex biorxiv.org 2026-07-03 In search of recurrent patterns of postzygotic hybrid incompatibility, we investigated the Holarctic 'Old World Swallowtail' (Papilio machaon Linnaeus
Heat stress drives opposing redox shifts in temperate versus tropical Drosophila melanogaster embryos biorxiv.org 2026-07-03 Redox balance is central to aerobic metabolism, yet acute heat stress can destabilize this balance by increasing metabolic rates and shifting the bala
Evolution of mutation rates in digital genomes: the roles of genetic drift, mutational supply, and genome size biorxiv.org 2026-07-03 Mutation is the ultimate mechanism that produces genetic novelty, and thus a central ingredient of evolution.
Asymmetric migration shapes genetic structure of the invasive avian vampire fly (Philornis downsi) across the Galapagos Islands. biorxiv.org 2026-07-03 Biological invasions on islands provide a natural framework to study how dispersal and connectivity influence evolutionary and ecological processes.
Climate-driven fitness decline in Japanese chum salmon reshapes North Pacific chum salmon biogeography biorxiv.org 2026-07-03 Japanese chum salmon supported by one of the world largest hatchery programs have experienced severe declines in marine survival and egg size.
Ecological genomics of a novel host-parasitoid arms-race in nature biorxiv.org 2026-07-03 Novel antagonistic interactions between species are expected to drive especially rapid coevolution.
Mobile DNA Activity in Parkinson's Disease: A Locus-Specific View of Endogenous Retroviruses biorxiv.org 2026-07-03 Human endogenous retroviruses (HERVs) are mobile genetic sequences derived from ancient retroviral infections.
THE STRUCTURES OF MATURE AND IMMATURE ST. LOUIS ENCEPHALITIS VIRUS REVEAL CONSERVED HISTIDINE RESIDUES AFFECTING VIRUS FITNESS biorxiv.org 2026-07-03 Orthoflaviviruses undergo significant structural changes through maturation and infection, yet the molecular mechanisms remain incompletely understood
Replication fork directionality reveals how structural variants arise under replication stress biorxiv.org 2026-07-03 Structural variants (SVs) in cancer are associated with defects in DNA repair and replication stress, but the mechanisms generating common SV types re
Crowding on DNA modulates SSB protein binding mode kinetics biorxiv.org 2026-07-03 Single-stranded DNA-binding (SSB) proteins play a crucial role in DNA replication by binding to single-stranded DNA (ssDNA) in multiple binding modes,
A Comprehensive Evaluation of Protein Structure Prediction Models for Short Peptides biorxiv.org 2026-07-03 Short peptides pose distinct challenges for computational structural biology due to their lack of stable tertiary structures, high conformational flex
Nondual mindfulness meditation alters self representation and brain connectome in expert meditators biorxiv.org 2026-07-03 Nonduality is a foundational but conceptually elusive notion across several contemplative traditions.
Loss of HIF1α signaling drives oxidative stress and expansion of smooth muscle cells in murine atherosclerosis biorxiv.org 2026-07-03 Background: Hypoxia develops within growing atherosclerotic lesions, inducing nuclear translocation of hypoxia-inducible factor-1 (HIF1) and metabolic
Analysis of genetic variation in the bovine Mannose Receptor gene (MRC1), its influence on receptor expression, and a potential association with resistance to bovine tuberculosis biorxiv.org 2026-07-03 Naturally occurring variation in the bovine mannose receptor C-type 1 gene (MRC1) may shape macrophage responses to Mycobacterium (M.) bovis, a key dr
Carboxypeptidase activity drives L,D-transpeptidase essentiality during vegetative growth and sporulation in Clostridioides difficile biorxiv.org 2026-07-03 In most bacteria, peptidoglycan contains mainly 4-3 crosslinks formed by penicillin-binding proteins (PBPs).
Time-of-flight-resolved interferometric speckle-contrast optical spectroscopy (TOF-iSCOS) for depth-resolved blood-flow sensing biorxiv.org 2026-07-03 Significance: Continuous, noninvasive, and depth-resolved monitoring of blood-flow-related tissue dynamics remains an important unmet need.
Preserved Barrier Integrity and Altered Immune Profiles in Chronic Cannabis Users: Potential Roles of Δ9-Tetrahydrocannabinol biorxiv.org 2026-07-03 Although cannabinoids such as delta-9-tetrahydrocannabinol (THC) are generally immunosuppressive in preclinical models, chronic cannabis use in humans
The illusion of diversity: sampling design drives conflicting estimates of soil bacterial richness biorxiv.org 2026-07-03 Soil microbial diversity is shaped by the spatial scale at which communities are sampled, yet standard sampling practices often homogenize samples, ob
The Hunt for Cholesteryl Ester Hydrolases: Identification of Lipoprotein Lipase as a Cholesterylesterase biorxiv.org 2026-07-03 Cholesteryl esters (CEs) are central intermediates in cholesterol storage and transport, yet the enzymes responsible for their hydrolysis in mammals r
Analyte-Class-Dependent Electrophoretic Organization Enables Single-Run Proteome-Metabolome Analysis by CE-ESI-MS biorxiv.org 2026-07-03 Dual proteome-metabolome measurements from limited samples typically require sample splitting or sequential analyses using electrospray ionization mas
Internalized Components of Membrane Attack Complexes Disrupt Proteostasis and Acquire Alarmin-Like Properties biorxiv.org 2026-07-03 Immune effects of membrane attack complexes (MAC) have been widely attributed to their abilities to cause cell death.
Unveiling Cerebrospinal Fluid Protein Biomarkers in Pediatric Acute Lymphoblastic Leukemia Using Proximity Extension Assay biorxiv.org 2026-07-03 Abstract Background: Acute Lymphoblastic Leukemia (ALL) is a highly heterogeneous pediatric malignancy.
Conservation and evolution of the ribosome across the photosynthetic lineage biorxiv.org 2026-07-03 Regulated biogenesis of photosynthetic proteins by the chloroplast ribosomes is central to plant development and environmental adaptation.
Contextualised real-time mass spectrometry improves glycosylation detection and characterisation biorxiv.org 2026-07-03 Glycosylation is a structurally diverse, non-template-driven modification whose analysis by liquid chromatography-mass spectrometry is constrained by
L-5--glutamine PET of Breast Cancer: Kinetic Analysis in Mouse Models to Evaluate Glutamine Metabolism biorxiv.org 2026-07-03 Abstract (250 words) Background: Glutamine addiction is a hallmark of aggressive tumors, yet glutaminase (GLS1) inhibitor CB-839 showed disappointing
Adenosine A2B Receptor Activation: A Novel Therapeutic Strategy for Accelerating Liver Recovery After Acetaminophen Overdose biorxiv.org 2026-07-03 An acetaminophen (APAP) overdose is the leading cause of drug-induced hepatotoxicity and acute liver failure (ALF) in the United States.
Plasma metabolite responses to an oral protein tolerance test differ between young and sarcopenic participants and suggest altered anabolic sensitivity biorxiv.org 2026-07-03 Abstract Background: Sarcopenia is associated with anabolic resistance, a blunted muscle protein synthesis response to protein ingestion.
Charge-trap flash memory cells of the brain biorxiv.org 2026-07-03 Despite extensive study of cellular mechanisms underlying long-term potentiation, no single specific protein or gene has been identified which encodes
Aging reveals domain-specific vulnerability to the chronic behavioral consequences of repetitive mild traumatic brain injury biorxiv.org 2026-07-03 Older adults are among the fastest growing groups of traumatic brain injury (TBI) patients and sustain disproportionately poor chronic outcomes.
Somatosensory gating dysfunction is masked by cognitive variability in cognitively impaired individuals biorxiv.org 2026-07-03 Disruptions in somatosensory processing have been observed in cognitive impairment (CI), suggesting that alterations in sensory processing may emerge
Eyes on the prize: Mice deploy task-driven saccades during naturalistic foraging biorxiv.org 2026-07-03 Active sensing allows organisms to shape incoming sensory information through self-generated actions, and saccadic eye movements provide a key readout
200 years of river engineering: changes in meta-ecosystem resilience and connectivity biorxiv.org 2026-07-03 This study investigates changes in habitat connectivity and meta-ecosystem resilience between 1817 and 2022 along a 150 km section of a large European
Pangenome-based human genome analysis improves trait association and genomic prediction biorxiv.org 2026-07-03 The Human Pangenome Reference Consortium has generated 462 open-access reference genomes and a variation graph that represents differences among them,
Selective and Efficient Functionalization of P22 Virus-Like Particles Using an Asparaginyl Ligase biorxiv.org 2026-07-03 Protein cages provide useful scaffolds for nanoscale engineering due to their highly ordered structures and in vivo self-assembly.
RD-OMICS: An Integrative Multi-Omics Data Inventory in Rare Diseases biorxiv.org 2026-07-03 Rare diseases (RD) impact over 30 million individuals in the United States, yet fewer than 5% of the identified conditions have FDA-approved treatment
Structural Organization of the Nvj3-Mdm1 Complex Reveals a Conserved Lipid-Compatible Contact Site Module biorxiv.org 2026-07-03 Membrane contact sites are organized by protein assemblies that physically couple organelles and coordinate lipid metabolism, yet the structural princ
RegulomeXplorer: Interactive exploration of drug effects on subcellularly resolved proteomes biorxiv.org 2026-07-03 Mass spectrometry-based proteomics allows the quantification of drug-induced changes in protein abundance.
Age and Social Observation Effects on Theta Synchrony and Its Role in Adolescent Post-Error Control: A Computational Approach biorxiv.org 2026-07-03 Error monitoring allows for detecting mistakes and adapting behavior.
Contrasting Mitochondrial Diversity of Endemic Corbicula Clams in Sulawesis Ancient Lakes: Phylogeography and Implications for Conservation biorxiv.org 2026-07-03 The global study of freshwater clams in the genus Corbicula is frequently confounded by invasive androgenetic lineages that experience mitochondrial D
Towards genetic indicators in ectomycorrhizal fungi: estimating the effective population size biorxiv.org 2026-07-03 Ectomycorrhizal (EM) fungi are vital to forest ecosystems, supporting tree growth and survival.
Ultra-accurate sequencing reveals an extreme transmission bottleneck in a deep-sea clam symbiosis biorxiv.org 2026-07-03 Vertically transmitted symbionts experience progressive genome degradation driven by transmission bottlenecks each host generation that reduce genetic
Comparative genomics reveals shared accessory regions between members of two Fusarium species complexes virulent on garden pea biorxiv.org 2026-07-03 The contribution of accessory or conditionally dispensable chromosomes to host-specific virulence was first demonstrated in members of the Fusarium so
Evo 2's Perception of Single Nucleotide Substitutions in the Genes of Two Plant Model Organisms biorxiv.org 2026-07-03 Although DNA Large Language Models (DNA-LLMs) offer a path to decoding genetic complexity, our ability to evaluate these models is constrained by our
Genome-Wide Markers Predict Metribuzin Tolerance in Southern Soft Red Winter Wheat biorxiv.org 2026-07-03 Metribuzin is a versatile herbicide effective against various annual grasses and broadleaf weeds found in wheat fields.
Song sparrows fail to discriminate between current and historical songs biorxiv.org 2026-07-03 Song evolves rapidly in songbirds, as has been proven for a substantial number of songbird species by demonstrating acoustic differences between curre