SSUplex: fast, both-strand extraction and origin-sorting of small-subunit rRNA for environmental DNA metabarcoding biorxiv.org 2026-07-05 Ribosomal RNA metabarcoding sits at the centre of how we characterise microbial and eukaryotic communities in environmental samples, and long-read seq
A National Survey of 205 Canadian Biomedical Research Core Facilities Reveals Structural Challenges and Opportunities for Strengthening Canada's Research Ecosystem biorxiv.org 2026-07-05 Background: Canadian biomedical core facilities (BCFs) provide researchers with access to advanced tools and unique technical expertise, essential for
Individualized parcellation reveals functional boundaries in human prefrontal cortex biorxiv.org 2026-07-06 Human prefrontal cortex (PFC) supports diverse higher-order cognitive functions during task execution.
Presynaptic Terminals Dynamically Modulate Spontaneous Release Frequency During Early Synaptic Plasticity Through and Entropic Force Framework biorxiv.org 2026-07-06 Spontaneous synaptic transmission has been established as essential for the maintenance of synaptic weights during action potential-induced transmissi
Astrocyte-to-microglia purinergic signaling mediates synaptic shielding and promotes neuronal activity biorxiv.org 2026-07-06 How glial cells sense and regulate neuronal hypoactivity remains poorly understood.
Neonatal oxytocin prevents sex-specific spatial memory deficits induced by maternal separation through restoration of hippocampal synaptic plasticity in males biorxiv.org 2026-07-05 Stress during critical developmental periods causes lasting neurobiological alterations.
Behavior Differentially Shapes Spontaneous Cortical Network Dynamics Across Frequencies biorxiv.org 2026-07-06 The rapid and coordinated propagation of neural activity across a network of distributed brain regions underpins complex behavior and cognition.
A comparison of sharpening and dampening accounts of the role of expectation in shaping the neural fidelity of early visual representations biorxiv.org 2026-07-06 The natural environment is spatiotemporally structured, and the brain exploits this regularity to predict and prepare for upcoming sensory stimuli.
Striatal Dopamine at Learned Sequence Boundaries Sustains Birdsong biorxiv.org 2026-07-06 Phasic striatal dopamine has been implicated in the initiation of well-trained action sequences in reward-guided tasks.
Sphingosine 1-phosphate lyase expressed in pulmonary epithelial cells potentiates host innate defenses and alleviates influenza pathogenicity in mice biorxiv.org 2026-07-05 Influenza viruses circulate in humans, causing a substantial burden on global health.
A High Throughput SPR-Based Array for Quantitative Profiling of Glycosaminoglycan Protein Interactions biorxiv.org 2026-07-04 Glycosaminoglycans (GAGs) are linear, negatively charged, polysaccharides that mediate a wide variety of biologically critical interactions with prote
A detailed molecular picture of protein folding during active translation biorxiv.org 2026-07-04 All proteins can begin to fold on the ribosome, and many proteins critically rely on co-translational folding to attain their native conformation.
A structural trade-off balances mechanical resilience and supramolecular adaptability in type IV pili biorxiv.org 2026-07-04 Biological machines operate under mechanical load, requiring architectures that simultaneously resist force and remain functionally dynamic.
A Bottom-Up Platform for Quantitative Single-Molecule Tracking Through Bacterial Biofilm Mimics biorxiv.org 2026-07-04 Chronic infections persist in large part thanks to protection that biofilms afford their bacterial creators.
Cross-protocol comparison of iPSC-microglia reveals hypofunction contributes to neuronal vulnerability and synaptic alterations in the MAPT-S305N model of frontotemporal dementia. biorxiv.org 2026-07-04 Progressive and chronic neuroinflammation is associated with numerous neurodegenerative diseases, including primary tauopathies such as frontotemporal
Connectome-scale self-supervised representation learning reveals neuronal organization beyond canonical labels biorxiv.org 2026-07-04 Dense electron-microscopy connectomes provide synaptic-resolution maps of neuronal structure and wiring, but learning scalable representations that in
Neural representation of hedonic valence during narrative listening biorxiv.org 2026-07-04 Hedonic valence, the intrinsic pleasantness or unpleasantness of an experience, is fundamental to human psychological functioning.
Microbial induction of MHC-II expression in colon cancer cells overcomes immunotherapy resistance and limits metastasis biorxiv.org 2026-07-04 Colorectal cancer remains a major cause of cancer mortality, and most microsatellite stable tumors derive little benefit from immune checkpoint blocka
PRISM : Peptide-specificity annotation of T-cell receptors with uncertainty quantification biorxiv.org 2026-07-04 Mapping T-cell receptor (TCR) sequences to their cognate peptide-major histocompatibility complex (pMHC) ligands underlies both basic immunology and T
Colorectal cancers with distinct metastatic potential trigger divergent early T cell responses biorxiv.org 2026-07-04 Colorectal cancer (CRC) remains a leading cause of cancer mortality, with most cases refractory to immunotherapy.
Bacillus adaptation to Pseudomonas secondary metabolites enhances its root competitiveness biorxiv.org 2026-07-04 Bacillus velezensis is a widely used plant growth-promoting rhizobacterium whose effectiveness under natural conditions is strongly influenced by inte
From planktonic to sedentary lifestyle: Molecular dissection of the establishment and maintenance of mycobacterial biofilm biorxiv.org 2026-07-04 Biofilm represents a complex aggregation of bacteria embedded within a self-produced extracellular polymeric substance (EPS).
HLiCA: An integrated cell atlas of the healthy human liver biorxiv.org 2026-07-04 The human liver is composed of a heterogeneous mix of cell types.
SPICE: A Robust Computational Framework for Identifying Copy Number Variations in Spatial Transcriptomics biorxiv.org 2026-07-04 Copy number variation (CNV), which alters the number of genomic segments, is a major driver of intratumor heterogeneity, characterized by spatially or
Convergent Cysteine Enrichment in Diverse Gut Phage Capsids Suggests Gut-Associated Structural Adaptation biorxiv.org 2026-07-04 Background: The gut environment is hostile to life, yet the human virome, dominated by bacteriophages, persists.
CryoROLE: describing large inter-domain rotation in single particle cryo-EM biorxiv.org 2026-07-04 In single particle cryo-EM, analysis of continuous conformational heterogeneity has always been challenging.
The influence of virtual visual stimulus amplitude to induce standing postural responses biorxiv.org 2026-07-04 Upright postural control during movement relies on multisensory integration.
Multimodal molecular profiling of the metabolic penumbra in hyperacute stroke biorxiv.org 2026-07-04 Background The ischemic penumbra, a metabolically compromised yet potentially salvageable region surrounding the ischemic core, is a prime target for
Microbiome-derived Short Chain Fatty Acids modulate microglial inflammatory responses in a sex- and metabolite-specific manner biorxiv.org 2026-07-04 Microbes residing in the gastrointestinal tract exert immunomodulatory impacts on the brain through the gut-brain axis.
Single-Molecule Imaging Reveals Differential Stability of Alpha-Synuclein Aggregates biorxiv.org 2026-07-04 Alpha-Synuclein (-syn) aggregation is central to Parkinson's disease (PD), yet measurements in biofluids are confounded by the coexistence of monomeri
Connectome quality converges predictably to reveal optimal stopping points during proofreading biorxiv.org 2026-07-04 Volumetric electron microscopy (EM) has become a critical approach to generating high-resolution reconstructions of brain tissue.
Local inhibitory topology dictates the spatial compartmentalization of hippocampal sharp-wave ripples biorxiv.org 2026-07-04 Hippocampal sharp-wave ripples (SWRs) are essential for memory consolidation and represent among the most synchronous oscillatory events in the brain.
Peptide allosteric inhibitor of TNFR1 signaling attenuates inflammation and rheumatoid arthritis pathology in human TNF transgenic mice biorxiv.org 2026-07-04 Inhibition of tumor necrosis factor receptor 1 (TNFR1) represents a major therapeutic strategy for chronic autoimmune and inflammatory diseases such a
TRIOPS: A deep learning framework for prediction of T cell receptor-MHC binding specificity biorxiv.org 2026-07-04 T cell receptor (TCR) recognition is MHC-restricted, yet accurately predicting a TCR's restricting HLA allele remains an open problem.
The role of stridulations during the mating of Nicrophorus vespilloides biorxiv.org 2026-07-04 In many insect species, mating is coordinated through multimodal signaling, yet less obvious channels are often overlooked.
Differential effects of piroxicam and nitroglycerine on memory and hippocampal neurochemistry in di-oestrous female rats biorxiv.org 2026-07-04 Abstract Objectives To evaluate and compare the neuro-behavioural safety profiles of piroxicam and nitroglycerine by investigating their differential
Structural brain alterations in autism: A large-scale voxel-based morphometry mega-analysis biorxiv.org 2026-07-04 Background: Previous large-scale structural MRI analyses of the brain in autism have identified gray matter (GM) differences when using region-of-inte
Spatiotemporal transformation of neural data reveals representations of erroneous behaviors biorxiv.org 2026-07-04 Abnormal states such as erroneous behaviors are generally difficult to represent from neural data.
Oxytocin receptor dysfunction during neurodevelopment programs lasting pain hypersensitivity and sex-specific cognitive deficits biorxiv.org 2026-07-04 Early life stress (ELS), modeled in rodents through neonatal maternal separation (NMS), induces lasting behavioral and molecular alterations including
Direct comparison of CRISPR knockout and interference with Perturb-seq biorxiv.org 2026-07-04 CRISPR knockout (CRISPRko) and CRISPR interference (CRISPRi) are two workhorse technologies for loss-of-function studies, yet direct comparisons betwe
Orbitrap Collision Cross Section Measurements Enhance Isomer Annotations in Lipidomics biorxiv.org 2026-07-04 Despite advances in high-resolution mass spectrometry (HRMS), confident lipid annotation remains challenging due to the extensive chemical diversity o
Unbalanced Perturbation Dynamics For Cell Fate Design biorxiv.org 2026-07-04 Large-scale single-cell perturbation sequencing provides an unprecedented opportunity to construct virtual cells for the in silico simulation of cellu
A High-Confidence Atlas of Protein Methylation Enables AI-Driven Detection of Methylated Peptides biorxiv.org 2026-07-04 Lysine and arginine methylation regulate chromatin dynamics, transcription, and cellular signaling, however confident mass spectrometry (MS)-based det
Saturation-seq integrates single-cell saturation genome editing and RNA-seq to quantify NFE2L2 (NRF2) variant effects biorxiv.org 2026-07-04 Interpreting the functional consequences of variants remains one of the central unsolved problems in genomics and clinical genetics.
Accurate, comprehensive gene annotation and ortholog identification across thousands of vertebrate genomes with TOGA2 biorxiv.org 2026-07-04 Inferring orthologs and annotating coding genes remain central challenges in genomics, evident by the growing gap between assembled and annotated geno
Cohesin residence time gates 3D genome response to histone hyperacetylation biorxiv.org 2026-07-04 Cohesin-mediated loop extrusion and chromatin state-dependent compartmentalization are major drivers of three-dimensional (3D) genome organization.
A cellular midbrain mechanism for executing fast and reliable escape biorxiv.org 2026-07-04 Escape from threat is one of the most ancient and conserved sensorimotor transformations and must satisfy two competing demands.
Chemogenetic inhibition of the noradrenergic locus coeruleus promotes the development of risk-taking decisional strategies and selectively enhances motor impulsivity in females biorxiv.org 2026-07-04 The locus coeruleus noradrenaline (LC-NA) system is a key regulator of arousal, attention, and reward learning.
Performance of pathogen identification and resistance gene expression tests using ASTar(R) remnant bacterial suspension in Gram-negative contrived positive blood cultures biorxiv.org 2026-07-04 Introduction: Rapid pathogen identification, resistance detection, and susceptibility profiling improve antimicrobial prescribing and associated outco
HERC4 limits oxidative stress-induced DNA damage during bacterial and viral-bacterial infection biorxiv.org 2026-07-04 Respiratory (co-)infections caused by influenza viruses and Streptococcus pneumoniae represent significant threats to global health.