High-density Lipoprotein Inspired Lipid Nanoparticles for Systemic RNA delivery to the Brain biorxiv.org 2026-06-01 Systemic delivery of nucleic acids to the central nervous system (CNS) remains fundamentally limited by the blood-brain barrier (BBB) and the lack of
Monju: Multi-criteria clustering in single-cell omics biorxiv.org 2026-06-01 Clustering is a fundamental step in single-cell omics analysis.
Do AI Structure Predictors Capture Bound-State Disorder? A Benchmark on Fuzzy Protein Complexes biorxiv.org 2026-06-01 Fuzzy protein complexes, in which an intrinsically disordered protein (IDP) retains conformational disorder upon binding, pose a fundamental challenge
DropSynth-Gold: Golden Gate Assembly in Emulsions Extends Multiplexed Gene Libraries to Greater Lengths biorxiv.org 2026-06-01 The ability to synthesize longer genes at scale remains a central challenge in multiplexed gene synthesis.
Integrated valorisation of PET and xylose using the oleaginous microorganisms Yarrowia lipolytica and Rhodococcus jostii biorxiv.org 2026-06-01 The increasing accumulation of polyethylene terephthalate (PET) waste has prompted the development of sustainable biotechnological strategies for its
Vinculin organises apical Transcellular Actin Fibres to coordinate collective migration biorxiv.org 2026-06-01 Collective cell migration is coordinated by adherens junctions (AJs) which serve both as structural links between the actin cytoskeleton of adjacent c
Co-delivered PD-L1 rescues the protective efficacy mediated by an AAV-expressed HIV-1 bNAb biorxiv.org 2026-06-01 Adeno-associated virus (AAV)-delivered anti-HIV-1 broadly neutralizing antibodies (bNAbs) could prevent and treat HIV-1 infection but are limited by h
Fusion of a non-specific DNA-binding domain enhances Cas12a trans-cleavage for robust nucleic-acid diagnostics biorxiv.org 2026-06-01 CRISPR-Cas12a underlies powerful genome-editing and nucleic-acid detection technologies, yet its performance is limited by inefficient target engageme
Data gaps of international databases on HPAI H5 in wildlife in the Americas: implications for surveillance, research, and conservation biorxiv.org 2026-06-01 Global efforts to prevent and mitigate the impacts of high pathogenicity avian influenza (HPAI) H5 on domestic animals, humans, and wildlife rely on t
Song evolution in light of ecosystem differences: exploring effects of urbanization and ecology on temporal and frequency traits of Spotted and Eastern towhee songs biorxiv.org 2026-06-02 The Eastern towhee (Pipilo erythrophthalmus) and Spotted towhee (Pipilo maculatus) are large New World sparrows found across North America.
The role of falx cerebri in the selective vulnerability of splenium within the corpus callosum biorxiv.org 2026-06-02 The corpus callosum is the largest white matter structure connecting the two cerebral hemispheres and is anatomically divided into three major subregi
Prenatal assembly of functional cortical circuits biorxiv.org 2026-06-02 The extent to which early brain maturation requires interactions with the outside world is a central question in neurobiology.
Stimulus-response correlation analysis dissociates spatiotemporal cortical networks supporting speech production biorxiv.org 2026-06-02 Introduction: Understanding the spatiotemporal distribution of cortical activation during language production is a central question in cognitive neuro
Recursive exploration of metabolic yield space biorxiv.org 2026-06-01 Genome-scale metabolic network reconstructions contain extremely detailed and valuable information regarding cellular metabolism.
Reproducible and shareable bioinformatics pipelines from natural-language prompts biorxiv.org 2026-06-01 Large language models (LLMs) are increasingly used to generate bioinformatics pipelines and to carry out analyses from natural-language prompts.
Stochastic Differential Equations (SDEs) in NONMEM for Probing Population Pharmacokinetic Model Misspecification: Diagnostic Utility, Practical Considerations, and Future Directions biorxiv.org 2026-06-01 Population pharmacokinetic (popPK) models are commonly developed using ordinary differential equations (ODEs) to describe deterministic concentration-
Forecasting novel therapeutic development in biomedical research biorxiv.org 2026-06-01 Early identification of promising drug research topics is challenging yet crucial for the scientific community to accelerate the development of novel
Sustained Improvements in Student Outcomes Following Integration of Clinical Case Narratives in Veterinary Microbiology Curriculum biorxiv.org 2026-06-01 Microbiology education in veterinary curricula requires students to integrate complex foundational knowledge with clinical application, yet traditiona
Development positions malignant cellular states but does notexplain their diversification biorxiv.org 2026-06-01 Epithelial cancers are often described as aberrant reactivations of embryonic or tissue-forming programs, but whether malignant cellular-state diversi
A noradrenergic Ih-dependent pacemaker system drives sniffing biorxiv.org 2026-06-01 Sniffs are rapid breaths generated to clear nasal passages, actively sample air for smelling, and, in infants, rouse from sleep.
Proximity labelling of D1-like dopamine receptors reveals distinct cellular environments and uncovers trafficking proteins that regulate DA mediated behaviors in Drosophila biorxiv.org 2026-06-01 The neurotransmitter dopamine (DA) is central to synaptic regulation that support diverse behavioral functions, including both learning and forgetting
HCN channels enhance synchrony propagation in heterogeneous synfire chains biorxiv.org 2026-06-01 Motivation: Information flow and temporal coding in cortical circuits depend critically on the reliable transmission of precisely timed synchronous sp
Redundant γc cytokines license IL-1-driven neutrophil inflammation through MEK/ERK convergence biorxiv.org 2026-06-01 Interleukin 1 (IL1) is a central driver of autoinflammatory disease, yet IL1 blockade often provides incomplete benefit in complex, neutrophil-driven
An Integrating Sphere Based Method for Quantifying RNA Encapsulation Efficiency in Lipid Nanoparticles without Lysis biorxiv.org 2026-06-01 Encapsulation efficiency (EE) is a critical quality attribute of lipid nanoparticle (LNP)-RNA formulations, determining the fraction of active RNA pay
SRC Knockdown Impairs Proliferation, Migration, and Invasion While Promoting Apoptosis in HTR8/SVneo Trophoblast Cells via Activation of the PI3K/Akt/Bcl-2 Signaling Pathway biorxiv.org 2026-06-01 SRC knockdown inhibits trophoblast cell proliferation, migration, and invasion while inducing apoptosis via activation of the PI3K/Akt/Bcl-2 signaling
Deviations from an individual's average sociality have fitness consequences biorxiv.org 2026-06-01 Social relationships are consequential and affect survival, longevity and reproductive success in many species.
Integrated multi-omics reveals adaptive anti-oxidant remodeling in early alcohol-associated liver disease biorxiv.org 2026-06-01 Alcohol-associated liver disease (ALD) is a leading cause of liver-related morbidity and mortality.
Instant Prior-Free Resolution Enhancement for Cross-Modality Microscopy biorxiv.org 2026-06-01 The resolving power of optical microscopy is fundamentally constrained by the diffraction of light, limiting our ability to visualize subcellular stru
GeneKnow: AI-powered literature synthesis for gene-context analysis biorxiv.org 2026-06-01 Interpreting gene function in specific biological contexts is essential for biomedical research, yet manual literature review is labor-intensive.
Palmitoylated importin α recruits PKCε to the plasma membrane to drive breast cancer cell motility biorxiv.org 2026-06-01 Importin is a nuclear transport factor which canonically has a role in binding and shuttling NLS-containing proteins from the cytoplasm into the nucle
Adenosine A2A and A2B receptor signaling in neurons promotes glucose and fatty acid release in the postprandial state biorxiv.org 2026-06-01 Adenosine is a widely distributed signaling molecule whose levels rise during conditions of metabolic stress, hypoxia, or inflammation.
Static allometry of the horn and pronotal depression in adult Oryctes rhinoceros supports continuous nonlinearity, sexual dimorphism, and size-independent covariation biorxiv.org 2026-06-01 Exaggerated insect traits often show positive allometry, yet nonlinear scaling can reflect either continuous curvature or discrete morphs.
Decoding Cognitive States from fMRI Using Classical Machine Learning and Temporal Dynamics Analysis: An Interpretable Approach Using the Human Connectome Project biorxiv.org 2026-06-01 We propose a rigorous and reproducible methodology for analyzing functional MRI data, aimed at: (1) demonstrate their efficiency in classifying task-i
Generalisation of training-induced recovery in occipital stroke: neurochemical and fMRI correlates biorxiv.org 2026-06-01 BACKGROUND: Damage to the early visual cortex after an occipital stroke typically results in the loss of conscious vision in the contralateral hemifie
Jump-starting the T cell response in established tumors biorxiv.org 2026-06-01 Checkpoint blockade only works in 10-20% of patients.
Altered Tonsillar Microbiome in Children with Down Syndrome and Obstructive Sleep Apnea biorxiv.org 2026-06-01 Background and Objectives: Children with Down syndrome (DS) have a high prevalence of obstructive sleep apnea (OSA) due to anatomic, neuromuscular, im
Genetic control of mitotic-to-meiotic transition regulates germline cell survival biorxiv.org 2026-06-01 The coordination between DNA damage repair and cell cycle progression is essential to ensure cell survival and organ homeostasis.
Rediscovery of the eucerine bee Xenoglossa cressoniana in Texas biorxiv.org 2026-06-01 Xenoglossa cressoniana, also known as Tetraloniella cressoniana or Xenoglossodes cressoniana, is a eucerine bee known mainly from the US Great Plains.
Schwann Cell Mapping and Characterization in Bone of Different Embryonic Origins biorxiv.org 2026-06-01 Schwann cells (SCs) provide support for nerves throughout the body.
Species- and Topic-aware Representation Learning for Antimicrobial Peptide Discovery biorxiv.org 2026-06-01 Antimicrobial resistance poses a major global health challenge, necessitating efficient strategies to discover potent antimicrobial peptides (AMPs).
Cost-Effective Purification of Endotoxin-Free LIF, IL-2, and IL-33 biorxiv.org 2026-06-01 We describe a cost-effective Escherichia coli (E. coli)-based platform for producing endotoxin-free cytokines.
Uncovering phenotypic diversity and cell-type-specific prefrontal cortex calcium dynamics in rat fentanyl self-administration biorxiv.org 2026-06-01 BACKGROUND: The proliferation of the potent synthetic opioid fentanyl has exacerbated the ongoing crisis of substance use disorder and associated over
Preclinical Safety Evaluation of Human Lactoferrin Alpha (Effera(R)) biorxiv.org 2026-06-01 The preclinical safety of human-equivalent lactoferrin alpha (heqLF; effera(R)), produced by Komagataella phaffii, was evaluated to support its use as
Nano- and microplastics in pediatric tonsil tissue: bioaccumulation, distribution, and immunomodulatory effects in human lymphoid aggregate organoids biorxiv.org 2026-06-01 Nano- and microplastics (NMPs), by-products of the fragmentation and degradation of plastic products, are ubiquitous environmental contaminants, yet t
Prevotella stercorea links gut microbiome ecology to respiratory infection protection through a host-context-dependent, species-autonomous pathway biorxiv.org 2026-06-01 Using a longitudinal cohort of 633 Gambian children (IHAT-GUT, NCT02941081), we resolve two mechanistically distinct ecological pathways linking Prevo
Selective Activation of Nerve Fiber Subpopulations with Intrafascicular Stimulation biorxiv.org 2026-06-01 Background: Peripheral nerve stimulation (PNS) is most effective when specific nerve fiber subpopulations are activated, while minimizing off-target a
Assessing and Optimizing Low-Frequency Somatic Mutation Detection: A Multi-Platform High-Throughput Sequencing Perspective biorxiv.org 2026-06-01 The availability of multiple commercial short-read sequencing platforms necessitates systematic cross-platform performance comparisons, particularly f
A Multi-Epitope Vaccine Design for Human Pasteurellosis using Outer Membrane β-barrel Proteins of Pasteurella multocida biorxiv.org 2026-06-01 Pasteurella multocida is a facultative anaerobic, Gram-negative coccobacillus that causes pasteurellosis in companion animals (cats and dogs), livesto
Trustworthy ML/AI for Aging Clocks: Preventing Systematic Prediction Bias in Biological Age Estimation biorxiv.org 2026-06-01 Machine learning (ML)- and artificial intelligence (AI)-based aging clocks are increasingly used to quantify physiological and molecular aging from om
Controlled delivery of iNOS antagonist, 1400W, for synergistic breast cancer therapy biorxiv.org 2026-06-01 Triple-negative breast cancer (TNBC) is an aggressive subtype of breast cancer that lacks effective targeted therapies and is frequently associated wi