Efficient ageing: Simulated lesion of the structural connectome reveals optimised decline in the healthy ageing brain biorxiv.org 2026-06-02 Healthy ageing is associated with widespread white-matter change and altered connectome organisation, yet the link between local microstructural decli
Information-dependent eye-hand coordination emerges from active vision biorxiv.org 2026-06-02 In daily activities, humans rely on visual information to plan hand movements, making the extraction of task-relevant information through eye gaze a k
Atomic layer deposition for core-shell microparticle vaccines enabling programmable antigen delivery to lymph nodes enhance humoral immune responses biorxiv.org 2026-06-02 Technologies that simplify complex dosing regimens as single-shot immunizations may be important for vaccines against difficult to neutralize pathogen
Fat body driver expression report across Drosophila melanogaster tissues and sex biorxiv.org 2026-06-02 Drosophila melanogaster serves as a valuable model system for advancing our understanding of adipose tissue given its analogous organ systems, conserv
A Human Vocal Fold Organ-On-Chip for Studying Platform-Dependent Mucosal Responses to Particulate Matter biorxiv.org 2026-06-02 Background. Coarse particulate matter (PM10) deposits at the vocal fold (VF) mucosa, yet upper airway responses remain poorly characterized.
Nutrient-responsive and DAF-16/FoxO target H1 histone HIL-1 promotes resistance to starvation and bacterial pathogens in Caenorhabditis elegans biorxiv.org 2026-06-02 Insulin/IGF-1 signaling (IIS) mediates metabolic and developmental acclimation to stressful conditions including starvation.
Simple synthesis and functionalisation of α-hydroxyglycine-containing peptide fragments biorxiv.org 2026-06-02 We report here a method for the chemical synthesis of Fmoc-protected -hydroxyglyine (-OH-Gly) dipeptides.
Equitable Health Intelligence: An Open Benchmark of Multi-Population Machine Learning for Omics-Based Cancer Prognosis biorxiv.org 2026-06-02 Purpose: Machine learning (ML) models for omics-based cancer prognosis are often trained on data from predominantly European-ancestry populations, pro
Genome Organizer SATB1 selectively activates a defined subset of EMT genes driving metastatic breast cancer biorxiv.org 2026-06-02 SATB1 reshapes chromatin architecture and transcriptional programs to promote breast cancer metastasis.
Iron-deficiency in the tumor microenvironment reprograms tumor-immune interactions in a sex biased manner biorxiv.org 2026-06-02 Background: Glioblastoma (GBM) is a sex-biased disease characterized by higher incidence and poorer survival in males.
Pathology-Targeted EP4 Agonism Reverses Fibrosis in a Rat Model of DMD biorxiv.org 2026-06-02 Duchenne muscular dystrophy (DMD) presents a critical therapeutic gap in adolescent patients, where fibro-fatty muscle replacement and depletion of th
Two Genomes, One Metabolome: Mitonuclear Incompatibility Remodels Developmental Metabolism and Fitness in Drosophila biorxiv.org 2026-06-02 Mitochondrial-nuclear (hereafter mitonuclear) genetic variation can alter cellular bioenergetics and metabolism via jointly encoding the subunits of o
Harmala alkaloids regulate cell division planes in plants biorxiv.org 2026-06-02 Plants produce a vast diversity of specialized metabolites that function as chemical defenses against herbivores, pathogens, and competing plants.
Rank dependency of rescaled pruning in recurrent neural networks biorxiv.org 2026-06-02 Throughout development and maturity, neural circuits undergo massive synaptic pruning, yielding highly sparse connectivity while preserving robust pop
A genetic tool targeting brain GPR75 biorxiv.org 2026-06-02 G-protein-coupled receptor 75 (GPR75) has emerged as an important mediator in diet-induced obesity (DIO) and a promising therapeutic target for anti-o
DeMoDa: A global open access database for comparative research in human dental morphological variation biorxiv.org 2026-06-02 Human dental morphology is diverse and varies within and between populations.
Fine-scale landscape genomics show asymmetric patterns of gene flow for the invasive mosquito Aedes albopictus biorxiv.org 2026-06-02 Mosquito-borne viruses like dengue, Zika, and chikungunya pose increasing health risks in the United States due to the expanding range of Aedes albopi
Gigabase-scale deletion scanning of the human genome biorxiv.org 2026-06-02 What fraction of the human genome is essential for cellular viability?
Alginate-Coated Collagen Hydrogel Tubes for Scalable Cell and Biotherapeutic Particle Manufacturing biorxiv.org 2026-06-02 Efficient, scalable, and cost-effective production of mammalian cells and biotherapeutic particles remains a major challenge for both research and cli
A Pan-Cancer Multi-Omic SuperLearner for Regulated Cell Death Survival Topologies biorxiv.org 2026-06-02 Introduction: Regulated cell death (RCD) pathways profoundly influence tumor progression and immune modulation.
Elasto-Osmotic Phase Separation in Confluent Cellular Tissues biorxiv.org 2026-06-02 Biomolecular condensates that form via liquid-liquid phase separation (LLPS) of, most prominently, intrinsically disordered proteins (IDPs) are ubiqui
A functional amyloid scaffold shapes insect egg coats biorxiv.org 2026-06-02 Functional amyloids serve as structural scaffolds across biology, yet the molecular architecture and assembly principles of many remain unresolved.
Proton FLASH preserves neurocognition across delivery techniques: implications for clinical translation in pediatric brain tumors biorxiv.org 2026-06-02 Background: Radiation therapy is integral to the curative treatment of childhood brain tumors but contributes to late neurocognitive impairment in sur
Circular RNA aptamers act as superb sub-stoichiometric chaperones to prevent cytosolic TDP-43 condensation biorxiv.org 2026-06-02 Pathological protein misfolding and aggregation underlie many devastating human diseases, yet strategies to selectively neutralize aggregation-prone p
The Transcription Factor Sob Maintains Intestinal Stem Cell Homeostasis to Delay Ageing biorxiv.org 2026-06-02 Ageing is associated with physiological decline and disrupted tissue homeostasis, often driven by imbalanced stem cell activity.
POCKET-seq enables genome-wide profiling of on- and off-target transcriptional regulation events by dCas9-KRAB during CRISPR interference experiments biorxiv.org 2026-06-02 CRISPR interference screens use catalytically inactive dCas9 fused to a repressor domain to enable genetic perturbations at the transcriptomic level.
Small Molecule-Directed RNA Modification via Proximity-Driven Catalysis biorxiv.org 2026-06-02 Selective chemical modification of RNA is essential for RNA functionalization, probing RNA structure-function relationships and developing RNA-targete
Extrahepatic, cell-specific delivery of LNPs through competitive inhibition of ApoE-mediated uptake biorxiv.org 2026-06-02 Targeted lipid nanoparticles (LNPs) for extrahepatic drug delivery are limited by apolipoprotein E (ApoE)-mediated hepatic accumulation.
Extracellular chaperones modulate AL light chains fibrillar aggregation and contribute to amyloid structural heterogeneity biorxiv.org 2026-06-02 In AL amyloidosis, monoclonal immunoglobulin light chains (LCs) aggregate as amyloid fibrils in tissues.
ReCardioids: a robust human heart organoid platform to model doxorubicin- and radiotherapy-induced cardiotoxicity biorxiv.org 2026-06-02 As global cancer incidence rises, so does the population of cancer survivors, making long-term and post-treatment quality of life a central clinical c
Toward Large-Scale Preclinical Neuroimaging: Quantifying Inter-Scanner Variability in Mouse Brain MRI biorxiv.org 2026-06-02 Multi-site MRI studies in preclinical neuroimaging are emerging, but unlike in human studies, characterization of inter-scanner variability remains li
Heterologous iron-sulfur cluster biogenesis and delivery for cytosolic isobutanol and isopentanol production in Saccharomyces cerevisiae biorxiv.org 2026-06-02 Saccharomyces cerevisiae is an excellent microbial platform for sustainable production of next generation biofuels such as the branched chain higher a
Capturing Regional Variation in Aortic Mechanics: Dual-Estimation Method for Material Parameter Identification and Biological Correlation biorxiv.org 2026-06-02 The aorta shows significant regional variation in geometry and composition.
Mechanistic Interpretability for Protein Language Models: A Validation Framework biorxiv.org 2026-06-02 Protein language models (PLMs) are shown to be powerful predictors of protein structure and function but their internal mechanisms remain poorly under
Decoding the Grammar of Protein-Protein Interaction Interfaces with Multimodal Representations biorxiv.org 2026-06-02 Protein-protein interactions (PPI) govern essential cellular processes, making the computational identification of interacting sites a central challen
ATI_Box: A Simple tool for convolutional neural network-based image semantic segmentation biorxiv.org 2026-06-02 Quantitative analysis of microscopic images has become a standard in basic biological and biomedical research.
PepForge: Hierarchical HELM-Based Peptide Generation biorxiv.org 2026-06-02 Peptides carrying special connections such as macrocyclizations and various other structural modifications constitute a major class among peptide ther
Spatial transcriptomic analysis reveals coordinated gene expression in ovarian clear cell carcinoma and adjacent endometriosis in UK and Japanese patients biorxiv.org 2026-06-02 Purpose: Ovarian clear cell carcinoma (OCCC) is strongly associated with endometriosis and shows geographic variation in incidence.
Propagation mode shapes contrasting growth strategies through aquaporin networks in onion biorxiv.org 2026-06-02 Propagation mode strongly influences crop establishment, yet its impact on whole-plant transport strategies remains poorly understood.
A neural circuit mechanism for abstract free choice biorxiv.org 2026-06-02 Making abstract free choices, such as arbitrary decisions between comparable options formed independently of specific motor plans, is central to every
Category selectivity observed in the human brain is distinct from category selectivity observed in artificial neural networks biorxiv.org 2026-06-02 Category selectivity for images of faces, scenes, and bodies is among the most striking and reproducible findings in vision neuroscience.
MRI-Compatible Rigid Head Holders for Artifact-Free Multimodal Imaging in Mice biorxiv.org 2026-06-02 Purpose: High-resolution intravital microscopy allows cellular-scale analysis of the brain in vivo but is greatly sensitive to physiological motion.
Androgen depletion increases sensitivity to effort-related costs and alters mesoaccumbal circuit function in male mice biorxiv.org 2026-06-02 Background: Androgen deficiency in males is associated with reduced motivation, fatigue, and decreased goal-directed behavior, yet the neural mechanis
Developmental Chlorpyrifos Exposure Disrupts Tectal Circuit Formation and Behavior in Xenopus laevis biorxiv.org 2026-06-02 Developmental exposure to organophosphate pesticides has been associated with adverse neurodevelopmental outcomes, but the circuit-level mechanisms un
Impaired Sensory-motor Reconfiguration and Pupil-linked Arousal in Aging biorxiv.org 2026-06-02 Mounting evidence indicates impairments of decision-making and brainstem arousal systems in aging.
Neural graft elimination via dual safety switch compromises therapeutic recovery after stroke biorxiv.org 2026-06-02 Safety switch systems are increasingly incorporated into stem cell therapies to enable selective graft elimination in the event of adverse outcomes.
Degraded myelin is associated with cold hypersensitivity in paclitaxel-induced peripheral neuropathy biorxiv.org 2026-06-02 Cold allodynia is a debilitating symptom of chemotherapy-induced peripheral neuropathy (CIPN), a side effect of many chemotherapeutics, including pacl
Voxel-wise tracer kinetic model selection for DCE-MRI measurements of blood-brain barrier leakage biorxiv.org 2026-06-02 Purpose: To apply voxel-wise tracer kinetic model selection, characterise the spatial distribution of best-fitting models across the brain, and evalua
HEALTHY AGING AS INFORMATION DIVERGENCE IN THE MULTIPLEX BRAIN biorxiv.org 2026-06-02 Understanding the co evolution of the human brain s structural scaffold and functional traffic across the adult lifespan remains a fundamental challen
Enabling Stable Cortical Reconstruction in the HCP Pipeline at Standard Resolution Using FastSurfer Integration with Hybrid T2w- and T1w-based Masking biorxiv.org 2026-06-02 This study aimed to enable stable cortical reconstruction within the Human Connectome Project (HCP) pipeline under standard-resolution (1 mm3) conditi