NMDA Receptor Subunit NMR-2 Regulates Pathogen-Induced Immune Responses via the Nervous System in C. elegans biorxiv.org 2026-06-02 Neural control of innate immunity must balance restraint of basal immune activity with rapid activation upon pathogen encounter.
Immune activation during broadly neutralizing antibody-mediated HIV suppression prior to post-intervention control biorxiv.org 2026-06-02 Broadly neutralizing antibodies (bNAbs) have been associated with enhancement of HIV-specific T or B cell responses and sustained partial control of H
immgenT CD4: a reference landscape of mouse CD4+ T cell biorxiv.org 2026-06-02 CD4+ T cells are orchestrators of the immune system with diverse effector functions.
Altering the ribosome exit tunnel to improve consecutive incorporation of challenging monomers biorxiv.org 2026-06-02 Ribosomes are capable of incorporating a wide array of natural and unnatural monomers into growing polymer chains, but can be stalled by monomers with
Quantitative biophysical analysis of human septin hexamer and octamer self-assembly on model membranes biorxiv.org 2026-06-02 Septins are GTP-binding cytoskeletal proteins that shape and compartmentalize the plasma membrane.
Simulation-driven discovery of morphology-function relationships in microswimmers biorxiv.org 2026-06-02 For more than a billion years, microorganisms have evolved complex strategies for navigating aquatic habitats, despite the fundamental limitations and
Single-molecule visualisation of human Hsp70-driven conformational remodelling during stress biorxiv.org 2026-06-02 The Hsp70 chaperone system plays a central role in the folding of nascent polypeptides and in preventing protein misfolding and aggregation during cel
In-cell structures visualize human pre-ribosome assembly in the nucleolus biorxiv.org 2026-06-02 Ribosome biogenesis is an essential multi-step process.
CPAP/CENPJ is essential for the stability and function of AAA+ ATPase VPS4B biorxiv.org 2026-06-02 Function of CENPJ/CPAP is essential for centriole duplication and cilia biogenesis.
Vermeer: Autoregressive generative modeling of microscopy predicts protein localization biorxiv.org 2026-06-02 Fluorescent microscopy provides a rich view into how proteins localize within cells, but it remains experimentally infeasible to image human proteins
Protocol for assessing lysosomal ion channel function in mammalian cells using lysosomal patch-clamp technique biorxiv.org 2026-06-02 This protocol describes manual whole-endolysosome patch-clamp recordings from pharmacologically or genetically enlarged endolysosomes in cultured mamm
Prostaglandins regulate the nucleoskeleton during Drosophila border cell migration biorxiv.org 2026-06-02 The nucleoskeleton, which is comprised of Lamin A (stiffer), Lamin B, and Lamin interacting proteins, including Emerin, controls nuclear stiffness.
Maternal Gut Microbiota Regulates Neonatal Retinal Angiogenesis Through Lactational Metabolic Signalling biorxiv.org 2026-06-02 Early life vascular development is a tightly regulated process that influences organ maturation and longterm cardiometabolic and neurovascular health.
Declining autophagy drives age-related axonal decay through microtubule alterations biorxiv.org 2026-06-02 The microtubule cytoskeleton plays a pivotal role in maintaining axonal integrity, and its deterioration has been closely associated with neuronal dec
The way less obvious: PIEZO1 supports apoptotic cell extrusion by optimizing tissue mechanical tension for homeostasis. biorxiv.org 2026-06-02 Apical cell extrusion is a mechanical process that allows epithelia to eliminate apoptotic cells and prevent inflammation.
MARBL: A Live-Cell Method for Profiling Bioenergetic Heterogeneity by Noncanonical Methionine Labeling of the Cell Surface Proteome biorxiv.org 2026-06-02 Heterogeneity is a hallmark of biological systems, where cell-to-cell variability supports adaptation to changing environments, but also enables malad
HKDC1 contributes to aberrant lysosome-mitochondria contact in Niemann-Pick disease type C biorxiv.org 2026-06-02 Niemann-Pick disease type C (NPC) is a neurovisceral lysosomal storage disorder comprising two clinically indistinguishable but genetically distinct s
Synphilin-1 mitigates autophagy dysfunction, modulates ubiquitinated protein aggregation, and promotes cell survival during proteotoxic stress biorxiv.org 2026-06-02 The decline of cellular proteostasis is a hallmark of aging and key contributor to neurodegenerative diseases.
Metabolic Intervention with Dimethyl Malonate Impairs Phagocytic Clearance but Fails to Protect Neurons biorxiv.org 2026-06-02 Secondary degeneration following optic nerve crush (ONC) is driven in part by mitochondrial dysfunction and microglial activation.
A genetic model of copulatory wounding in Drosophila melanogaster biorxiv.org 2026-06-02 In the animal kingdom, copulatory wounds occur due to aggressive behavior or injury from the female or male appendages in contact.
Chaperones shape the conformational landscape of 26S-proteasome-base assembly for allosteric ATPase motor activation biorxiv.org 2026-06-02 Protein homeostasis depends on the 26S proteasome, the most complex ATP-dependent protease in eukaryotic cells.
Divergent Inclusion Body Structures and Stabilities Emerge from Native Monomer Properties biorxiv.org 2026-06-02 Protein aggregation is of broad importance in biotechnology and disease, yet the structural heterogeneity of cellular aggregates has confounded high-r
Functional coupling between the a4 - a5 loop and allosteric site on MKP5 is a critical determinant of catalysis biorxiv.org 2026-06-02 Dual-specificity phosphatases (DUSPs) that inactivate mitogen-activated protein kinases (MAPKs) are called MAPK phosphatases (MKPs).
Multimodal dynamics control activity of a glial glutamate transporter biorxiv.org 2026-06-02 Membrane transporters move polar solutes across lipid bilayers to regulate cellular metabolism, signaling, and drug distribution.
Single-Cell Electrophysiology Reveals Verapamil's Disruption of Bacterial Membrane Energetics biorxiv.org 2026-06-02 Verapamil, a clinically used calcium channel blocker, enhances the activity of several tuberculosis antibiotics, but its mechanism of action and physi
Spin-Dependent Extracellular Respiration biorxiv.org 2026-06-02 Biological energy conversion relies on highly efficient electron transfer.
Extracting anomalous diffusion parameters from multi-state ensembles of short single molecule trajectories. biorxiv.org 2026-06-02 Single-molecule tracking measures the stochastic motion of individual biomolecules in the cellular environment.
Synthetic Fibrous Hydrogels as Minimal Systems to Modulate Cell Migration Modes in 3D biorxiv.org 2026-06-02 Cell migration in three-dimensional (3D) environments is highly plastic and regulated by extracellular matrix (ECM) cues.
Physics-guided design of intrinsically disordered proteins biorxiv.org 2026-06-02 Intrinsically disordered protein regions (IDPs) are found across the tree of life and characterized by the lack of a stable 3D fold, encoding function
Hashi: Bridging Statistical Model Derived 1D Microstate Encodings and Protein 3D Structural Ensembles biorxiv.org 2026-06-02 The functioning of proteins is intimately linked to the conformational states they sample within the native ensemble.
Fingertip real contact area scales quadratically with input voltage in electrostatic actuation biorxiv.org 2026-06-02 Touchscreens have become the dominant interface in consumer electronics, yet interactions with them remain primarily visual.
A self-consistent model for phase separation and active processes in biomolecular condensates biorxiv.org 2026-06-02 Biomolecular condensates are thought to play a pivotal role in cellular organization by regulating biochemical reactants in space and time.
Index-agnostic oblique plane light sheet microscopy of centimetre-scale cleared tissues at subcellular resolution biorxiv.org 2026-06-02 We present cleared-tissue direct-view oblique plane microscopy (CtDvOPM), which enables optically sectioned subcellular resolution imaging of centimet
Morphology-robust quantification of subcellular organization in complex cells biorxiv.org 2026-06-01 Quantitative analysis of subcellular protein organization is often confounded by variation in cell morphology, limiting the identification and interpr
Cell-type Specific Alteration of Dicer1 Accelerates Tumor Progression in Mouse Models of KRAS-driven Lung Adenocarcinoma biorxiv.org 2026-06-01 MicroRNAs (miRNAs) have been widely implicated in cancer initiation and progression, yet examination of the effects of global miRNA disruption on thes
Engineering Biosensors to Enhance Monoterpene Indole Alkaloid Production in Yeast biorxiv.org 2026-06-01 Monoterpene Indole Alkaloids (MIAs) are a diverse family of plant natural products with various medicinal applications.
Transcriptomic and proteomic responses to gas vesicle collapse in native and engineered bacterial systems biorxiv.org 2026-06-01 Gas vesicles (GVs) are air-filled protein nanostructures produced by microbes to regulate buoyancy and have emerged as powerful tools in biomedical im
Extracellular vesicles from Manila clam (Ruditapes philippinarum): tailored isolation from hemolymph and insights into water-derived vesicles biorxiv.org 2026-06-01 Extracellular vesicles (EVs) are evolutionarily conserved mediators of intercellular communication released by cells into biological fluids and the ex
Negative HIV serology in perinatally infected children after early treatment and viral suppression: an exploratory analysis of immune correlates biorxiv.org 2026-06-01 Undetectable HIV-specific antibodies in early-treated children with confirmed infection correlate with low viral reservoir and may identify those elig
mRNA-Encoded TLR5 Agonist as an Immune Adjuvant biorxiv.org 2026-06-01 Activation of innate immunity enhances adaptive immune responses and underlies the mechanism of action of vaccine adjuvants.
Unbiased identification of responding T cell clones from longitudinal repertoire sequencing with CloneSearch biorxiv.org 2026-06-01 T cells activate and expand upon interaction with cognate antigen, derived from pathogens or mutated proteins.
Systematic Comparison of Indicator and Pathogenic Viruses Using High-Throughput qPCR Identifies Pepper Mild Mottle Virus as a Robust Indicator of Virus Removal in Wastewater Treatment biorxiv.org 2026-06-01 The selection of appropriate viral indicators for evaluating wastewater treatment performance remains challenging because candidate markers have rarel
A replication hijacking mechanism for Tn3-family replicative transposition biorxiv.org 2026-06-01 Transposition of all classes of transposable elements generates DNA intermediates that must be processed by the host to be effective.
Hypertension-mediated cardiac fibrosis is a mechanical process initiated by smooth muscle cells biorxiv.org 2026-06-01 Hypertension represents the most prevalent chronic cardiovascular condition, typically culminating in pathological cardiac remodeling characterized by
Immune context unmasks regulatory effects of Neanderthal and Denisovan introgression biorxiv.org 2026-06-01 Neanderthal and Denisovan introgression have left a pervasive footprint in the human genome, yet its regulatory consequences remain poorly understood.
New approaches to detecting and characterizing introgression in large species trees biorxiv.org 2026-06-01 Many current phylogeny-based methods to detect introgression use samples of species-quartets to detect asymmetries in gene tree frequencies.
Efficient base editing and development in human embryos without chromosomal alterations biorxiv.org 2026-06-01 Cas9-based tools enable the introduction of genetic lesions to investigate DNA repair outcomes and edit the genome at disease-relevant loci.
Histone succinylation directly inhibits Jumonji domain demethylases and stabilizes repressive chromatin states biorxiv.org 2026-06-01 Herein we uncover a relationship between histone succinylation and Jumonji (JmjC) domain-containing histone demethylases.
Targeting lncRNA JINR1 with programmable Circular Active Nano DNAzyme (CANDe) suppresses Japanese Encephalitis Virus infection biorxiv.org 2026-06-01 Oligonucleotide therapeutics such as antisense oligonucleotides (ASOs) and small interfering RNAs (siRNAs) enable sequence-specific gene silencing but
Hepatic Acetate is an Essential Fuel for the Heart during Energy-Deprived States biorxiv.org 2026-06-01 Energy supply is fundamental to cardiac performance, yet the mechanisms by which the heart adapts to nutrient scarcity remain incompletely understood.