Tiny Subsamples and Upsampling Tame Big Data Evolutionary Analysis in Phylogenomics biorxiv.org 2026-06-23 Long runtime, high memory demands, and reliance on high-performance computing increasingly limit the evolutionary analysis of long phylogenomic datase
Targeting Dengue Virus NS3 Helicase: Biochemical and Computational Evaluation of Catechins from Camellia sinensis as Potential Therapeutic Leads biorxiv.org 2026-06-23 Dengue Virus Serotype 2 is a human pathogenic flavivirus that encodes a non-structural protein 3 (DEN2-NS3) containing a helicase domain essential for
Fluorescence Blinking Patterns Fingerprint the Local Protein Environment biorxiv.org 2026-06-23 The function of a protein depends not only on its sequence but on post-translational modifications and folding that produce functionally distinct prot
Genomic and Kinetic Modeling Involving Nanoparticle-Mediated Delivery of a Novel Chitinase Enzyme to Outpace Tuta absoluta Damage biorxiv.org 2026-06-23 The tomato leafminer, Tuta absoluta, poses a severe global agricultural threat due to its rapid leaf-mining behavior and swift development of resistan
Phase-tuned interfacial condensates drive transcellular access biorxiv.org 2026-06-23 Life has evolved the plasma membrane as an active physicochemical interface rather than a passive barrier, one whose selectivity sustains homeostasis
AutoBlot: deterministic single-cell western blotting reveals proteomic diversity in rare cell populations biorxiv.org 2026-06-23 Single-cell western blotting (scWB) provides quantitative, molecular-mass-resolved protein measurements but relies on Poisson-limited gravity settling
Elemental Analysis of Herbal Food Supplements using ICP-MS for Toxicant and Nutritional Profiling biorxiv.org 2026-06-23 Botanical dietary supplements (like wheat, barley, teff, oats, white lupin, pumpkin seed, and chickpeas) may contain trace amounts of toxicants in add
Lichen Growth Form Effects on Rock Weathering in Cold Biomes biorxiv.org 2026-06-23 Rock surface weathering is a critical element in the process of early soil formation, in which lichens are thought to play a significant role.
Impact of Amazonian protected areas in preventing deforestation and carbon loss over four decades biorxiv.org 2026-06-23 Native vegetation protection is a key strategy for delivering both biodiversity and climate benefits, and protected areas have been widely adopted to
Mitochondrial Lon protease couples substrate translocation to proteolytic activation biorxiv.org 2026-06-23 Human LonP1 is an ATP-dependent mitochondrial protease that degrades damaged or redundant proteins.
Intrinsic representational drift from predictive excitatory-inhibitory plasticity biorxiv.org 2026-06-23 Neural representations drift gradually over time even under stable environmental conditions, but the synaptic mechanisms driving this drift remain unc
Characterisation of Posterior Predominant Amyloid PET Binding Across Multiple Cohorts biorxiv.org 2026-06-23 The standard approach to quantify amyloid (A{beta}) PET averages uptake within a single cortical mask that assumes no clinically relevant spatial hete
Electrolytic-Microbubble Dynamics Delineate Safety Thresholds During Intracortical Microstimulation with Flexible Neural Interfaces biorxiv.org 2026-06-23 Intracortical microstimulation (ICMS) with ultraflexible neural electrodes enables low-threshold, chronically stable, and high-resolution modulation o
Age Alters the Relationship between Post-Encoding Sleep Quality and Context Memory Neural Reinstatement biorxiv.org 2026-06-23 Sleep supports episodic memory consolidation, yet it remains unclear how naturalistic post-encoding sleep quality relates to the neural reinstatement
Acoustic Characteristics of Low-Frequency Vocalizations of Bryde's Whales (Balaenoptera edeni edeni) in the Weizhou Island Waters of the Beibu Gulf, China biorxiv.org 2026-06-23 Bryde's whales (Balaenoptera edeni edeni) are nationally protected in China, and the waters around Weizhou Island in the Beibu Gulf support one of the
Systematic benchmarking of zero-shot utility and robustness in single-cell transcriptomic foundation models biorxiv.org 2026-06-23 Single-cell foundation models (scFMs) have been proposed as reusable representations for transcriptomic analysis, yet their practical utility and robu
CellOS: Learning a World Model of Cellular State through Joint Embedding Prediction biorxiv.org 2026-06-23 Foundation models learned from single-cell transcriptomes are central to the prospect of AI virtual cell that can represent, query and predict cellula
The Metabolic Scope Theory of Aging: Rising Mitochondrial Impedance Compresses Metabolic Reserve to Constrain Lifespan biorxiv.org 2026-06-23 Maximum lifespan varies more than 100-fold across vertebrates, yet within each species aging emerges as a coordinated syndrome spanning metabolism, im
Transplanted human photoreceptors differentially survive, incorporate, and mature in mildly and severely degenerated mouse retinae biorxiv.org 2026-06-23 Photoreceptor transplantation is considered a disease-agnostic therapeutic strategy for retinal degenerative diseases with highly heterogenous genetic
ABCA7 rs3752231 variant effects on glial responses to amyloid-β and plaque maturation in Alzheimer's disease. biorxiv.org 2026-06-23 Variants in ABCA7 are among the most consistently replicated genetic risk factors for late-onse Alzheimer's disease (AD), yet the cellular mechanisms
Insect-inspired, efficient event-based classification of tactile features biorxiv.org 2026-06-23 Tactile sensing enables humans and animals to detect and discriminate features during exploration and guide context appropriate actions.
Co-existence of Modularity and Anti-modularity in the Functional brain connectomes biorxiv.org 2026-06-23 The brain requires coordination among different regions to execute cognitive tasks, which may involve both positive- and negative-correlations.
PERIODIC AND APERIODIC SPECTRAL SIGNATURES OF BEING MOVED BY ART biorxiv.org 2026-06-23 Intense aesthetic experiences are among the most complex responses arising from the interaction of mind, brain, and context.
Improving the detection sensitivity of calcium transients in densely labeled neuronal tissue with pinhole illumination - A low-cost approach biorxiv.org 2026-06-23 Optical recording from large numbers of neurons is an indispensable technique for studying neuronal ensembles.
Intravenous midazolam alters short-interval paired-pulse TMS responses differently in younger and older adults biorxiv.org 2026-06-23 Objective: Aging is associated with changes in cortical excitability and altered responsiveness to benzodiazepines, but the effects of benzodiazepine
Distinct Endosomal Sorting Complexes Required for Transport Components Differentially Regulate Glutamate and Gamma-Aminobutyric Acid Receptor Surface Expression biorxiv.org 2026-06-22 Endosomal sorting complexes required for transport (ESCRT) regulate membrane protein trafficking through coordinated cargo selection and endosomal pro
Multiscale active transport driven by gravitactic bioconvection promotes resilience in algal blooms biorxiv.org 2026-06-23 Algal blooms are frequently dominated by motile species1,2 whose vertical migration enhances resource acquisition and bloom development3,4.
A mathematical model for the efficient control of the New World screwworm biorxiv.org 2026-06-23 An outbreak of New World screwworm has recently been spreading across Mexico, after more than 30 years of absence.
Acoustic detection of a rarely vocalising invasive mammal from sparse data biorxiv.org 2026-06-23 1. Monitoring and limiting the spread of invasive species on islands requires efficient detection and population estimation methods.
The MosAICo ecosystem: bridging the taxonomic gap in vector surveillance with real-time entomological artificial intelligence biorxiv.org 2026-06-23 Mosquito-borne diseases represent an escalating global health threat, driven by climate change, urbanization, and the spread of invasive vectors into
No silver bullet: Patterns of macrosynteny recapitulate systemic conflicts in the higher-level relationships of the arachnids biorxiv.org 2026-06-23 Rare genomic changes have long been sought by phylogeneticists for their potential to resolve obdurate nodes in the tree of life.
First genetic detection and ongoing eDNA monitoring of the golden mussel (Limnoperna fortunei) in California biorxiv.org 2026-06-23 Here, we report the first genetic confirmation of golden mussels (Limnoperna fortunei) in North America, and the subsequent development, optimization,
Is APOE ε2 always a protective allele? Deviations in Hardy-Weinberg equilibrium in admixed Brazilian elderly individuals biorxiv.org 2026-06-23 The APOE gene is a critical determinant of human healthspan and longevity, with the rare {epsilon}2 allele traditionally viewed as a universal protect
Variation and selection at predicted G-quadruplexes across the human pangenome biorxiv.org 2026-06-23 G-quadruplexes (G4s), non-canonical DNA structures whose sequence motifs occupy approximately 1% of the human genome, are important for myriad cellula
A PLP-Dependent Decarboxylative Mannich Reaction Initiates Construction of the Nonpeptidic Scaffold of Kaitocephalin biorxiv.org 2026-06-23 Kaitocephalin (KCP) is a fungal neuroactive natural product bearing a peptide-like yet nonpeptidic amino acid-derived scaffold in which amino acid-lik
The structure of the lipid II flippase from monoderm bacteria biorxiv.org 2026-06-23 Peptidoglycan biogenesis requires membrane flippases to translocate lipid-linked precursors across the cytoplasmic membrane for processing (1).
Segmental Variability of Bolus-dispersion-induced Myocardial Blood Flow in Quantitative Myocardial Perfusion MRI: A CFD-based Analysis biorxiv.org 2026-06-23 Background: Quantitative dynamic contrast-enhanced myocardial perfusion cardiovascular magnetic resonance (CMR) enables estimation of myocardial blood
Programming T cells for intercellular genome editing biorxiv.org 2026-06-23 Therapeutic genome editing requires delivery of editing molecules to defined cell types, but targeting specificity and efficiency are currently limite
Organoid-in-Bead (OrB): vortex-based compartmentalization enables scalable, high-density intestinal organoid culture biorxiv.org 2026-06-23 Intestinal organoids are powerful in vitro models, but their use in large-scale analyses remains constrained by the low throughput, labor-intensive ha
Particle-Only gECM Wafers Enable Cohesive, ECM-Rich Scaffolds Without Secondary Polymers biorxiv.org 2026-06-23 Granular extracellular matrix (gECM)-based biomaterials commonly contain polymer components to improve scaffold cohesion and handling during fabricati
Cervical spinal cord stimulation disrupts proprioception yet improves voluntary arm reaching biorxiv.org 2026-06-23 Whether proprioception is necessary for upper-limb motor control has been debated for decades.
biomeStat: Using Agentic AI for Scalable Genomic Epidemiology Demonstrated Through End-to-End Analysis of 1,000 Asian Dengue Virus Genomes biorxiv.org 2026-06-23 Genomic epidemiology workflows typically require expert curation of multiple specialized tools, extensive manual parameter tuning, and access to heter
The avian colourscape is disproportionately threatened by species extinctions biorxiv.org 2026-06-23 The impact of projected extinctions on global animal colour diversity remains unknown.
The Dark Ecology Dataset: Measurements of Aerial Biomass in US Weather Radar from 1995 to 2025 biorxiv.org 2026-06-23 The US NEXRAD radar network has monitored the aerosphere over the US and its territories continuously since the 1990s and archived nearly 300 million
Testing the waters of macrophyte biodiversity with multiscale spatial analysis of public lake monitoring data biorxiv.org 2026-06-23 Freshwater monitoring programmes like the European Union's Water Framework Directive (WFD) provide a wealth of data on European lake status, including
TickMapKB: A FAIR Spatial Knowledgebase of Tick Species and Associated Pathogens in India biorxiv.org 2026-06-23 Ticks transmit pathogens across wildlife, domestic animals, and humans, and are therefore considered important vectors under the One Health framework.
Lessening the bottleneck: reduced spatiotemporal overlap between krill fishing vessels and post-fledging chinstrap penguins led to increased apparent survival biorxiv.org 2026-06-23 Fledging is a critical period of a seabird life cycle.
A maximum entropy perspective reveals deviations from steady state during active diversification biorxiv.org 2026-06-23 Ecosystems are rarely at steady state, yet most theory predicting universal biodiversity patterns assumes they are.
Smolt migration in relation to latitude, season and migratory distance biorxiv.org 2026-06-23 Downstream migration and sea entry are periods of high mortality for sea trout smolts and migration timing is a critical aspect of survival.
Ancient origins of alkaloid biosynthesis in medicinal clubmosses biorxiv.org 2026-06-23 The plant kingdom is rich with medicinal natural products that are complex and difficult to access.