A Visually Interpretable Histopathology-Based Immune Model Predicts T-effector Biology and Response to Immune checkpoint inhibition in Clear Cell Renal Cell Carcinoma Clinical Trial and Contemporary Real-World Datasets biorxiv.org 2026-06-25 Immune checkpoint inhibitors (ICI) are central to the treatment of metastatic clear cell renal cell carcinoma (ccRCC), yet only a subset of patients d
Ambiguity-Aware Multi-Stage Cell-Type Annotation for Spatial Transcriptomics biorxiv.org 2026-06-25 Spatial transcriptomics enables characterization of cellular organization in intact tissue, but robust cell type annotation remains challenging due to
Evidence for post-allopolyploidy genetic exchanges between duplicated regions in three ancient polyploidies biorxiv.org 2026-06-25 Many successful lineages, including flowering plants and vertebrates, owe some of their evolutionary prosperity to whole genome duplications (WGD).
GenoSim: A Forward-Time Genotype Simulator for Clinical and Population Genetics with Population Stratification biorxiv.org 2026-06-25 Motivation: Next-generation sequencing studies in clinical genetics are often limited by the scarcity of human genotype data, which stems from ethical
Interplay Between Protein-RNA Binding and Phase Separation Drives Emergent Behavior in RNP Condensates biorxiv.org 2026-06-25 Protein-RNA binding and biomolecular condensation are two key processes underlying the assembly and function of ribonucleoprotein (RNP) condensates.
The Staphylococcus aureus carotenoid staphyloxanthin modifies the structure of phosphoglycerol lipid bilayers biorxiv.org 2026-06-25 Staphyloxanthin (STX) is a carotenoid synthesized by the human pathogen Staphylococcus aureus.
Scalable Plasmonic Metasurface-Enabled Physics-Guided Self-Supervised Cellular Imaging biorxiv.org 2026-06-25 High-quality cellular imaging, especially in live cells, remains constrained by the trade-off among signal-to-noise ratio, phototoxicity, and instrume
F.A.D.E. (Fully Agentic Drug Engine): A Conversational AI Platform for Drug Discovery biorxiv.org 2026-06-25 Drug discovery remains one of the costliest and most time-intensive endeavors in the pharmaceutical pipeline, with average development costs exceeding
A Zebrafish Platform to Model Human SCN2A and SCN8A Epilepsy and Evaluate Anti-Seizure Medications biorxiv.org 2026-06-25 Many patients with epilepsy have inadequate seizure control using current anti-seizure medications (ASMs), illustrating the need for new treatments.
Dynamic resource allocation orchestrates physical simulation in the human brain biorxiv.org 2026-06-25 Humans can flexibly predict physical events by internally simulating object dynamics in the brain, a capacity lacking in current AI systems.
A humanized Galleria mellonella model reveals prophage-mediated breakdown of colonization resistance against Salmonella biorxiv.org 2026-06-25 Background Colonisation resistance provided by the gut microbiota is a critical barrier to pathogen invasion, yet its study in vivo is constrained by
Intracellular amino acid scarcity sensing tunes protein hunger biorxiv.org 2026-06-25 Protein is a key macronutrient that strongly governs food intake.
NeuroEnergetics-on-Chip: a novel 3-compartment microfluidic platform to study metabolic interactions between brain parenchyma and cerebral vasculature biorxiv.org 2026-06-25 Neurological disorders are a major cause of death and disability worldwide.
Decomposition of task-specific responses in the multiple demand network biorxiv.org 2026-06-25 Neuroimaging evidence suggests that a distributed network of brain areas, known as the multiple demand (MD) network, is consistently recruited across
Dopamine projections to the basolateral amygdala enable reward prediction biorxiv.org 2026-06-25 Reward predictions are critical to both adaptive learning and decision making.
A p53-ΔNp73 signaling axis drives selective motor neuron degeneration in spinal muscular atrophy biorxiv.org 2026-06-25 Selective neuronal vulnerability is a hallmark of many neurodegenerative diseases, yet how ubiquitous genetic insults cause highly selective neuronal
ST3GAL3 loss-of-function disrupts synaptic integrity and excitatory/inhibitory cortical dynamics biorxiv.org 2026-06-25 This study examined the role of ST3GAL3 as a regulator of excitatory/inhibitory (E/I) synaptic homeostasis using a human iPSC-based model.
Stimulus and circuit contributions to the information geometry of neural manifolds biorxiv.org 2026-06-25 Understanding how network connectivity shapes neural representations is central to systems neuroscience.
Neurodevelopment Trajectory of Electrophysiological Functional Connectivity Following Alcohol Use Initiation biorxiv.org 2026-06-25 Background: Adolescence is characterized by profound neurodevelopmental changes that shape large-scale brain network organization and may confer vulne
JNJ-42153605, a mGluR2 PAM, potentiates Levetiracetam treatments of TBI to mitigate subsequent tau aggregation in a larval zebrafish model biorxiv.org 2026-06-25 Traumatic brain injury (TBI) has long-term consequences that include chronic traumatic encephalopathy (CTE) and an elevated risk for Alzheimer Disease
Exploring the functionality of market-available tools for neural recording biorxiv.org 2026-06-25 Low-cost and open-source neural recording systems are increasingly important for expanding access to electrophysiological research.
Dysfunction of the colliculus-pulvinar pathway in children with developmental dyslexia biorxiv.org 2026-06-25 While evidence suggests magnocellular deficits in the geniculostriate pathway in adults with dyslexia, neural deficits in the subcortical pathways dur
Methamphetamine-induced disruption of neuropeptide expression in mice biorxiv.org 2026-06-25 Psychoactive and psychotoxic drugs are particularly harmful, if their use coincides with critical developmental windows of brain maturation.
Sexually dimorphic behavioural signatures of tau toxicity in adult Drosophila biorxiv.org 2026-06-25 Tau pathology is central to Alzheimers disease and related tauopathies, yet mechanisms driving neuronal dysfunction and degeneration downstream of pat
Btbd11 regulates glutamatergic synapse organization in GABAergic inhibitory interneurons biorxiv.org 2026-06-25 The mechanisms that underlie glutamatergic synapse organization and function in GABAergic inhibitory interneurons (INs) are not well described, despit
Medullary epithelium-free areas in the rat thymus are specialized niches enriched for mature thymocytes and distinct stromal subsets biorxiv.org 2026-06-25 The thymic medulla provides the microenvironment for negative selection, late thymocyte maturation, and thymocyte egress, and is generally characteriz
Specific killing of Ewing sarcoma by TCR-T cells targeting public neogene-encoded antigens biorxiv.org 2026-06-25 EWSR1::FLI1, the oncogenic chimeric transcription factor driving Ewing sarcoma (EwS) induces expression of exquisitely EwS-specific neogenes (Ew_NGs)
Continuous mark-release recapture to improve estimates of movement and survival of the African malaria mosquitoes biorxiv.org 2026-06-25 Despite extensive efforts to understand the population biology and ecology of the African malaria mosquitoes questions regarding their movement patter
Coral settlement module designs for scalable reef restoration biorxiv.org 2026-06-25 The global coral reef crisis has prompted restoration initiatives worldwide.
Emerging Beetle-Pathogen Symbioses and Their Consequences for Forest Health: Lessons from Rapid 'Ohi'a Death in Hawai'i biorxiv.org 2026-06-25 Invasive ambrosia beetles and fungal pathogens threaten forest ecosystems worldwide, exemplified in Hawai'i by the widespread loss of keystone species
PARP1 Exhibits an Enzymatically Inactive Chromatin Binding Mode biorxiv.org 2026-06-25 Poly (ADP-ribose) Polymerase 1 (PARP1) is an abundant nuclear enzyme that dynamically engages chromatin in diverse cellular scenarios.
Genetic Code Expansion, Enzymatic Modification, and C-Terminal Labeling Enable Facile Production of Highly Modified α-Synuclein biorxiv.org 2026-06-25 -Synuclein (S), a protein that plays a central role in Parkinson's disease and related synucleinopathies, is an intrinsically disordered protein (IDP)
Self-assembling nanoparticles to assess multivalent interactions between influenza A virus hemagglutinin and glycan surfaces biorxiv.org 2026-06-25 The multivalent display of surface glycoprotein hemagglutinin (HA) on Influenza A viruses (IAVs) enhances the overall binding avidity to sialylated gl
Tracing the Path from 4-Hydroxyphenylpyruvate to the Benzoquinone Ring of Q6 and the p-aminobenzoate pathway in Yeast biorxiv.org 2026-06-25 Coenzyme Q (ubiquinone, CoQ) is an essential component of the mitochondrial electron transport chain and a major lipid antioxidant in eukaryotic cells
High-Throughput Screening Identifies Small-Molecule Inhibitors of the Tau-LRP1 Interaction biorxiv.org 2026-06-25 The cellular uptake and propagation of tau are central features of tauopathies, including Alzheimers disease, and are mediated by the endocytic recept
Confocal Raman Microscopy-Guided Optimization of Early Otic Differentiation from Human Pluripotent Stem Cells biorxiv.org 2026-06-25 Generation of otic progenitors from pluripotent stem cells requires precise timed regulation of signalling pathways, including bone morphogenetic prot
Lipid Network Crosslinked Hydrogels: Controlling MaterialDynamics Across Multiple Length Scales Through Lipid Movement biorxiv.org 2026-06-25 Control over network dynamics at different length scales is a feature of natural materials challenging to replicate in synthetic hydrogels.
Mismatch repair dissection by in vivo RNAi reveals dose-dependent modulators of somatic instability and proteome remodeling in Huntingtons disease biorxiv.org 2026-06-25 Human and mouse genetics have established mismatch repair (MMR) as a central mediator of somatic repeat expansion, a key pathogenic process in Hunting
Antifungal resistance mechanisms and nosocomial transmission of Nakaseomyces glabratus: genomic investigation and observational study in Melbourne, Australia biorxiv.org 2026-06-25 Nakaseomyces glabratus (Candida glabrata) is a WHO high-priority fungal pathogen associated with fungal antimicrobial resistance (fAMR).
DextraDemixer enables accurate identification of antigen-specific T cells from pMHC multimer experiments biorxiv.org 2026-06-25 Antigen specificity of T cells defines the adaptive immune response, yet the vast majority of known T cell receptors (TCRs) lack annotated antigen tar
Do nanoplastics reshape microglial support of neuronal resilience? A study of microglial bioenergetics and microglia to neuron communication in vitro biorxiv.org 2026-06-25 Nanoplastics (NPs) are emerging environmental contaminants able to cross biological barriers, disrupt cellular and organelle homeostasis, and alter th
Identifying Novel Estrogenic Mitochondrial Targets in Hypothalamic Proopiomelanocortin Neurons by Chemoproteomics biorxiv.org 2026-06-25 Loss of estrogens at menopause is linked to impaired brain metabolism and increased risk of Alzheimers disease (AD).
Macroevolutionary consequences of twin neck innovations in deep-sea dragonfishes biorxiv.org 2026-06-25 The origin of novel phenotypes can influence access to new ecological resources, which may have positive, neutral, or negative effects on subsequent p
The crabeater seal reference genome reveals hallmarks of persistently large effective population size and sustained population expansion in the World's most abundant pinniped biorxiv.org 2026-06-25 Population genetic theory predicts that a species demographic history shapes patterns of genome-wide variation.
Tachykinin neuropeptides are involved in axonal and synaptic differentiation of the pioneer motor axon in zebrafish biorxiv.org 2026-06-25 In contrast to many other developing systems, in which axon pathfinding and synaptogenesis are separated in time, the pioneering axon of the individua
An extraglomerular relay circuit for multimodal integration within the Drosophila antennal lobe biorxiv.org 2026-06-25 The central integration of sensory information within and between brain hemispheres is critical for efficient behavioral responses, but at which level
Expanded protocadherin-1 usage reveals a broader hantavirus entry landscape biorxiv.org 2026-06-24 Mammalian hantaviruses are RNA viruses that cause hantavirus cardiopulmonary syndrome in the Americas and hemorrhagic fever with renal syndrome in Eur
Three Plasmid Strategies, One Intermediate Convergence State: Lineage-Specific Resistance, Virulence Architecture in Dominant Indian Carbapenem-Resistant Klebsiella pneumoniae Clones biorxiv.org 2026-06-24 Abstract Carbapenem-resistant Klebsiella pneumoniae (CRKp) is a critical global healthcare threat driven by high-risk multidrug-resistant (MDR) clones
Assessing Corynebacterium glutamicum as a surrogate of Mycobacterium tuberculosis for DNA gyrase inhibitor design. biorxiv.org 2026-06-24 DNA gyrase is an essential bacterial enzyme and a clinically validated target for the treatment of tuberculosis.
Comprehensive Profiling of Monkeypox Virus Antigens Identifies Potent Targets for Next-Generation mRNA Vaccine Development biorxiv.org 2026-06-24 The 2022 Monkeypox virus (MPXV) outbreak renewed interest in vaccines for orthopoxviruses.