Identification of the Down syndrome critical region 3 gene as a mammalian cell size regulator biorxiv.org 2026-07-24 Using a genetic screening approach based on an inducible gene-activating system and cell sorting, Down syndrome critical region 3 (DSCR3) was isolated
Spatial control of mitochondrial retrograde signaling by nuclear pore-associated contact sites biorxiv.org 2026-07-24 Mitochondria relay their functional state to the nucleus via retrograde signaling, yet whether the spatial organization of the mitochondrial network p
Impaired synthesis of both telomere strands and adaptive TERT reduction in RTEL1 ATPase-dead cells biorxiv.org 2026-07-24 Regulator of Telomere Elongation Helicase 1 (RTEL1) plays a critical role in telomere replication by disassembling DNA secondary structures such as G-
Self-supervised representations reveal the genetic architecture of human cortical folding biorxiv.org 2026-07-24 Cortical folding emerges during fetal development, is under genetic control, and remains stable throughout life, offering a lasting window into early
An Integrated Multi-omics Single Cell Atlas of the Human RPE and Choroid biorxiv.org 2026-07-24 The retinal pigment epithelium and choroid are critical for supporting the function and maintaining the homeostasis of the outer retina, and their dys
G-quadruplex folding uncouples cis-activation from collateral trans-cleavage by Cas12a biorxiv.org 2026-07-24 The CRISPR/Cas12a system is widely used in nucleic-acid diagnostics because Cas12a couples guide-directed recognition of a cis-target with indiscrimin
Machine Learning-Assisted Evolution of Broadly Functional Enzyme Libraries biorxiv.org 2026-07-24 Biocatalysis offers sustainable solutions to pressing challenges in chemical synthesis by exploiting the remarkable efficiency and selectivity of enzy
Intratumoral dose heterogeneity promotes adaptive anti-tumor immunity and predicts clinical response to radiopharmaceutical therapy biorxiv.org 2026-07-24 Radiopharmaceutical therapies (RPT) deliver non-uniform radiation dose in tumors and the impact of this on response is poorly understood.
Metabolic engineering of Escherichia coli to modulate hydrogen sulfide levels in the mammalian gut biorxiv.org 2026-07-24 Hydrogen sulfide (H2S) is a microbiota-derived metabolite in the gastrointestinal tract implicated in a number of diseases.
Network-scale disruption of RNA-protein interactions by a bioaccumulating small-molecule drug biorxiv.org 2026-07-24 Many small-molecule drugs accumulate in both host and gut microbial cells.
Network topology determines neuronal phase-of-firing codes biorxiv.org 2026-07-24 Brain rhythms organize neural activity in time.
Microbial Immunomodulatory Function is Altered in Inflammatory Bowel Disease Independent of Microbial Composition biorxiv.org 2026-07-24 Inflammatory bowel diseases (IBD) are chronic and relapsing immune-mediated conditions in which the NF-{kappa}B and STAT3 signalling pathways play cen
Chromosome-scale CRISPR screening reveals secretory pathway genes as drivers of aneuploidy-mediated antifungal tolerance biorxiv.org 2026-07-24 The gain or loss of chromosomes in eukaryotes often drives aberrant phenotypes by altering the expression levels of hundreds or thousands of genes.
Intersection of genotype and environment on the dynamics of the virome and microbiome of an estuarine cnidarian biorxiv.org 2026-07-24 Estuaries host a rich diversity of microorganisms, creating opportunities for animal-microbe interactions that shape host physiology.
How are evolutionarily young and old proteins distributed in sequence space? biorxiv.org 2026-07-24 Protein sequence space is vast due to the combinatorial diversity of 20 amino acids.
Mitochondrial introgression in North American red-backed voles is facilitated by co-introgression at nuclear-encoded mitochondrial genes biorxiv.org 2026-07-24 Mitochondrial genomes encode for the proteins and RNAs that serve as the basis for aerobic respiration and energy production.
Recombination on 'non-recombining' sex chromosomes: empirical insights from the blackspotted stickleback biorxiv.org 2026-07-24 One hallmark of sex chromosome evolution is recombination suppression driven by inversions.
Proteogenomic analysis of the differential stability of cardiac protein isoforms biorxiv.org 2026-07-24 Alternative splicing is an important regulatory layer in gene expression, but knowledge on the isoform protein molecules continue to lag their canonic
A Microgel-Based Platform for Tunable Expansion and Function of γδ T-cells biorxiv.org 2026-07-24 Current {gamma}{delta} T-cell expansion protocols often sacrifice functionality for yield and largely ignore the context of activation.
Epigenetic Regulation of Stable SARS-CoV-2 RBD-sfGFP Expression in Primary Human Splenic Fibroblasts biorxiv.org 2026-07-23 Background: Recombinant expression of the SARS-CoV-2 receptor-binding domain (RBD) is essential for vaccine development, serological diagnostics, and
The Metabarcoding Analysis Pipeline (MAP): Simple, accurate, and flexible metabarcoding biorxiv.org 2026-07-23 Current metabarcoding pipelines are inflexible with respect to study design and are poorly suited to long-read sequence data.
SARS-CoV-2 nucleocapsid protein engages with viral RNA and ERGIC lipids to drive viral core assembly biorxiv.org 2026-07-23 Severe acute respiratory syndrome coronavirus 2 assembles at the ER Golgi intermediate compartment (ERGIC), yet the molecular basis of nucleocapsid (N
Microbial odours activate protective immune response in Caenorhabditis elegans via specific olfactory neurons biorxiv.org 2026-07-23 Caenorhabditis elegans, like other animals, relies on attractive odours for foraging and on aversive odours for avoidance of pathogens.
Plasmodium blood-stage induces trained immunity in hepatocytes biorxiv.org 2026-07-23 Plasmodium parasites, the causative agents of malaria, are transmitted at high levels in endemic areas and sequential infections are common.
RBM6-mediated alternative splicing facilitates the adaptation of Eurasian avian-like H1N1 swine influenza virus biorxiv.org 2026-07-23 The Eurasian avian-like (EA) H1N1 swine influenza virus (SIV), derived from avian influenza viruses (AIV), poses a serious threat to public health due
A genetic screen unveils the polymerization cycle of the bacterial actin-like MreB biorxiv.org 2026-07-23 MreB, a bacterial actin homologue that organizes cell wall synthesis in most rod-shaped bacteria, assembles into membrane-associated filaments through
Genetic Diversity and Antifungal Susceptibility among Ecological Niche Populations of Aspergillus flavus in Yaounde, Cameroon biorxiv.org 2026-07-23 Aspergillus flavus is a ubiquitous fungus commonly found in a variety of ecological niches including soil, crops, the air, and humans.
Human rhinovirus 16 impairs macrophage cytokine secretion by disrupting NF-κB nuclear translocation and intracellular cytokine trafficking biorxiv.org 2026-07-23 Human rhinovirus (HRV) infections are a major cause of acute exacerbations in chronic obstructive pulmonary disease (COPD), often promoting secondary
Single anticodon-edited tRNA therapy targeting highly prevalent Arg>Ter premature termination codons causing inherited retinal diseases biorxiv.org 2026-07-23 Nonsense variants cause 18% of inherited retinal diseases (IRDs), yet current therapies require variant-specific development, leaving most patients un
Widely used GWAS methods can be poorly suited to SNP-level localization under diffuse polygenic architecture in livestock biorxiv.org 2026-07-23 In livestock populations, genome-wide association studies (GWAS) can produce strong, apparently localized associations even when no truly discrete nea
Integrating epigenomic features reveals principles of chromatin-state organization biorxiv.org 2026-07-23 The coordinated activities of histone modifications and chromatin-associated proteins establish chromatin states that regulate genome function and cel
Perturb-seq resolves physiologic programs and bidirectional regulation of non-canonical NF-κB signaling in epidermal organoids biorxiv.org 2026-07-23 Regulated keratinocyte differentiation is required for formation of the stratified epidermis and a functional barrier.
A germline shortcut in protein language model retrieval of adaptive immune receptors biorxiv.org 2026-07-23 Protein language models (PLMs) are increasingly used to represent adaptive immune receptors; however, their advantages over classical alignment-based
Conformational dynamics of exopolysaccharides underlie biofilm matrix mechanics in Vibrio cholerae biorxiv.org 2026-07-23 Polysaccharides remain the least understood biomacromolecules, particularly in terms of the relationship between their chemical structure and physical
Noncanonical Circular RNAs and Potential Functions biorxiv.org 2026-07-23 Circular RNAs (circRNAs) are ubiquitous in eukaryotes; dysregulated circRNA expression is linked to diseases, including lung cancer.
An improved Stentor coeruleus genome and time-resolved transcriptomics link cyclic nucleotide-dependent kinase signaling to single-cell habituation biorxiv.org 2026-07-23 The giant ciliate Stentor coeruleus is a single cell capable of modifying its behavior through experience.
Miniaturized Lysosome Enrichment through Selective Plasma Membrane Destabilization biorxiv.org 2026-07-23 Organelle enrichment presents a prerequisite for the unbiased biochemical analysis of cellular subcellular compartments.
The Holliday junction resolvase GEN1 preserves genome integrity and self-renewal in mouse embryonic stem cells biorxiv.org 2026-07-23 The maintenance of pluripotent stem cells (PSCs) under rapid proliferation requires mechanisms that both suppress replication-driven genome instabilit
A fast 2C-induction method reveals a barrier role of SP2 for totipotency biorxiv.org 2026-07-23 The acquisition of totipotency in vitro remains a major challenge, limiting our understanding of early embryogenesis and its clinical and translationa
A tight balance of anabolic mTORC1 signaling and catabolic autophagic activity regulates zebrafish heart regeneration biorxiv.org 2026-07-23 Zebrafish can regenerate the heart by proliferation of cardiomyocytes.
Collateral effects of lethal management operations: biologging reveals multiscale responses in non-target vervet monkeys biorxiv.org 2026-07-23 Lethal wildlife management operations are typically evaluated through their direct effects on target species, yet their disturbance footprint may exte
Ultrastructure of stemness and differentiated state in Hydra epithelial cells biorxiv.org 2026-07-23 Epithelial cells in Hydra perform an unusual combination of functions: they divide continuously like adult stem cells while simultaneously executing t
From prehistory to present-day: How isolation shaped the distinct genomic makeup of Italian Alpine valleys biorxiv.org 2026-07-23 Historically, the eastern Italian Alps have provided crucial geographic corridors for cultural and genetic exchange between the Mediterranean region a
Fixation Probabilities of Mutant Alleles in an Ecological Context biorxiv.org 2026-07-23 For decades, population geneticists have relied upon a formula derived by Malecot and by Kimura to estimate the fixation probability of mutant alleles
Mobility shapes plasmid GC content evolution biorxiv.org 2026-07-23 Plasmids are frequently AT-rich relative to their bacterial hosts.
Open or closed? Exploring the conformational heterogeneity of human plasminogen across multiple resolution scales biorxiv.org 2026-07-23 Plasminogen (Plg), the zymogen of the fibrinolytic protease plasmin, is a multidomain, conformationally-rich protein that plays a crucial role in targ
Integrated Bioinformatics Analysis of PALB2 Reveals Expression Patterns, Molecular Interactions, and Prognostic Significance in Breast Cancer biorxiv.org 2026-07-23 Background: Partner and Localizer of BRCA2 (PALB2) is a key tumor suppressor gene involved in homologous recombination mediated DNA repair through its
A transparent multicriteria and fuzzy classification approach for genome-based probiotic candidate prioritisation biorxiv.org 2026-07-23 The identification of novel probiotic candidates with potential health-promoting properties remains a major challenge in food biotechnology and increa
MYC/MIZ1 suppression of lysosomal protein degradation drives immune evasion in pancreatic ductal adenocarcinoma biorxiv.org 2026-07-23 The MYC oncoprotein promotes immune evasion of pancreatic ductal adenocarcinoma (PDAC), but the underlying molecular mechanisms are not fully understo
Pulsed priming with the FAK inhibitor narmafotinib enhances both gemcitabine/Abraxane and FOLFIRINOX chemotherapy response in pancreatic cancer biorxiv.org 2026-07-23 Background: Pancreatic ductal adenocarcinoma (PDAC) is a particularly lethal malignancy with few treatment options available.