Iterative Spatial Resolution Enhancement in Imaging Mass Spectrometry via Hydrogel Tissue Expansion and Multimodal Image Fusion biorxiv.org 2026-06-08 The pixel size of imaging mass spectrometry (IMS) is fundamentally limited by several factors, including the diameter of the incident probe and the ra
An imaging framework for nuclei-based three-dimensional cell quantification in intact tissue using phase-contrast X-ray CT biorxiv.org 2026-06-08 Quantifying cells within intact three-dimensional biological specimens remains a major challenge, as standard optical and histological techniques are
USP7 sustains PAX3::FOXO1 enhancer reprogramming and represents a therapeutic vulnerability in Rhabdomyosarcoma biorxiv.org 2026-06-08 Rhabdomyosarcoma (RMS) is the most common soft tissue sarcoma in children and is often associated with dismal outcomes, underscoring the urgent need f
Long-term effects of early-life thermal fluctuations on the cellular stress response and CTmax of zebrafish, Danio rerio biorxiv.org 2026-06-08 Temperatures in aquatic ecosystems have been affected by anthropogenic activities such as agricultural and industrial water use, and climate change ca
Drivers of space use in a large semi-urban feral ungulate under seasonally fluctuating resource conditions biorxiv.org 2026-06-08 Resource scarcity prompts animals to adjust their space use in ways that enhance their survival.
Inhibition of protein tyrosine phosphatase PTP1B function ameliorates pathophysiological deficits in Rett Syndrome biorxiv.org 2026-06-08 Rett syndrome (RTT) is a severe neurodevelopmental disorder in which current therapeutic strategies remain largely focused on providing symptomatic re
Spatial atlas of the ovary identifies molecular events in primordial follicle activation in humans biorxiv.org 2026-06-08 The human ovarian reserve is established prenatally, when granulosa cells encapsulate germ cells to form a pool of quiescent primordial follicles, tha
Riboflavin-sensitized UVA collagen crosslinking produced a controllable, dose-dependent increase in the nanomechanical strength of ex vivo bovine dura mater biorxiv.org 2026-06-08 Background: Dural defects, either from trauma, tumor resection, surgical approaches, genetics, or spontaneously represent a significant clinical chall
Severe feed restriction in a fish: is the positive effects of nucleotide supplementation on growth recovery explained by one-carbon metabolism? biorxiv.org 2026-06-08 Charrs (Salvelinus spp.) are strong candidates for cyclical feed-restriction strategies due to their documented exceptional ability to withstand and r
The ENIGMA MEG Pipeline: Automated cortically localized spectral analysis of multi-site resting state MEG datasets biorxiv.org 2026-06-08 Background Magnetoencephalography (MEG) is a unique technique in human neuroimaging combining high temporal resolution (millisecond or faster) with mo
Cross-presentation of citrullinated antigens drives cytotoxic CD8+ T cell responses in rheumatoid arthritis biorxiv.org 2026-06-08 Rheumatoid arthritis (RA) is an autoimmune synovitis marked by anti-citrullinated protein antibodies (ACPAs) and infiltration of the synovium by activ
Msc1 accumulates at and excludes nuclear pore complexes from nucleus-vacuole junctions. biorxiv.org 2026-06-08 Msc1 is a yeast nuclear envelope (NE) protein that facilitates DNA double-strand break repair.
Intestinal fructose metabolism drives unsaturated fat absorption and synergizes with GLP-1 receptor agonism to promote weight loss biorxiv.org 2026-06-08 High-fat, high-sucrose (HFHS) diets are established risk factors for obesity.
Pubertal maturation and chemotherapy-associated disruption of the pediatric ovary revealed by multimodal single-cell profiling biorxiv.org 2026-06-08 Ovarian tissue cryopreservation enables fertility preservation in females undergoing gonadotoxic therapies, restoring fertility in adults.
Environmental gradients shape climate adaptive phenotypes in Muscovy ducks in Benin with implications for adaptation policy. biorxiv.org 2026-06-08 Climate change threatens livestock-dependent livelihoods across sub-Saharan Africa, yet National Adaptation Programmes of Action (NAPAs) and Nationall
Procedure-Matched Comparison of TNBS/Ethanol Enema and DSS Free-Drinking Models of Ulcerative Colitis in Rats: Immune Endotype Profiling and a Translational Model-Selection Framework biorxiv.org 2026-06-08 Objective: Choosing between TNBS/ethanol enema and DSS free-drinking water protocols for establishing rat ulcerative colitis(UC) models remains a prac
Nanoflow ion-pairing LC-MS for ultra-low-input polar metabolomics and isotope tracing biorxiv.org 2026-06-08 Low-input and single-cell metabolomics remain constrained by the poor retention of polar metabolites in conventional reversed-phase nanoflow LC-MS wor
DDI_single: Single-Sequence-Based Protein Domain Assembly biorxiv.org 2026-06-08 Domains are the basic units of protein structure and function.
MLL3 adaptor function, not methyltransferase catalytic activity, is essential for breast tumor suppression biorxiv.org 2026-06-08 MLL3 (Mixed-Lineage Leukemia 3), also known as KMT2C, is one of the most frequently altered epigenetic regulators in breast cancer.
SMARCA4, STK11, and KEAP1 co-inactivation associates with poor prognosis and upregulation of the TGF-β pathway in lung adenocarcinoma biorxiv.org 2026-06-08 Background Lung adenocarcinoma (LUAD) is clinically and molecularly defined by oncogenic driver mutations, identification of which has led to the deve
A transition to a more efficient attentional strategy facilitates motor learning in the presence and absence of movement-evoked experimental pain. A cross-sectional experimental study. biorxiv.org 2026-06-08 Pain demands attention and can disrupt task-related goals.
GCK-4 regulates apical actin organization and lumen formation in the C. elegans intestine biorxiv.org 2026-06-08 Epithelial tubes are an essential component of many organ systems.
Minimal essential requirements for neural tube self-organisation biorxiv.org 2026-06-08 The reliable generation of diverse cell types in precise proportions is essential for the formation of functional tissues during embryonic development
Attenuated estrogen signaling disrupts placentation and drives trophoblast defects in Down syndrome biorxiv.org 2026-06-08 Proper establishment of the primitive placenta and subsequent tissue homeostasis in the mature placenta are critical for successful pregnancy in human
A Pan-pangenome illuminates complex structural variation and selection in humans, chimpanzees, and bonobos biorxiv.org 2026-06-08 Complete, haplotype-resolved genome assemblies have provided unprecedented insight into the evolution of structurally complex, rapidly evolving region
Who takes the risk to forage? Testing consistent inter-individual behavioural variation in wild vervet monkeys (Chlorocebus pygerythrus) biorxiv.org 2026-06-08 Risk-taking behaviour permeates daily decision-making across many taxa and has significant impact on fitness outcomes.
From topography to connectome: Towards an integrated understanding of the resting brain biorxiv.org 2026-06-08 As the field expands from early research into the human connectome, there has been a fast expansion in the number of analytical approaches to study re
The length and time constants of propagating action potentials biorxiv.org 2026-06-08 Length and time constants are foundational to the study of conduction in neurons and other biological cables but are exactly defined only for passive
Cellular Complexity and Systemic Immune Profiles across Ancestral Diversity in Thailand and Mainland Southeast Asia biorxiv.org 2026-06-08 Mainland Southeast Asia (MSEA) remains under-represented in global immunogenomic references despite its extensive genetic heterogeneity.
Ion channel and receptor-mediated regulation of axonal conduction reliability in sympathetic preganglionic neurons. biorxiv.org 2026-06-08 Sympathetic preganglionic neurons (SPNs) provide the sole spinal output to the peripheral sympathetic nervous system.