Holotomography Reveals Protein Concentration Changes in Cardiac Spheroids under Ischemic Stress biorxiv.org 2026-06-10 Multicellular spheroids better recapitulate native cardiac tissue than two-dimensional systems, preserving cell-cell and cell-matrix interactions and
Structural Evidence for Occupancy-Dependent Inter-Site Coupling in Human Glutathione Synthetase biorxiv.org 2026-06-10 Human glutathione synthetase (hGS) is a negatively cooperative ATP-grasp enzyme that catalyzes the final step in the biosynthesis of glutathione, a tr
NANOG assembles into self-limiting aging micelles that drive a sol-gel transition and modulate DNA dynamics biorxiv.org 2026-06-10 Proteins and nucleic acids form non-Newtonian liquids with complex rheological properties that contribute to their function in vivo.
An Altered Glycome Shapes IgA B-Cell Responses and Gut Immunity During Intestinal Inflammation biorxiv.org 2026-06-10 A healthy gut immune system balances pathogen defense and tolerance to beneficial microbes.
Transient protein phosphorylation promotes disease tolerance to sepsis biorxiv.org 2026-06-10 One of the enduring paradoxes of sepsis is that organs fail despite little evidence of irreversible tissue injury.
Persistent stromal reprogramming defines incomplete mucosal healing and predicts therapeutic response in ulcerative colitis biorxiv.org 2026-06-10 Mucosal healing (MH) is the primary therapeutic endpoint in ulcerative colitis (UC), yet frequent relapses suggest it does not reflect complete tissue
CXCL10-driven STAT3 signaling programs pathogenic CD4+ T cell responses and limits antiviral immunity during arthritogenic alphavirus infection biorxiv.org 2026-06-10 Arthritogenic alphaviruses, including onyong nyong (ONNV), chikungunya virus (CHIKV), and Mayaro (MAYV) viruses, cause acute and chronic inflammatory
Fisetin-mediated MYC restoration improves age-associated decline in macrophage function biorxiv.org 2026-06-10 Immune decline in older adults is associated with increased susceptibility to infection and chronic inflammatory diseases.
Beta cell reactivity defines disease-relevant pancreatic CD8 T cells in type 1 diabetes biorxiv.org 2026-06-10 Type 1 diabetes (T1D) is characterized by immune-mediated destruction of pancreatic beta cells, yet the properties that distinguish disease-associated
Waste oil substrates reshape the black soldier fly larval gut microbiome and biomass composition during bioconversion biorxiv.org 2026-06-10 Black soldier fly larvae (BSFL) are generalist decomposers with promise for converting agricultural and food-processing by-products into value-added b
Transposon insertion sequencing identifies genetic determinants of intrinsic rifamycin resistance in Mycobacterium abscessus biorxiv.org 2026-06-10 Mycobacterium abscessus exhibits intrinsic resistance to many antimicrobial agents, including rifampicin, a frontline anti-tuberculosis drug, severely
Evaluation of antiviral treatments for highly pathogenic avian influenza virus infections in feline species biorxiv.org 2026-06-10 In 2020, highly pathogenic avian influenza (HPAI) isolates from clade 2.3.4.4b emerged in Europe and spread globally, including in bovine hosts in the
Epithelial innate immune sensing of pneumococci is inherently restricted to a small cellular minority across species and infection niches biorxiv.org 2026-06-10 Streptococcus pneumoniae colonises the nasopharynx asymptomatically yet causes life-threatening invasive disease.
Sequential Use of Two Capsule-Targeting Klebsiella Phages Reveals Order-Dependent Efficacy and Distinct Resistance Pathways in vitro and in vivo biorxiv.org 2026-06-10 Bacteriophage cocktails are widely assumed to improve treatment of multidrug-resistant Klebsiella pneumoniae, yet many therapeutic phages target capsu
Most inhibitors with defined yeast targets have limited activity against the microsporidian Nematocida parisii biorxiv.org 2026-06-10 Microsporidia are obligate intracellular parasites that infect diverse animals.
STAT transcription factors regulate host defences and vacuolar integrity during Mycobacterium marinum infection biorxiv.org 2026-06-10 Signal transducers and activators of transcription (STAT) are central regulators of cytokine-mediated immunity in metazoans, yet their ancestral funct
Candidozyma auris utilizes transferrin, but not heme-bound iron for in vivo virulence biorxiv.org 2026-06-10 Candidozyma auris (C.
Functional multi-axis decomposition of the human gut microbiome: an operational definition of eubiosis and dysbiosis, and a clean-reference framework for disease stratification biorxiv.org 2026-06-10 For two decades, gut microbial eubiosis and dysbiosis have resisted operationalisation because diversity-, taxon- and ratio-based criteria fail cross-
A bifunctional coiled-coil protein generates the membrane-within-condensate architecture of the CO2-fixing pyrenoid biorxiv.org 2026-06-10 How membranes are integrated into biomolecular condensates is a fundamental question in cell biology.
Microbiome-Derived RNA Promotes Heritable Defense Against Intracellular Pathogens in Caenorhabditis elegans biorxiv.org 2026-06-10 Intestinal microbiota shape host immunity, but whether they can pre-activate defense against intracellular pathogens remains unclear.
Strand Displacement Activity of Mimiviral Polymerase X Enables Rapid Detection of Sequence-Specific DNA Targets biorxiv.org 2026-06-10 Mimiviral polymerase X, mvPolX, is a repair polymerase that is involved in base excision repair (BER) and carries out the gap-filling function in doub
GABAergic projection from the subiculum to the medial entorhinal cortex in mice and rats biorxiv.org 2026-06-10 The subiculum (SUB) is a major hippocampal output hub that routes information to cortical and subcortical targets, and its long-range projections are
Dynamic trajectory cues drive sequenced integration in approach detectors biorxiv.org 2026-06-10 Detecting approaching objects is essential for survival, and studies across animals have identified neurons and circuits selective for objects that gr
Dynorphinergic neuroadaptations in the islands of Calleja: implications for alcohol use disorder biorxiv.org 2026-06-10 Dysregulation of the dynorphin (DYN) / kappa-opioid receptor (KOR) system is heavily implicated in symptoms of alcohol use disorder (AUD) including ne
BiXformer: A Bidirectional Cross Attention Transformer for Disentangling Inter-Regional Neural Dynamics biorxiv.org 2026-06-10 Advances in high-throughput neural recording technologies enable simultaneous measurement of activity across multiple brain regions in behaving animal
α-Synuclein strain homogeneity in multiple system atrophy clinical subtypes biorxiv.org 2026-06-10 Conformationally distinct 'strains' of -synuclein aggregates are believed to contribute to the clinical and pathological diversity observed among synu
Pathology in resected areas of FDG PET hypometabolism in pediatric epilepsy patients with focal cortical dysplasia biorxiv.org 2026-06-10 Introduction: [18F]fluorodeoxyglucose positron emission tomography (FDG-PET) frequently reveals hypometabolism extending beyond the epileptogenic zone
Lineage-specific chromatin poising enforced by ETS-IRF composite elements determines the divergent interferon responses of plasmacytoid dendritic cells and epithelial cells biorxiv.org 2026-06-10 Plasmacytoid dendritic cells (pDCs) produce robust type I interferons (IFN-I) within hours of viral sensing, while epithelial cells at mucosal surface
Viral load-driven systemic immune exhaustion is an enabler of antibody breadth in HIV infection biorxiv.org 2026-06-10 A broadly neutralizing response to a human immunodeficiency virus (HIV) vaccine is a major goal of the field, yet the determinants of antibody breadth
Circadian Clock Control of Muscle Stem Cells Through Temporal Coordination of Notch and Wnt Signaling biorxiv.org 2026-06-10 The circadian clock regulates stem cell responses during tissue remodeling and repair.
Hemoglobin C is prone to oxidative denaturation, resulting in red blood cell membrane damage in HbSC disease biorxiv.org 2026-06-10 Sickle-hemoglobin-C (HbSC) sickle cell disease is characterized by RBC dehydration (xerocytosis), which promotes polymerization of HbS.
Staurosporine drives non-canonical melanocyte maturation by coupling β-catenin signaling to actin-dependent dendrite remodeling biorxiv.org 2026-06-10 Skin pigmentation relies on the coordinated regulation of melanin production and dendritic morphology to ensure effective pigment distribution.
Flight performance of the highly invasive box tree moth Cydalima perspectalis (Lepidoptera: Crambidae) biorxiv.org 2026-06-10 The multivoltine non-native box tree moth, Cydalima perspectalis (Lepidoptera: Crambidae), was accidentally introduced in several continents and exhib
Outbred Drosophila populations reveal diet-dependent genetic effects on development time biorxiv.org 2026-06-10 Metabolic effects of genetic variation often depend on diet, yet the loci underlying diet-dependent developmental responses remain incompletely define
Gene loss under constant cold reveals "natural knockout" loci in Antarctic notothenioid fishes biorxiv.org 2026-06-10 Abstract Background Gene loss is a major but often underappreciated mode of genome evolution.
The Conductome: A Bayesian Classifier Approach to Predicting and Understanding Behaviour biorxiv.org 2026-06-10 Predicting and understanding behaviour is a primary objective of many disciplines, especially human behaviour, as it is the cause of many of the world
Electroadhesion of polymer networks by polycation interfacial bridging: sticky electrophoresis, ionic complexation, and chain entanglement biorxiv.org 2026-06-10 An e-GLUE is a polymer network containing interpenetrating polycations, which can bond the anionic network of mucosa through interfacial polycation br
Biocompatible designated Resin-3D-printed polymers exhibit reproductive toxicity prevented by Parylene-C biorxiv.org 2026-06-10 Resin three-dimensional (3D) printing is an increasingly popular manufacturing and prototyping method used to create microphysiological systems (MPS),
SPARQ-MI leverages end-to-end spatial single-cell analysis of the tumor microenvironment biorxiv.org 2026-06-10 Detailed spatial analysis of the tumor micro-environment (TME) through multiplexed fluorescence imaging requires quantitative image-processing and dat
Comparative Molecular Dynamics Characterization of Hair Keratin Unfolding Mechanics biorxiv.org 2026-06-10 Keratin proteins are fundamental structural components of hair fibers, contributing to their mechanical resilience, elasticity, and fracture resistanc
Dietary omega-6 arachidonic acid and omega-3 docosahexaenoic acid supplementation differentially impact skeletal muscle inflammaging in mice biorxiv.org 2026-06-10 Aging is associated with a gradual and progressive decline in skeletal muscle mass and strength known as sarcopenia, which has been attributed to chro
Neural Innervation Invigorates Yolk Sac Biological Functions beyond Nutrient Reservoir during Zebrafish Embryo Development biorxiv.org 2026-06-10 The zebrafish yolk sac (YS) is traditionally viewed as a nutrient reservoir.
Neuro-glial lipid imbalance in a Drosophila model of Amyotrophic Lateral Sclerosis 8 biorxiv.org 2026-06-10 Membrane Contact sites (MCS) have emerged as physiologically relevant zones that coordinate inter-organelle communication and cellular function.
Use of a plasmid containing a dual gene reporter system to assess the cell hydrophobicity of Listeria monocytogenes biorxiv.org 2026-06-10 Listeria monocytogenes causes listeriosis in humans and animals and contaminates prepared food by attaching to food processing environments.
SPAFESTWDILK, a plant-derived dodecapeptide from Zingiber officinale, as a predicted inhibitor of the MDM2-p53 interaction: computational discovery and multi-method evaluation biorxiv.org 2026-06-10 The MDM2-p53 protein-protein interaction is a validated oncology target, yet no food-derived linear peptide has been documented to engage the canonica
Active Learning-Guided Peptide Design for Modulating Condensate Properties upon Recruitment biorxiv.org 2026-06-10 The physical properties of biomolecular condensates, which form through phase separation, are central to their organisation and function and are incre
Estrogen-Deficiency Degrades Left Ventricular Diastolic Function and Energy Metabolism in Hypertensive Female Mice biorxiv.org 2026-06-10 Aims: Heart failure (HF) due to diastolic dysfunction (DD) but normal left ventricular (LV) ejection fraction (EF) is termed HF with preserved EF (HFp
Capsinoids (non-pungent TRPV1 agonists) promote mild hypothermia and improved outcome following stroke in aged mice biorxiv.org 2026-06-10 Background and Purpose: Mild hypothermia provides potent neuroprotection in experimental ischemic stroke, however, its implementation in awake stroke
A microbial-sensory axis drives Pseudomonas aeruginosa-induced mechanical itch biorxiv.org 2026-06-10 Cutaneous bacterial infections frequently elicit severe pruritus, prominently featuring alloknesis, a pathological state where innocuous touch provoke
Cortical E-fields of deep brain stimulation in Parkinson's disease patients exceed typical E-field magnitudes of transcranial electrical stimulation biorxiv.org 2026-06-10 Background: Deep brain stimulation (DBS) is an established and effective intervention for Parkinson's disease.