Dynamic Patterns of Nuclear Transcription Factor Abundance in Plant Basal Immunity Revealed by Spatial Proteomics of Arabidopsis Nuclei biorxiv.org 2026-07-09 O_LIThe control of amount of nuclear proteins is fundamental in regulating plant gene expression, but the mechanisms of quantitative dynamics of the n
NLR from soybean Rsv1 locus confers broad-spectrum resistance to soybean mosaic virus G1-G7 strains by recognizing viral P3 protein biorxiv.org 2026-07-09 Nucleotide-binding leucine-rich repeat (NLR) immune receptor genes are of significant value in disease resistance breeding and the control of viral di
A new genome-scale model enables prediction of cancer metabolic dependencies biorxiv.org 2026-07-09 Cancer cells rewire metabolism to support proliferation. Intriguingly, divergent metabolic choices are made to attain this common goal.
Connexin 30 mutation rescues high-frequency hearing, reduces gap junctional coupling and alters potassium currents in cochlear Deiters cells biorxiv.org 2026-07-09 The early-onset, high-frequency hearing loss phenotype of CD-1 mice is rescued by the A88V mutation of the connexin 30 gap-junctional protein, despite
Challenges and Solutions in Quantifying Brain β-Hydroxybutyrate (BHB) with 1H-MRS Following Oral Keto-Ester Consumption biorxiv.org 2026-07-09 Purpose: {beta}-hydroxybutyrate (BHB), a ketone body and alternative cerebral energy substrate, can be measured in vivo using J-difference edited prot
Hippocampal CA3 forms a two-layer network of molecularly distinct cell types in mice and humans biorxiv.org 2026-07-09 Associational memory is critically dependent on the CA3 recurrent circuit, at the centre of the hippocampal system.
Exposure to P. falciparum and common cold viruses shape vaccine responses in early life biorxiv.org 2026-07-09 Vaccine immunogenicity is consistently lower in low-income countries than in high-income settings, yet the factors driving this disparity remain incom
Ecological connectivity modelling with WebAssembly biorxiv.org 2026-07-09 Circuit theory has been successfully applied to ecological connectivity modelling, notably via the Circuitscape software, which is typically run local
Data-driven forecasts of regional arrivals of non-native vertebrates worldwide biorxiv.org 2026-07-09 Aim: To forecast near-future arrivals of non-native terrestrial and freshwater vertebrates at the regional level.
Two-tower models for genomic prediction of reproductive outcomes and sex-specific fertility liabilities: simulation insights biorxiv.org 2026-07-09 Many biological characteristics arise by interactions between more than one biological organism or unit.
Improving coral oxidative stress assessments through compartment-specific lipid peroxidation measurements and increased methodological standardization biorxiv.org 2026-07-09 Lipid peroxidation (LPO) is widely used as a biomarker of oxidative stress in coral bleaching research, yet its measurement remains poorly standardize
Coated Bacterial Enzymes: A one-step approach for enzymatic purification and immobilization biorxiv.org 2026-07-09 The purification of industrial enzymes typically relies on costly, multi-step chromatographic protocols.
Variational Autoencoder-enabled High-throughput Drug Screening for HIV Latency Modulators predicted through Noise in Gene Expression biorxiv.org 2026-07-09 Due to its ability to establish a pool of undetectable and latently infected cells that can initiate viral production through random reactivation, a c
Directed evolution of the Fe-nitrogenase for CO2 reduction to hydrocarbons biorxiv.org 2026-07-09 The iron (Fe) nitrogenase drives bacterial methane (CH4) formation by converting carbon dioxide (CO2) to CH4 in a single enzymatic step.
Expanding Microgel Parameters to Model the Tumor Microenvironment and Examine Temozolomide Resistance in Glioblastoma biorxiv.org 2026-07-09 Glioblastoma (GBM) is a highly aggressive brain tumor with a five-year survival rate of less than 5%.
Device-embedded accelerometry complements neural signals for tracking parkinsonian motor states biorxiv.org 2026-07-09 Adaptive deep brain stimulation (aDBS) relies on physiological biomarkers to infer motor state and guide therapeutic stimulation in Parkinson's diseas
Vibration's frequency and intensity for optimal setup for enhancement bone response in small rodents: A systematic review and Bayesian network meta-analysis biorxiv.org 2026-07-09 Low-intensity vibration (LIV) is a non-invasive mechanical stimulus capable of regulating skeletal adaptation and cellular signaling pathways involved
A non-enzymatic role for METTL3 as an Androgen Receptor co-regulator that promotes prostate cancer proliferation. biorxiv.org 2026-07-09 Metastatic prostate cancer (PCa) continues to be a major cause of death in males, despite advances in treatment.
An immunocompetent osteoblastic model of mammary cancer bone metastasis established by syngeneic intratibial injection of PyMT mammary carcinoma cells in FVB/N mice biorxiv.org 2026-07-09 Osteoblastic bone metastasis, in which disseminated tumor cells drive net bone formation, is a clinically distinct and mechanistically underexplored f
Predicting subclonal TP53 mutations from tumor spatial transcriptomics data using a graph convolutional neural network biorxiv.org 2026-07-09 Spatial transcriptomics (ST) has revolutionized our understanding of tumor biology but inherently lacks information on the upstream somatic driver mut
Correlation of OCT-Based Radiomic Signatures With Dose-Associated Radiation Response in Tumor Spheroids biorxiv.org 2026-07-09 Background: Three-dimensional tumor spheroids are an established radiobiology model, but scalable, reproducible readouts of dose-dependent radiation r
Leveraging Homologous Recombination Deficiency via the Repositioned Prodrug CB1954 biorxiv.org 2026-07-09 Homologous recombination deficiency (HRD) is an actionable vulnerability found in a substantial fraction of human cancers, yet current HRD-directed th
Making the most out of it: shallow genome-skimming possibilities for the systematics of prickly lineages of Solanum (Solanaceae) biorxiv.org 2026-07-09 Premise: Genome skimming (GS) is a cost-effective approach for plant phylogenomics, but its ability to recover informative datasets from different gen
Characterisation of the new microalgal protein xATPA related to the F-type ATP synthase α subunit, from the ecosystem to the molecule biorxiv.org 2026-07-09 Microalgal metabolism relies on their chloroplasts, and involves both nucleus and plastidial-encoded proteins of various evolutionary origins.
miR319 promotes de novo shoot regeneration by repressing LsTCP4 in lettuce biorxiv.org 2026-07-09 Plant regeneration is a major determinant of transformation and genome-editing efficiency, yet the endogenous regulatory networks controlling regenera
Characterising AlphaFold 3s ability to predict T cellantigen specificity biorxiv.org 2026-07-09 T cells are a key part of the adaptive immune system.
Domain-specific proteome remodeling defines mouse myelin aging biorxiv.org 2026-07-09 Myelin, once regarded as a static insulating structure, is now recognized as a dynamic component of the nervous system, capable of remodeling in respo
A pooled image-based CRISPR screen identifies EAF1 as a T. gondii modulator of ESCRT subversion. biorxiv.org 2026-07-09 Intracellular pathogens remodel host cells by redirecting cellular machinery to the host-pathogen interface.
Tissue-specific consequences of impaired RNA surveillance converge on mitochondrial homeostasis biorxiv.org 2026-07-09 RNA surveillance pathways maintain transcriptome integrity by eliminating aberrant, excess, and non-functional RNAs, yet it remains unclear whether di
Aclarubicin disrupts RNA polymerase II progression at replication-coupled histone genes biorxiv.org 2026-07-09 Anthracyclines are highly effective chemotherapeutic agents that cause DNA and chromatin damage.
A Sac7-Rho1 axis at the plasma membrane controls clathrin-independent endocytosis biorxiv.org 2026-07-09 In eukaryotes, our understanding of clathrin-independent endocytosis (CIE) lags far behind that of clathrin-mediated endocytosis (CME).
Mechanical strain of the intestinal epithelium directs absorptive lineage maturation biorxiv.org 2026-07-09 The intestinal epithelium is continuously subjected to a variety of mechanical forces, including extrinsic peristaltic contractions and intrinsic tens
SOLAR Mediates Lysosomal Membrane Repair and Redox Defense biorxiv.org 2026-07-09 Lysosomal damage is increasingly recognized as a hallmark of aging.
Measurement of a panel of 21 steroids in a quantitative assay in human plasma, adipose tissue, and fecal samples using ultra-high-performance liquid chromatography-tandem mass spectrometry biorxiv.org 2026-07-09 Comprehensive detection of steroids, beyond the limited panels typically analyzed in clinical chemistry laboratories, has become increasingly importan
Estimating Muscle Parameters via Hierarchical Bayesian Inference biorxiv.org 2026-07-09 Musculoskeletal models are widely used to relate muscle mechanics to movement patterns in biomechanics.
Photopatterned spatiotemporal organisation and in situ differentiation of 3D human cortical networks biorxiv.org 2026-07-09 Modelling human cortical microcircuitry in vitro requires platforms that recapitulate both the compositional complexity and spatial architecture of de
qg: Configuration-Driven, Multi-Vendor Acquisition Queue Generation with Reproducible Run-Order and QC Control for Mass Spectrometry biorxiv.org 2026-07-09 Mass spectrometry laboratories must turn lists of submitted samples into acquisition queues.
Protein hydration and druggability biorxiv.org 2026-07-08 A priori assessment of target proteins' druggability remains an unsolved problem in the field of drug development.
Emergence of large-scale polar microtubule swarms for dense molecular transport biorxiv.org 2026-07-08 Microtubules (MTs) and their motor proteins collectively harness chemical energy to generate mechanical work, driving some of the most coordinated sel
Molecular Structure, DNA Binding, and Photophysical Properties of SYTOX Orange and SYTOX Green biorxiv.org 2026-07-08 Fluorescent dyes are critical to visualizing nucleic acids in many applications.
SARS-CoV-2 membrane protein conformations induce distinct membrane curvatures biorxiv.org 2026-07-08 The assembly and budding of enveloped viruses requires thousands of membrane proteins to collectively remodel host-cell membranes into highly curved v
Graph neural network modeling of receptor interaction kinetics from single-molecule imaging data biorxiv.org 2026-07-08 Single-molecule (SM) imaging (SMI)-based approaches have the powerful ability to capture receptor interactions, which are necessary for cell signaling
Combined AMPK activation and ghrelin ameliorate cancer cachexia through complementary effects on energy homeostasis, inflammation, and wasting biorxiv.org 2026-07-08 BackgroundCancer-associated cachexia is characterized by progressive loss of skeletal muscle and adipose tissue driven by systemic inflammation and me
4D spatial transcriptomics reveals nodule identity emerges through stacked parallel developmental programs biorxiv.org 2026-07-08 Legume root nodules enable symbiotic nitrogen fixation through the development of specialized cells that accommodate nitrogen-fixing bacteria intracel
A family of RRM-1 RNA binding proteins enables cold adaptation and environmental resilience in Bacteroides biorxiv.org 2026-07-08 Bacteria use post-transcriptional regulatory mechanisms to rapidly adjust gene expression during environmental change.
Conserved herpesvirus protein kinase (CHPK)-mediated phosphorylation of viral proteins associated with nucleocytoplasmic trafficking during natural infection biorxiv.org 2026-07-08 The conserved herpesvirus protein kinase (CHPK) is encoded by all members of the Orthoherpesviridae and contributes to replication in cell culture but
Identification of Viral and Cellular Proteins in Proximity to the HSV-2 pUL16 Tegument Protein in Infected Cells biorxiv.org 2026-07-08 Orthologs of the herpes simplex virus (HSV) pUL16 tegument protein are conserved throughout the Orthoherpesviridae family.
Fluorescently guided workflow with rationally engineered 5' ligation adapters for high-sensitivity and low-bias small RNA sequencing biorxiv.org 2026-07-08 MicroRNAs (miRNAs) act as key regulators of gene expression across diverse cellular processes, and their precise quantification can provide unique ins
Circadian temperature compensation is intrinsically linked with metabolism and redox signaling biorxiv.org 2026-07-08 Circadian rhythms are a universal property of organisms, and a defining property of these oscillators is the ability to maintain a precise period at d
Molecular mechanisms of E-Syt-mediated stress resistance biorxiv.org 2026-07-08 Membrane contact sites (MCS) between the endoplasmic reticulum (ER) and the plasma membrane (PM) enable direct intermembrane exchange of signals and m