Molecular Structure, DNA Binding, and Photophysical Properties of SYTOX Orange and SYTOX Green biorxiv.org 2026-07-08 Fluorescent dyes are critical to visualizing nucleic acids in many applications.
SARS-CoV-2 membrane protein conformations induce distinct membrane curvatures biorxiv.org 2026-07-08 The assembly and budding of enveloped viruses requires thousands of membrane proteins to collectively remodel host-cell membranes into highly curved v
Graph neural network modeling of receptor interaction kinetics from single-molecule imaging data biorxiv.org 2026-07-08 Single-molecule (SM) imaging (SMI)-based approaches have the powerful ability to capture receptor interactions, which are necessary for cell signaling
Combined AMPK activation and ghrelin ameliorate cancer cachexia through complementary effects on energy homeostasis, inflammation, and wasting biorxiv.org 2026-07-08 BackgroundCancer-associated cachexia is characterized by progressive loss of skeletal muscle and adipose tissue driven by systemic inflammation and me
4D spatial transcriptomics reveals nodule identity emerges through stacked parallel developmental programs biorxiv.org 2026-07-08 Legume root nodules enable symbiotic nitrogen fixation through the development of specialized cells that accommodate nitrogen-fixing bacteria intracel
A family of RRM-1 RNA binding proteins enables cold adaptation and environmental resilience in Bacteroides biorxiv.org 2026-07-08 Bacteria use post-transcriptional regulatory mechanisms to rapidly adjust gene expression during environmental change.
Conserved herpesvirus protein kinase (CHPK)-mediated phosphorylation of viral proteins associated with nucleocytoplasmic trafficking during natural infection biorxiv.org 2026-07-08 The conserved herpesvirus protein kinase (CHPK) is encoded by all members of the Orthoherpesviridae and contributes to replication in cell culture but
Identification of Viral and Cellular Proteins in Proximity to the HSV-2 pUL16 Tegument Protein in Infected Cells biorxiv.org 2026-07-08 Orthologs of the herpes simplex virus (HSV) pUL16 tegument protein are conserved throughout the Orthoherpesviridae family.
Fluorescently guided workflow with rationally engineered 5' ligation adapters for high-sensitivity and low-bias small RNA sequencing biorxiv.org 2026-07-08 MicroRNAs (miRNAs) act as key regulators of gene expression across diverse cellular processes, and their precise quantification can provide unique ins
Circadian temperature compensation is intrinsically linked with metabolism and redox signaling biorxiv.org 2026-07-08 Circadian rhythms are a universal property of organisms, and a defining property of these oscillators is the ability to maintain a precise period at d
Molecular mechanisms of E-Syt-mediated stress resistance biorxiv.org 2026-07-08 Membrane contact sites (MCS) between the endoplasmic reticulum (ER) and the plasma membrane (PM) enable direct intermembrane exchange of signals and m
miR-378a Controls Cardiomyocyte Metabolism and Angiogenic Signaling biorxiv.org 2026-07-08 AimsWhile the muscle-enriched microRNA-378a (miR-378a) has been implicated in cardiac hypertrophy and stress responses, its role in maintaining cardio
Suspension-Based Human Esophagoids Recapitulate WNT2B-Dependent Regulation of Esophageal Basal Progenitors biorxiv.org 2026-07-08 Background & AimsThe human esophagus undergoes a tightly regulated developmental program, transitioning from a simple columnar epithelium in early
Defining biologically directed therapy target signatures in urothelial carcinoma: A transcriptomic framework for precision therapy biorxiv.org 2026-07-08 IntroductionNectin-4 targeting antibody-drug conjugate (ADC) enfortumab vedotin (EV), in combination with pembrolizumab, is the first-line treatment f
A genetic toolkit to reduce wheat immunogenicity and incidence of celiac disease biorxiv.org 2026-07-08 Celiac disease (CeD) is an immune-mediated condition triggered by wheat gluten in genetically predisposed individuals.
Complete elucidation and heterologous reconstruction of the biosynthetic pathway of camptothecin biorxiv.org 2026-07-08 Camptothecin derivatives are first-line anticancer drugs used worldwide for the treatment of diverse malignant tumors.
Decoding Spatial Programs in Human Glioblastoma Through Surprisal Information-Theoretical Analysis biorxiv.org 2026-07-08 Glioblastoma (GBM) is a highly heterogeneous and invasive brain tumor in which complex interactions among tumor cells, immune cells, and neurons shape
Comparative characterization of Cas12a2 orthologs identifies high-activity nucleases for programmable cell elimination biorxiv.org 2026-07-08 Cas12a2 CRISPR nucleases, including SuCas12a2, have been shown to have extensive collateral activity towards RNA, ssDNA, and dsDNA.
Ionic Exposure History Shapes Inner Nuclear Membrane Voltage and Chromatin Texture Responses biorxiv.org 2026-07-08 While bioelectricity is increasingly recognized as an important regulator of cell function and morphogenesis, the field has almost exclusively focused
Relapse-founding cancer persister cells in follicular lymphoma biorxiv.org 2026-07-08 Follicular lymphoma (FL) is an incurable, prototypical relapse-remitting cancer, implying the existence of therapy-persistent cells that survive front
LUstiGE, Light responsive Ustilago maydis Gene Expression: Optogenetic control of morphogenesis and pathogenesis in the corn fungal pathogen Ustilago maydis biorxiv.org 2026-07-09 The basidiomycete Ustilago maydis is a well-characterized model organism for studying pathogen-host interactions and of great interest for a broad spe
The Boolean Breast-Cancer Network (BBCN): structural controllability of cell-fate signalling and a bistable resistance-apoptosis switch biorxiv.org 2026-07-09 We model a breast tumour as a Boolean network of signalling pathways and pose therapy as a control problem: find minimal, druggable interventions that
Overinflation and overconcentration: why Cauchy perturbation kernels are the right choice for ABC-SMC biorxiv.org 2026-07-09 Approximate Bayesian computation sequential Monte Carlo (ABC-SMC) propagates its particles with a perturbation kernel, and with the standard Normal ke
Overnight Th1 immune activation predicts the cortisol awakening response biorxiv.org 2026-07-09 The cortisol awakening response (CAR) marks the transition from rest to wake phase by a sharp increase in cortisol levels upon awakening in the mornin
Spatial transcriptomic programs relate to spectrolaminar rhythms across macaque cortex biorxiv.org 2026-07-09 The laminar organization of cortical dynamics is thought to reflect underlying cell-type architecture, but this relationship has not been resolved acr
Primate Astrocyte Evolution Controls the Tempo of Neuronal Development biorxiv.org 2026-07-08 Prolonged neuronal maturation, also referred to as neoteny, constitutes a hallmark of human brain evolution.
Disrupted myelin homeostasis in grey matter of Alzheimer's disease biorxiv.org 2026-07-08 Alzheimer's disease (AD) is characterized not only by amyloid-beta; plaques, tau neurofibrillary tangles and associated neuronal loss, but also by alt
Regulation of protein abundance in neurons by selective translation of 3'UTR isoforms biorxiv.org 2026-07-09 The precise regulation of protein synthesis is essential for cellular function and survival.
The endothelial scavenger receptor stab2 is required for proper hematopoietic stem and progenitor cell development in the fetal blood stem cell niche biorxiv.org 2026-07-08 Hematopoietic stem and progenitor cell (HSPC) niches support lifelong production of blood and immune cells.
Inter-population connectivity of southern elephant seals and the likely intra-species transmission pathways of high pathogenicity avian influenza biorxiv.org 2026-07-08 High Pathogenicity Avian Influenza (HPAI) H5N1 clade 2.3.4.4b has spread beyond birds to affect seals across the Southern Ocean and sub-Antarctic regi