Synthetic Fibrous Hydrogels as Minimal Systems to Modulate Cell Migration Modes in 3D biorxiv.org 2026-06-02 Cell migration in three-dimensional (3D) environments is highly plastic and regulated by extracellular matrix (ECM) cues.
Physics-guided design of intrinsically disordered proteins biorxiv.org 2026-06-02 Intrinsically disordered protein regions (IDPs) are found across the tree of life and characterized by the lack of a stable 3D fold, encoding function
Hashi: Bridging Statistical Model Derived 1D Microstate Encodings and Protein 3D Structural Ensembles biorxiv.org 2026-06-02 The functioning of proteins is intimately linked to the conformational states they sample within the native ensemble.
Fingertip real contact area scales quadratically with input voltage in electrostatic actuation biorxiv.org 2026-06-02 Touchscreens have become the dominant interface in consumer electronics, yet interactions with them remain primarily visual.
A self-consistent model for phase separation and active processes in biomolecular condensates biorxiv.org 2026-06-02 Biomolecular condensates are thought to play a pivotal role in cellular organization by regulating biochemical reactants in space and time.
Index-agnostic oblique plane light sheet microscopy of centimetre-scale cleared tissues at subcellular resolution biorxiv.org 2026-06-02 We present cleared-tissue direct-view oblique plane microscopy (CtDvOPM), which enables optically sectioned subcellular resolution imaging of centimet
Morphology-robust quantification of subcellular organization in complex cells biorxiv.org 2026-06-01 Quantitative analysis of subcellular protein organization is often confounded by variation in cell morphology, limiting the identification and interpr
Cell-type Specific Alteration of Dicer1 Accelerates Tumor Progression in Mouse Models of KRAS-driven Lung Adenocarcinoma biorxiv.org 2026-06-01 MicroRNAs (miRNAs) have been widely implicated in cancer initiation and progression, yet examination of the effects of global miRNA disruption on thes
Engineering Biosensors to Enhance Monoterpene Indole Alkaloid Production in Yeast biorxiv.org 2026-06-01 Monoterpene Indole Alkaloids (MIAs) are a diverse family of plant natural products with various medicinal applications.
Transcriptomic and proteomic responses to gas vesicle collapse in native and engineered bacterial systems biorxiv.org 2026-06-01 Gas vesicles (GVs) are air-filled protein nanostructures produced by microbes to regulate buoyancy and have emerged as powerful tools in biomedical im
Extracellular vesicles from Manila clam (Ruditapes philippinarum): tailored isolation from hemolymph and insights into water-derived vesicles biorxiv.org 2026-06-01 Extracellular vesicles (EVs) are evolutionarily conserved mediators of intercellular communication released by cells into biological fluids and the ex
Negative HIV serology in perinatally infected children after early treatment and viral suppression: an exploratory analysis of immune correlates biorxiv.org 2026-06-01 Undetectable HIV-specific antibodies in early-treated children with confirmed infection correlate with low viral reservoir and may identify those elig
mRNA-Encoded TLR5 Agonist as an Immune Adjuvant biorxiv.org 2026-06-01 Activation of innate immunity enhances adaptive immune responses and underlies the mechanism of action of vaccine adjuvants.
Unbiased identification of responding T cell clones from longitudinal repertoire sequencing with CloneSearch biorxiv.org 2026-06-01 T cells activate and expand upon interaction with cognate antigen, derived from pathogens or mutated proteins.
Systematic Comparison of Indicator and Pathogenic Viruses Using High-Throughput qPCR Identifies Pepper Mild Mottle Virus as a Robust Indicator of Virus Removal in Wastewater Treatment biorxiv.org 2026-06-01 The selection of appropriate viral indicators for evaluating wastewater treatment performance remains challenging because candidate markers have rarel
A replication hijacking mechanism for Tn3-family replicative transposition biorxiv.org 2026-06-01 Transposition of all classes of transposable elements generates DNA intermediates that must be processed by the host to be effective.
Hypertension-mediated cardiac fibrosis is a mechanical process initiated by smooth muscle cells biorxiv.org 2026-06-01 Hypertension represents the most prevalent chronic cardiovascular condition, typically culminating in pathological cardiac remodeling characterized by
Immune context unmasks regulatory effects of Neanderthal and Denisovan introgression biorxiv.org 2026-06-01 Neanderthal and Denisovan introgression have left a pervasive footprint in the human genome, yet its regulatory consequences remain poorly understood.
New approaches to detecting and characterizing introgression in large species trees biorxiv.org 2026-06-01 Many current phylogeny-based methods to detect introgression use samples of species-quartets to detect asymmetries in gene tree frequencies.
Efficient base editing and development in human embryos without chromosomal alterations biorxiv.org 2026-06-01 Cas9-based tools enable the introduction of genetic lesions to investigate DNA repair outcomes and edit the genome at disease-relevant loci.
Histone succinylation directly inhibits Jumonji domain demethylases and stabilizes repressive chromatin states biorxiv.org 2026-06-01 Herein we uncover a relationship between histone succinylation and Jumonji (JmjC) domain-containing histone demethylases.
Targeting lncRNA JINR1 with programmable Circular Active Nano DNAzyme (CANDe) suppresses Japanese Encephalitis Virus infection biorxiv.org 2026-06-01 Oligonucleotide therapeutics such as antisense oligonucleotides (ASOs) and small interfering RNAs (siRNAs) enable sequence-specific gene silencing but
Hepatic Acetate is an Essential Fuel for the Heart during Energy-Deprived States biorxiv.org 2026-06-01 Energy supply is fundamental to cardiac performance, yet the mechanisms by which the heart adapts to nutrient scarcity remain incompletely understood.
High-density Lipoprotein Inspired Lipid Nanoparticles for Systemic RNA delivery to the Brain biorxiv.org 2026-06-01 Systemic delivery of nucleic acids to the central nervous system (CNS) remains fundamentally limited by the blood-brain barrier (BBB) and the lack of
Monju: Multi-criteria clustering in single-cell omics biorxiv.org 2026-06-01 Clustering is a fundamental step in single-cell omics analysis.
Do AI Structure Predictors Capture Bound-State Disorder? A Benchmark on Fuzzy Protein Complexes biorxiv.org 2026-06-01 Fuzzy protein complexes, in which an intrinsically disordered protein (IDP) retains conformational disorder upon binding, pose a fundamental challenge
DropSynth-Gold: Golden Gate Assembly in Emulsions Extends Multiplexed Gene Libraries to Greater Lengths biorxiv.org 2026-06-01 The ability to synthesize longer genes at scale remains a central challenge in multiplexed gene synthesis.
Integrated valorisation of PET and xylose using the oleaginous microorganisms Yarrowia lipolytica and Rhodococcus jostii biorxiv.org 2026-06-01 The increasing accumulation of polyethylene terephthalate (PET) waste has prompted the development of sustainable biotechnological strategies for its
Vinculin organises apical Transcellular Actin Fibres to coordinate collective migration biorxiv.org 2026-06-01 Collective cell migration is coordinated by adherens junctions (AJs) which serve both as structural links between the actin cytoskeleton of adjacent c
Co-delivered PD-L1 rescues the protective efficacy mediated by an AAV-expressed HIV-1 bNAb biorxiv.org 2026-06-01 Adeno-associated virus (AAV)-delivered anti-HIV-1 broadly neutralizing antibodies (bNAbs) could prevent and treat HIV-1 infection but are limited by h