Attenuation of Inflammatory Cytokines by Selective Vagal Motor Stimulation via Silicone Spiral Nerve Cuff biorxiv.org 2026-06-14 Objectives To determine how selective optogenetic vagus nerve stimulation (VNS) of distinct axonal subpopulations modulates systemic inflammatory cyto
Structural Analysis of Prostate Cancer N-Glycans Using Graph-Based Structural Metrics biorxiv.org 2026-06-14 The N-linked glycans are structurally complex carbohydrate modifications that regulate protein folding, immune recognition, and cellular signaling, an
Cellfm-datasets: A Unified Data Infrastructure for Single-Cell and Spatial Transcriptomics Foundation Model Pretraining biorxiv.org 2026-06-14 Large-scale cell foundation models are increasingly limited not only by model architecture, but also by the data infrastructure required to repeatedly
Systematic AI-Driven Drug Repurposing via Clinical Trial Data Mining: A Framework and Six Cross-Therapeutic Case Studies. biorxiv.org 2026-06-14 Drug repurposing, the application of approved or shelved compounds to new therapeutic indications, offers a cost- and time-efficient alternative to de
APpar: automated action potential parameter analysis software for reproducible electrophysiological measurements in neurons biorxiv.org 2026-06-14 Quantitative analysis of action potential (AP) waveforms is central to studies of neuronal excitability, ion channel function, disease mechanisms, and
Improving All-Atom Molecular Dynamics Models for Quantitative Prediction of Nanopore Blockade Current biorxiv.org 2026-06-14 All-atom molecular dynamics has become an indispensable tool in development of nanopore sensors of biological information.
Effects of oxidative stress and aging on nerve, muscle, and synapse in a male-specific abdominal neuromuscular junction in Drosophila biorxiv.org 2026-06-14 Defects in Drosophila Cu2+/Zn2+ superoxide dismutase (encoded by the gene Sod1) lead to elevated oxidative stress and a drastically shortened lifespan
Lysosomal ion homeostasis drives delayed hair cell death after aminoglycoside uptake. biorxiv.org 2026-06-14 Aminoglycoside ototoxicity has been widely reported and remains an important public health issue.
Prediction of parsimonious and temporally sensitive sets of cell fate engineering transcription factors with IMCell biorxiv.org 2026-06-14 Transcription factor (TF) cocktails used in cell identity reprogramming protocols have largely been developed from experimental approaches.
A Forward Genetic Screen in Caenorhabditis elegans for Genes that Modulate α-synuclein-Induced Neurodegeneration through the Mitochondrial UPR biorxiv.org 2026-06-15 Overexpression of -synuclein (-syn), an inherently disordered protein, triggers chronic activation of the mitochondrial unfolded protein response (UPR
Hypermutability of ultraconserved histone genes and its contribution to human disease biorxiv.org 2026-06-14 Histone genes are among the most evolutionarily conserved sequences in eukaryotes, reflecting their essential role in nucleosome architecture.
Bioclimatic variables influence the strength of purifying selection on mitochondrial DNA in an avian clade (Aves: Piciformes) biorxiv.org 2026-06-14 Mitochondrial loci were for long considered as markers of choice to reconstruct phylogenies.
Identifying transcriptomic bias across developmental shifts in insects biorxiv.org 2026-06-14 Background Synonymous mutations, once considered neutral, can affect translation efficiency through mRNA folding and splicing, generating codon usage
Evolutionary Recoding of Olfactory Sensory Neurons biorxiv.org 2026-06-14 Odorant receptors (Ors) are the interface between an animal's nervous system and its olfactory environment.
Organelle Capture, Lineage-Specific Genomic Responses, and the Lability of Dioecy in Amaranthus biorxiv.org 2026-06-14 The transition from combined to separate sexes in plants drives genomic change, from recombination suppression at sex-determining loci to shifts in se
Morphological shifts consistent with the island syndrome in land-bridge island birds biorxiv.org 2026-06-14 Land-bridge islands are former mainland areas isolated by post-glacial sea-level rise (<15,000 years) and the most common island type.
Transposable elements as evolutionary substrates of proteindisorder in the human proteome biorxiv.org 2026-06-14 Intrinsically disordered regions (IDRs) are central contributors to protein function, evolution and human disease, yet the evolutionary routes that se
TopoMIL: Topology Improves Multiple Instance Learning in Diagnostic Microscopic Images biorxiv.org 2026-06-14 Microscopic images of cells and tissues are central to disease diagnosis.
A novel machine-learning classification model detects oxidative fiber type transitions in a rabbit model of cerebral palsy biorxiv.org 2026-06-14 The distribution of slow- and fast-twitch fiber types in a skeletal muscle heavily influences its physiology.
Genetic analysis of maize seedling root traits under chilling highlights their importance for early field development biorxiv.org 2026-06-14 Characterizing the genetic basis of root system architecture and its role in early plant development is essential for developing maize varieties with
Cardiomyocytes Undergo a Mesenchymal-Like Fate Transition in Myocardial Fibrosis biorxiv.org 2026-06-14 BACKGROUND Myocardial fibrosis, a pathological hallmark of adverse cardiac remodeling and heart failure, has been conventionally attributed to the act
Mutation count and mutation profile analyses of single-nucleotide variants in single human colon crypts biorxiv.org 2026-06-14 Genetic heterogeneity due to the accumulation of mutations in normal tissues can increase cancer risk and be an important factor in many age-related d
Robust integration of weakly anchored spatial multi-omics biorxiv.org 2026-06-14 Spatial multi-omics holds great promise for dissecting complex biological processes, though inherent technical constraints continue to limit its wides
Somatic variant detection in normal tissues from single-cell sequencing data biorxiv.org 2026-06-14 A crucial advantage of single-cell sequencing (SCS) is its ability to identify somatic variants in individual cells, enabling phylogenetic analysis of
Escherichia coli and Mammalian Cells Follow Divergent Rules for Lipid-Driven Cytosolic Accumulation biorxiv.org 2026-06-14 While lipidation is a widely observed strategy to promote membrane permeation, whether the factors governing lipid-driven accumulation are shared acro
Membrane-dependent structural organization of cowpox virus CPXV012 and its recognition of TAP biorxiv.org 2026-06-13 Cowpox virus CPXV012 inhibits MHC class I antigen presentation by interfering with TAP-dependent peptide transport, but its membrane-dependent structu
A ribosomal kinetic checkpoint governs selective mRNA recruitment biorxiv.org 2026-06-13 Translation initiation begins with recruitment of mRNA to the ribosome, yet how mRNA engagement is converted into productive initiation remains unclea
Unlocking Your Programmable and Creative RNA Sequence Designer with RDiffusion biorxiv.org 2026-06-13 As a cornerstone of the central dogma, RNA has both witnessed and actively shaped three billion years of evolution.
Continuous Capture of recombinant AAV Particles Using Twin-Column CaptureSMB biorxiv.org 2026-06-13 Recombinant adeno-associated viruses (rAAVs) have gained increasing importance in gene therapy due to their safe and precise gene delivery.
Virus-human protein-protein interactions predict viral phenotypes biorxiv.org 2026-06-13 Viral phenotypes such as host and tissue tropism are critical determinants of viral infection and transmission.
Testing the reliability of AI-generated protein structures biorxiv.org 2026-06-13 Although AlphaFold2 and its competitors have demonstrated remarkable abilities to predict protein structure, more work is needed to explore the limita
ADMETron: An AI-driven SaaS platform for comprehensive ADMET prediction and compound prioritisation biorxiv.org 2026-06-13 ONTOSIGHT(R) ADMETron is an AI-driven platform designed for rapid prediction and visualization of Absorption, Distribution, Metabolism, Excretion, and
MoE-Bind: Guiding De Novo Protein Binder Generation with Sparse Experts biorxiv.org 2026-06-13 De novo protein binder design has been dominated by structure-based pipelines that require known three-dimensional target conformations and consume su
Tightly Knotted Enzymes Inhibit Protein-Protein Aggregation biorxiv.org 2026-06-13 Neurodegenerative diseases are closely linked to aberrant protein aggregation arising from failures in cellular proteostasis, yet the physical determi
Microbial inoculation accelerates post-fire soil recovery in a mixed conifer forest biorxiv.org 2026-06-13 High-severity wildfires of increasing size and frequency result in release of carbon dioxide and loss of timber resources, reduction in biodiversity,
Molecular interplay between Integrative Mobile Elements exploiting Xer drives the evolution of cholera pandemics biorxiv.org 2026-06-13 XerC and XerD are ubiquitous bacterial recombinases dedicated to chromosome dimer resolution.
A genome wide antigen-antibody screen identifies a yeast-based therapeutic vaccine candidate for Chagas disease biorxiv.org 2026-06-13 Chagas disease is caused by Trypanosoma cruzi infection and results in decades-long, chronic, debilitating, and lethal disease.
First detection and characterization of Alongshan virus in Ixodes ricinus ticks from Italy, 2021-2022 biorxiv.org 2026-06-13 Alongshan virus (ALSV) is an emerging tick-borne segmented RNA virus belonging to the Jingmenvirus group and has been reported in humans, ticks, and v
Saturation Genome Editing reveals the functional impact of RAD51D and XRCC2 variants biorxiv.org 2026-06-13 Germline pathogenic variants in RAD51D and XRCC2, which encode RAD51 paralogs that form a heterodimer within the BCDX2 complex, confer increased cance
DNA 6mA marks transcriptionally active chromatin in malaria parasites biorxiv.org 2026-06-13 DNA N6-methyladenine (6mA) has emerged as a significant epigenetic modification across a broad range of eukaryotes, from unicellular protists to metaz
Potential for the Terminal SKP1 Glycosyltransferase to Exert Non-Enzymatic Control of SKP1 in Toxoplasma gondii biorxiv.org 2026-06-13 The SKP1/Cul1/F-Box (SCF) complex is an E3 ubiquitin ligase responsible for targeting a range of proteins for degradation by the 26S proteosome.
Intrinsically disordered N-terminal regions suppress cotranslational protein degradation biorxiv.org 2026-06-13 Intrinsically disordered regions (IDRs) of proteins are thought to be inherently sensitive to proteolysis and considered one of the key components con
ProtAff: Protein Binding Affinity Prediction via LoRA-Finetuned ESM-2 biorxiv.org 2026-06-13 Predicting the binding affinity of protein--protein interactions remains a central challenge in computational biology.
Reinforcement learning-driven unified generative framework for multi-objective RNA codon design biorxiv.org 2026-06-13 Current RNA codon design methods are limited by inefficient long-sequence processing and poor generalizability, often relying on a decoupled "generate
PertDiffBench: Benchmarking Diffusion Models for Single-Cell Perturbation Response Prediction biorxiv.org 2026-06-13 Diffusion models are increasingly used to predict transcriptional responses to perturbations, but whether they improve on simpler generative and repre
Coupled nucleoplasmic flows and envelope expansion govern the mechanical resistance of the nucleus biorxiv.org 2026-06-13 In recent year, it became clear that the cell nucleus can undergo large deformations, during immune cell migration and tumor growth.
Disruption of Myelin-Associated Glycoprotein Activity Drives Aberrant Cerebellar Neurodevelopment and Autism-Like Behaviors. biorxiv.org 2026-06-13 Immune-mediated mechanisms have emerged as important contributors to neurodevelopmental vulnerability in a subset of autism spectrum disorder (ASD) ca
Cortical geometry constrains the unimodal anchors of sensory integration biorxiv.org 2026-06-13 The human cerebral cortex is organized along a unimodal-to-transmodal hierarchy, which provides a putative substrate for the integration of sensory si
The Amygdalostriatal Transition Area Exhibits Lateral Amygdala-Like Spiking Activity and Tone-Shock Pairing-Induced Plasticity biorxiv.org 2026-06-13 During Pavlovian fear conditioning, presentation of a conditioned stimulus, such as a tone, together with an unconditioned stimulus, such as an electr
Post-translational modifications in the brain are critical contributors to Alzheimers disease neuropathology and cognitive decline biorxiv.org 2026-06-13 Post-translational modifications (PTMs) in APP and MAPT contribute to plaques and tangles in Alzheimers disease (AD).