The lysosomal cation channel TRPML1 regulates the oligodendrocyte cytoskeleton biorxiv.org 2026-06-18 Differentiating oligodendrocytes undergo dramatic morphologic alterations to transition from progenitors to mature oligodendrocytes that synthesize my
Early life Oxytocin treatment Attenuates Seizure Susceptibility in Male, but not Female, Fmr1-KO Mice biorxiv.org 2026-06-18 Fragile X syndrome (FXS) is the leading inherited cause of intellectual disability, and is frequently accompanied by seizures.
Distinct cortical patches for syntactic and semantic composition in the human brain biorxiv.org 2026-06-18 Although the brain areas for language processing are well delimited, whether lexical-semantic and syntactic processes are spatially segregated remains
Large-scale prediction of transcription factor binding across human cell types informs regulatory genomics and reveals promiscuous occupancy associated with chromatin contacts biorxiv.org 2026-06-18 Our understanding of the mechanisms regulating gene expression has been hampered by our limited knowledge of which transcription factors (TFs) bind wh
Sample preparation for mass spectrometry-based tissue (phospho)proteomics biorxiv.org 2026-06-18 This protocol describes the workflow for the preparation of tissue samples for proteome and phosphoproteome analyses using mass spectrometry.
Human striatal population state dynamics biorxiv.org 2026-06-18 Animal models reveal that striatal projection neurons (SPNs) fluctuate between discrete electrophysiological states with distinct levels of cortical i
Retinal cell mosaics in the valproate-induced rat model of autism spectrum disorder biorxiv.org 2026-06-18 Valproic acid (VPA) is a widely used antiepileptic drug that also increases the risk of neurodevelopmental disorders in the offspring of exposed mothe
Spatiotemporal Patterns and Structural Substrates of Individual Functional Variability in Youth biorxiv.org 2026-06-18 Youth is a period of emerging individuality and extensive neural remodeling, yet how functional brain individuality is organized across development re
AMaNITA: an end-to-end workflow for native tRNA nanopore sequencing data analysis biorxiv.org 2026-06-17 Transfer RNA (tRNA) molecules serve as essential adapters during protein translation.
Mechanistic Insights into Unfolding of the Mitochondrial Localization Sequence M1 of TDP-43 and Identification of a MAPKAPK2 Inhibitor as a potential binder of M1 for inhibition of TDP-43's Aberrant Mitochondrial Localization biorxiv.org 2026-06-17 Mechanistic elucidation and inhibition of the pathogenic aberrant mitochondrial localization of the RNA/DNA-binding protein, TDP-43, can help in the t
Negative and neutral interactions are prevalent in interactions between marine chitin degraders biorxiv.org 2026-06-17 Bacterial chitin degradation contributes to global carbon cycling, particularly in marine environments where it is a highly abundant polysaccharide.
Microbial community-based single-cell protein supports partial fishmeal replacement in juvenile Asian seabass diets across feeding trials and production scales biorxiv.org 2026-06-17 The growing demand for sustainable aquafeeds has intensified interest in alternative protein ingredients capable of reducing reliance on fishmeal with
High-resolution transcriptome analysis reveals a multilayered and dynamic transcriptional architecture in the archaeal virus SSV1 biorxiv.org 2026-06-17 Fuselloviridae infecting thermoacidophilic Sulfolobales are among the best-studied archaeal viruses, with the Saccharolobus Spindle Shaped Virus 1 (SS
The effects of thermal alteration on organic matter bioavailability in deeply buried marine sediments biorxiv.org 2026-06-17 A substantial fraction of marine sediments experience elevated temperatures due to burial or hydrothermal activity.
Cross-study metagenomics analysis reveals distinct microbial signatures of urinary tract infections biorxiv.org 2026-06-17 Background: Urinary tract infections (UTIs) represent a major public health concern, increasingly complicated by rising antibiotic resistance, diminis
Ferrosome Organelles Spatially Insulate a Redox-Active Ferrous Phosphate Biomineral from Cytosolic ROS Chemistry biorxiv.org 2026-06-17 Ferrosomes are recently discovered lipid-bound bacterial organelles that store iron as iron-phosphate biominerals, yet the chemical nature and physiol
Systematic evaluation of Cyanidioschyzon merolae across photobioreactor systems: Linking reactor design to biomass production and biochemical composition biorxiv.org 2026-06-17 Extremophilic red microalgae are promising platforms for sustainable biotechnology, combining robust growth under selective thermoacidophilic conditio
From parental antibiotics to offspring locomotion: L-norvaline links microbiome disruption to intergenerational reprogramming in Drosophila biorxiv.org 2026-06-17 The maternal microbiome during oogenesis is a critical but underappreciated regulator of offspring physiology.
Spatial organization of self-assembled G-quadruplex nucleic acids tunes extracellular electron transfer in pathogenic biofilms biorxiv.org 2026-06-17 Extracellular electron transfer (EET) allows bacteria to sustain metabolism when soluble electron acceptors such as oxygen are scarce, a situation typ
Insertions in universal proteins confirm that Nanohaloarchaea belong to DPANN-Archaea and suggest several new major archaeal clades. biorxiv.org 2026-06-17 The phylogenetic position of Nanohaloarchaea has been debated, these nanosized archaea being alternatively proposed as sister group to Haloarchaea, me
DesignMaster: A Multi-Conditional Diffusion Framework for Rational PROTAC Design biorxiv.org 2026-06-17 Motivation: Proteolysis-targeting chimeras (PROTACs) enable targeted protein degradation through ternary complex formation with E3 ubiquitin ligase.
DNA-binding specificity recognition from predicted homologous protein-DNA structures biorxiv.org 2026-06-17 Predicting protein DNA-binding specificity is essential for understanding gene regulation and disease mechanisms.
Borrelia burgdorferi bb0164 encodes a Na+/Ca2+ antiporter homolog with a novel role in Mn2+ homeostasis and infectivity biorxiv.org 2026-06-17 Borrelia burgdorferi, the Lyme disease causative agent, relies on trace metals for motility, growth, and virulence in the absence of metal transport h
Toward understanding drivers of specialized metabolism in Actinomycetota: insights from 1432 transcriptomics datasets for 132 strains biorxiv.org 2026-06-17 Actinomycetota are major sources of specialized metabolites with applications in drug discovery and agriculture, yet much of their biosynthetic potent
A strain-specific metabolic role for the UDP-glucose 4-epimerase Uge3 in Aspergillus fumigatus virulence biorxiv.org 2026-06-17 Expression of a fungal-specific sub-telomeric gene, hrmA, in Aspergillus fumigatus is important for a colony biofilm morphology termed H-MORPH, increa
High-Throughput Profiling of Bacterial Respiration Using the Resipher Reveals Functional Responses to Nutrients and Antibiotics biorxiv.org 2026-06-17 Oxygen consumption is a direct functional readout of bacterial respiration and metabolic state, yet existing methods for quantifying oxygen dynamics a
Ecological inference and contaminant detection from fungal microbiome data with q2-fungal-traits biorxiv.org 2026-06-17 Fungi are key members of microbial communities, yet microbiome surveys often lack trait-based information required for ecologically meaningful interpr
RNA-dependent aggregation of a common TEV protease variant alters in vitro biomolecular condensate reconstitution biorxiv.org 2026-06-17 Biomolecular condensates formed by liquid-liquid phase separation (LLPS) are commonly studied in vitro using protease-mediated removal of solubilizing
mRNA concentration-dependent translation enables rapid and sharp patterning in resource constraint Drosophila embryos biorxiv.org 2026-06-17 One of the fundamental challenges in protein expression during embryogenesis is the establishment of dynamic protein patterns under the resource const
An Antibiotic-Treated Mouse Model Reveals the Progression of Intestinal Botulism biorxiv.org 2026-06-17 Intestinal botulism, including infant botulism and adult intestinal toxemia botulism, is a life-threatening disease caused by intestinal infection wit
Signaling input from the polar flagellum uncouples quorum sensing from cell-density regulation in Vibrio species biorxiv.org 2026-06-17 Quorum sensing (QS) is traditionally recognized as a signaling mechanism that monitors cell density to coordinate bacterial group behaviors.
Virus-host interactions on volcanic ash from Mount Etna biorxiv.org 2026-06-17 Volcanic ash represents an extreme and dynamic habitat, yet it hosts diverse microbial communities with largely unexplored viral diversity.
Antibiofilm peptide incorporated PHBV-PLLA nanofibrous mat for wound infection treatment and healing biorxiv.org 2026-06-17 Tissue regeneration using bioactive biomaterials has made great progress in the field of wound healing.
Servo-Actuated 3D-Printed Disposable Microvalves for Automated, Scalable Organoid Culture in Standard Incubators biorxiv.org 2026-06-17 Automation of organoid and cell culture processes is essential for achieving scalable and standardized experimentation in regenerative medicine and st
Cyclic stretch inhibits cell invasion in 3D scaffolds biorxiv.org 2026-06-17 Background: The development of clinically viable tissue-engineered heart valves (TEHVs) remains limited by inconsistent host cell infiltration.
A Pro-Regenerative Petroleum Jelly-Based, Copper-Doped Bioactive Glass Ointment for Impaired Wound Healing in Metabolic Syndrome biorxiv.org 2026-06-17 Chronic wound healing is often impaired in conditions such as metabolic syndrome, requiring effective therapeutic interventions to promote tissue rege
Posterior-calibrated multimodal motor states reveal longitudinal and imaging-associated heterogeneity in Parkinson's disease biorxiv.org 2026-06-17 Parkinson's disease (PD) motor heterogeneity is commonly summarized by hard subtype labels, although clinical states vary longitudinally, severity can
Correcting spatial transcriptomics data affected by a prevalent transcript leakage problem across platforms, species, and tissues biorxiv.org 2026-06-17 Spatial transcriptomics has been widely applied to study the spatial distribution of cell types, cell states, and specific gene expression in tissue s
Stability and Compressibility of Tear Film Lipid Layer: Impact of Benzalkonium Chloride Presence biorxiv.org 2026-06-17 PURPOSE.: Benzalkonium chloride (BAK), a common preservative in eye drops, has a major side effect of dry eye.
Stratigraphic Paleobiology of Carbonate Systems biorxiv.org 2026-06-17 Stratigraphic paleobiology is a newly established interdisciplinary approach, which has demonstrated that the fossil record is a joint expression of b
Protein expression level of P2RY12 correlates with survival in Non-Small Cell Lung Cancer and exhibits diagnostic potential for Squamous Cell Carcinomas of the Lung biorxiv.org 2026-06-17 Context: P2Y12 receptor (P2RY12), mainly expressed on platelets, is known for its central role in hemostasis.
Reactivating a schizophrenia risk gene-enriched prefrontal ensemble suppresses decision noise biorxiv.org 2026-06-17 Schizophrenia genetics and single-cell transcriptomics implicate prefrontal excitatory neurons and synaptic programs, but how these vulnerabilities al
Ventricular Expansion Couples Hyperosmotic Stress to Thirst biorxiv.org 2026-06-17 Thirst is thought to arise from osmotic signals detected by circumventricular organs, yet how hyperosmotic stress activates mechanosensitive channels
Colorful connections: pigment-based plumage and breeding condition are associated with gut microbiome variation in the Common Yellowthroat biorxiv.org 2026-06-17 Carotenoid- and melanin-based plumage coloration traits are key signals in avian communication and sexual selection as they are often thought to provi
Global variations of Light Use Efficiency in Forests Jointly Driven by Plant Traits and Climatic Conditions biorxiv.org 2026-06-17 Forests are essential to the global carbon cycle with light use efficiency (LUE) as a key parameter for assessing carbon sequestration capacity.
Chemoselective Halogenation of Premarineosin A for Next-Generation Antimalarial Development biorxiv.org 2026-06-17 Premarineosin A undergoes rapid, chemoselective C12 halogenation under mild conditions, providing brominated, chlorinated, fluorinated, and iodinated
A DIA-based quantitative crosslinking mass spectrometryframework for dynamic structural proteomics biorxiv.org 2026-06-17 Proteins undergo dynamic conformational rearrangements and interactions that are central to their biological functions.
Q-DOAS: A Proximity Quenching Assay for Real-Time Detection of Early Protein Aggregation Events biorxiv.org 2026-06-17 Accurate measurement of protein aggregation is essential for studying neurodegenerative diseases.
Ubiquitin Chloromethylketone Probe Enables Activity-based, Selective Protein Profiling of E2 Ubiquitin Conjugating Enzymes biorxiv.org 2026-06-17 Ubiquitin (Ub) conjugating enzymes (E2s) are central to Ub signaling, yet their systematic activity-based profiling remains challenging due to the wea
Universal oligo adapters for high-efficiency DNA-barcoded antibody panel generation biorxiv.org 2026-06-17 DNA-barcoded antibodies are central to a broad range of spatial and dissociative assays and applications, including multiplexed imaging, single-cell p