Magnetic Tweezers Experiments Reveal Increased Mechanical Sensitivity of the F2561Y Mutant in Von Willebrand Factor biorxiv.org 2026-06-06 The large glycoprotein von Willebrand factor (VWF) is essential for primary hemostasis.
Simulation of transcription factor clustering in nuclei from molecular kinetics biorxiv.org 2026-06-06 Transcription factors form clusters often described as condensates that exhibit emergent biophysical properties.
Temporal coding expands the bandwidth of GPCR-mediated neuromodulation biorxiv.org 2026-06-06 The advent of modern transcriptomic approaches has revealed a breadth of neuromodulatory G protein-coupled receptor (GPCR) co-expression far exceeding
iSBEM: An Open-Source Workflow for Automated ROI Targeting in Volume Electron Microscopy biorxiv.org 2026-06-06 Serial Block Face - Scanning Electron Microscopy (SBF-SEM) is a volume EM method suited to investigate the 3D architecture of tissues and even entire
Metformin Redirects Autophagy from Bulk Turnover to Mitochondrial Clearance biorxiv.org 2026-06-06 Metformin is the most widely prescribed antidiabetic drug and an active candidate for repurposing in oncology.
Somatic cells non-autonomously control germline incomplete cytokinesis through FGF signaling biorxiv.org 2026-06-06 Across species, germ cells divide and differentiate as interconnected units, termed cysts.
Organ-specific fibroblast dynamics revealed via an integrated experimental-computational framework biorxiv.org 2026-06-06 Fibrosis is a progressive pathological process driven by dysregulated fibroblast activity and excessive extracellular matrix deposition, leading to sc
Constitutive plasma membrane interaction of active Rho GEF Ect2 inhibits cortex contraction pulses biorxiv.org 2026-06-06 During cell division, multiple guanine nucleotide exchange factors (GEFs), including the mitotic regulator Ect2 and Lbc-type GEFs, regulate the dynami
Vacuole pH loss triggers ESCRT-dependent plasma membrane remodeling to prevent amino acid toxicity biorxiv.org 2026-06-06 Loss of lysosomal or vacuolar acidity is a hallmark of aging, metabolic dysfunction, and cellular stress, yet how cells adapt to this condition remain
Piezo1 transduces mechanical signals to inhibit osteoclast fusion and coordinate bone homeostasis biorxiv.org 2026-06-06 Osteoclasts originate from bone marrow-derived macrophages, and their maturation relies on fusion of pre-osteoclasts into mature osteoclasts that main
Nir1-Nir2 Heterodimerization Confers Robustness to the Phosphoinositide Cycle biorxiv.org 2026-06-06 The phosphatidylinositol (PI) cycle maintains plasma membrane (PM) phosphatidylinositol 4,5-bisphosphate (PIP2) levels to sustain signaling and cellul
Matrix nucleotide homeostasis couples energetic state to mitochondrial translation biorxiv.org 2026-06-06 Mitochondrial protein synthesis must adapt to fluctuations in organellar energetic state to sustain oxidative phosphorylation and cellular homeostasis
Molecular basis of competence for neural induction in the chick embryo biorxiv.org 2026-06-06 Competence is the capacity of a cell or tissue to respond to a specific inducing signal from a neighbouring tissue, by changing its fate in a specific
Rab8a-positive vesicles transport Wnt8a along cytonemes in zebrafish embryogenesis. biorxiv.org 2026-06-06 Wnt signalling is a conserved pathway that orchestrates key developmental processes by regulating cell fate, proliferation, and tissue organisation.
MarkerScout: A Disease-Agnostic Machine Learning Framework for Biomarker Prediction from Multi-Scale Mechanistic Models biorxiv.org 2026-06-05 Identifying robust biomarkers from high-dimensional biomedical data is a central challenge in translational research, but candidate rankings produced
The canine respiratory epithelium is a permissive ecosystem for influenza interspecies transmission and emergence biorxiv.org 2026-06-05 The outcome of virus spillover ranges from dead-end infections to pandemics and is underpinned by host-pathogen interactions as well as evolutionary a
Galectin-3 recruitment at the Mycobacterium tuberculosis-containing phagosome is critical in macrophage but dispensable in epithelial cells biorxiv.org 2026-06-05 Mycobacterium tuberculosis (Mtb) virulence relies in part on its ability to induce phagosomal membrane rupture, enabling bacterial access to the host
Longitudinal RNA Seq analyses reveal the prominent role of Vagococcus in broiler meat spoilage microbiome biorxiv.org 2026-06-05 Background Raw broiler meat products are highly perishable, and microbial activity is the main factor limiting their shelf lives.
Inorganic sulfate transport by the Mycobacterium tuberculosis PE22/PPE36 complex biorxiv.org 2026-06-05 Mycobacterium tuberculosis (Mtb) encodes two multigene families with 169 members that are exclusive to mycobacteria, the pe and ppe genes.
Disrupting IFIT1-STAT2 viral evasion synergy yields live-attenuated flavivirus with enhanced immune priming biorxiv.org 2026-06-05 Diverse human viruses deploy a common immune-evasion strategy: viral 2'-O-methylation coupled with STAT2 antagonism.
Variable zinc concentrations among commercially available Mueller-Hinton Broth brands affects SIR interpretations during broth microdilution in vitro antimicrobial susceptibility testing with the novel metallo-beta-lactamase inhibitor APC148 biorxiv.org 2026-06-05 The well-known discrepancy between the in vitro and in vivo efficacy of {beta}-lactam antibiotics in metallo-{beta}-lactamase (MBL) containing Gram-ne
Minimal effective dose of telacebec administered orally in a murine model of leprosy biorxiv.org 2026-06-05 Leprosy treatment requires prolonged therapy with challenging patient follow-up. New regimens are needed to simplify current treatments.
MicroGrowAgents: An Agentic AI System for Microbial Cultivation Engineering biorxiv.org 2026-06-05 Microbial cultivation optimization remains labor-intensive and inefficient, requiring extensive experimental screening to identify suitable growth con
Characterization of velvet DNA-binding region by Aspergillus nidulans VelB biorxiv.org 2026-06-05 Velvet regulators characterized by a conserved velvet domain function as a central hub that coordinately govern fungal development, secondary metaboli
Quantitative stable isotope probing to measure in situ growth of protist populations biorxiv.org 2026-06-05 Soil protists shape microbial food webs and nutrient cycling, yet methods for measuring their population growth in soil have lagged behind the taxonom
Selective inhibition of respiratory complex I reveals a bioenergetic vulnerability in Francisella biorxiv.org 2026-06-05 F. tularensis is a highly infectious Gram-negative bacterial pathogen that causes tularemia, a re-emerging zoonosis of public health concern.
Flagellar system translocates T3SS effectors critical for Salmonella infection biorxiv.org 2026-06-05 Type 3 secretion system (T3SS) injectisomes, the essential virulence factors of many bacterial pathogens, are thought to have evolved from the flagell
Microbial community profiles of the snake cloaca in the presence and absence of Chlamydiota biorxiv.org 2026-06-05 Chlamydiota are obligate intracellular bacteria detected in snake cloacal microbiota, yet their biological significance remains poorly understood.
Transcription factor CCTF1 plays a decisive role in regulating the proteasome activity during archaeal cell division biorxiv.org 2026-06-05 Archaea of the order Sulfolobales exhibit a highly ordered cell cycle progression similar to that of eukaryotes.
Structural and Catalytic Architecture of the Cysteine Synthase Cys1a biorxiv.org 2026-06-05 Antifungal drug resistance in Aspergillus fumigatus is a major global health concern.
Intraspecific genetic variation modulates immune responses to acute heat exposure in an aquatic ectotherm biorxiv.org 2026-06-05 Climate change-induced heatwaves threaten ectotherms, whose physiology is tightly coupled to ambient temperature.
Night-time control of carbohydrate availability in grasses differs radically from that in Arabidopsis biorxiv.org 2026-06-05 Grass leaves reportedly accumulate sucrose during the day then export it to other organs at night. The control of export at night is not understood.
Modeling the journey as well as the destination: a control theory account of rotational navigation biorxiv.org 2026-06-05 Navigation requires estimating heading and transforming these estimates into actions.
Rapid Determination of Drug-to-Antibody Ratios in Antibody Drug Conjugates Using Ultrafast Microdroplet Digestion Technology biorxiv.org 2026-06-05 Accurate determination of drug to antibody ratios (DARs) is essential for the development, quality control, and performance evaluation of antibody dru
Split intein vectors permit oversize FLIM-FRET biosensor neuronal expression biorxiv.org 2026-06-05 Adeno-associated virus (AAV) packaging limits constrain the design, performance, and in vivo application of genetically encoded biosensors.
Maturation of Gait: Identification of Locomotor Profiles from Early Childhood to Adulthood biorxiv.org 2026-06-05 Background: Human gait is a key marker of motor development.
Label-Free AI-Classification of Subcellular Organelles Based on Optical Photothermal Infrared Images biorxiv.org 2026-06-05 Cells maintain homeostasis by dynamically reorganizing their organelles to tune metabolism in response to stress.
Extrachromosomal DNA as a Causal Instrument for Spatial Multi-Omics biorxiv.org 2026-06-05 Spatial transcriptomics, multiplex imaging, and computational pathology now map tissue organization at cellular resolution, but the analyses applied t
Lifespan Trajectories of Resting State EEG power biorxiv.org 2026-06-05 Trajectories of resting state monopolar EEG power were analyzed using the means, variances, and correlations of data in seven frequency bands obtained
Aβ Aggregates Bind the U1 Spliceosomal Ribonucleoprotein in Alzheimer Disease Brain biorxiv.org 2026-06-05 Missplicing due to U1 small nuclear ribonucleoprotein (U1 snRNP) insolubilization and dysfunction has been identified in Alzheimer disease (AD) brain.
A unified developmental framework of the human placenta in its uterine environment in vivo and in vitro biorxiv.org 2026-06-05 Despite advances in single-cell profiling of the human placenta, the genetic programs governing its physiological remodeling throughout gestation rema
Genomic Dimensionality Bounds Mixed-Model Association Power and Fine-Mapping Resolution biorxiv.org 2026-06-05 Mixed-model genome-wide association studies (GWAS) behave differently in livestock than in humans, yet a unified explanation is lacking.
Loss of the Y chromosome drives epigenetic and transcriptomic plasticity in lung adenocarcinoma biorxiv.org 2026-06-05 Loss of the Y chromosome (LOY) is associated with poor survival across multiple solid tumors, yet the underlying molecular mechanisms remain poorly un
Afrothismia lovettii sp. nov. (Afrothismiaceae) Critically Endangered (Possibly Extinct) and endemic to the Kihansi Falls Forest, Tanzania biorxiv.org 2026-06-05 The fully mycotrophic (or mycoheterotrophic) non-photosynthetic Afrothismia lovettii Cheek sp. nov.
Orientation Dependence of R2' in White Matter: Digital Characterization, Modelling and Implications for Studying Brain Physiology biorxiv.org 2026-06-05 Introduction R2* is the transverse relaxation rate of tissue, influenced by local magnetic field inhomogeneities arising from susceptibility differenc
HDAC3 inhibition stabilizes the IL-37 receptor module to enhance anti-inflammatory signaling in cystic fibrosis airway epithelium biorxiv.org 2026-06-05 Airway inflammation in cystic fibrosis (CF) persists despite advances in CFTR modulator therapy.
Phylogeny, thermal seasonality, and life history shape trait variation in annual and perennial barley relatives biorxiv.org 2026-06-05 Summary {middle dot} There is growing interest in developing perennial cereal crops for sustainable production, yet key differences in trait syndromes
Identification of novel enolase negative Segatella copri subspecies supports notion of Segatella copri speciation via alternative phosphoenolpyruvate synthesis pathways biorxiv.org 2026-06-05 Segatella copri is characterised as a prominent glycolytic plant-based fiber utiliser within the human gut.
Rational engineering of sdAb-based CAR T cells targeting BCMA enhances antitumor efficacy and persistence in Multiple Myeloma biorxiv.org 2026-06-05 BCMA-directed CAR T therapies have transformed the treatment of relapsed/refractory multiple myeloma (MM), yet durable responses remain limited by ins
Cellpin enables reference-based imputation and denoising of spatial transcriptomes biorxiv.org 2026-06-05 Spatially resolved transcriptomics enables gene expression profiling within tissue architecture, but targeted panels leave much of the transcriptome u